The role of miR-135-modified adipose-derived mesenchymal stem cells in bone regeneration

Xie, Qing; Zi, Wenjie; Zhou, Huifang; Zhang, Yu; Huang, Yazhuo; Sun, Hao; Bi, Xiaoping; Wang, Yefei; Shi, Wodong; Gu, Ping; Fan, Xianqun

doi:10.1016/j.biomaterials.2015.10.042

Cited by 92 publications

(62 citation statements)

References 60 publications

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“…Among all miRNAs, miR-135 has been shown to be involved in regulation of cell differentiation, regeneration, and carcinogenesis [29][30][31][32][33][34][35][36]. However, a role of miR-135 in the protective effects by H 2 S against Parkinson's disease has not been shown previously.…”

Section: Introductionmentioning

confidence: 99%

Involvement of microRNA-135a-5p in the Protective Effects of Hydrogen Sulfide Against Parkinson's Disease

Liu

Liao

Quan

et al. 2016

Cell Physiol Biochem

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Background/Aims: Development of effective therapeutic drugs for Parkinson's disease is in great need. During the progression of Parkinson's disease, Rho-associated protein kinase 2 (ROCK2) is activated to promote neurodegeneration. Hydrogen sulfide (H₂S) has a neuroprotective effect during the neural injury of Parkinson's disease. However, the mechanisms that underlie the effects of ROCK2 and H₂S remain ill-defined. In the current study, we addressed these questions. Methods: We used a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse subacute model of Parkinson's disease to study the effects of H₂S on astrocytic activation in the mouse striatum, on the levels of tyrosine-hydroxylase (TH)-positive neuron loss, on the apomorphine-induced rotational behavior of the mice, and on the changes in ROCK2 and miR-135a-5p expression. Plasmid transfection was applied to modify miR-135a-5p levels in a neuronal cell line HCN-1A. Bioinformatics analysis was performed to predict the relationship between ROCK2 and miR-135a-5p in neuronal cells, and then was confirmed by luciferase reporter assay. Results: H₂S alleviated MPTP-induced astrocytic activation in the mouse striatum, alleviated the increases in TH-positive neuron loss, and improved the apomorphine-induced rotational behavior of the mice. H₂S significantly attenuated the increases in ROCK2 and the decreases in miR-135a-5p by MPTP. MiR-135a-5p targeted the 3'-UTR of ROCK2 mRNA to inhibit its translation in neuronal cells. Conclusion: MiR-135a-5p-regulated ROCK2 may play a role in the protective effects of hydrogen sulfide against Parkinson's disease.

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Section: Introductionmentioning

confidence: 99%

Involvement of microRNA-135a-5p in the Protective Effects of Hydrogen Sulfide Against Parkinson's Disease

Liu

Liao

Quan

et al. 2016

Cell Physiol Biochem

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“…In comparison with retroviruses, lesser insertional mutagenesis risks are reported with the use of lentiviruses, [44] thus perhaps encouraging recent developments with lentiviral engineering of cells prior to seeding onto biomaterial scaffolds. [45][46][47] ADVs and AAVs delivering miRNAs are less explored in biomaterial-based RM approaches, although their use in traditional in vitro transductions with miRNA-expressing cassettes has been discussed in other reviews. [48] Figure 3.…”

Section: Viral Vectorsmentioning

confidence: 99%

“…[107] Many distinct scaffold types have been tuned to serve the purpose of miRNA delivery, including several hydrogels, [58,92,106,[108][109][110][111][112][113][114] electrospun fibers, [63,[115][116][117][118] and more prolifically porous or spongy scaffolds. [46,47,50,54,55,96,112,[119][120][121][122][123][124][125][126][127][128][129][130] Before reviewing the details of their applications, summarized in Table 2 and described further in the next section, we will discuss the key characteristics making these materials amenable to miRNA delivery. First very general qualities must be fulfilled: biocompatibility, bioresorbability and ease of fabrication.…”

Section: Scaffolds For Microrna Deliverymentioning

confidence: 99%

“…Lentivirus has been used to deliver miR-135 to ASCs for assessment of osteogenesis and bone repair in vitro and in vivo. [47] For in vivo assessment, transduced ASCs were combined with poly(sebacoyl diglyceride) (PSeD) scaffolds and implanted in critical-sized rat calvarial defects. Results correlated with in vitro assessment and showed that miR-135 mimic treatment resulted in significant new bone formation with elevated BMD, increased trabeculae number (Tb.N) and enhanced mineralization staining.…”

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

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Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Curtin

Castaño

O’Brien

2017

Adv Healthcare Materials

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The field of "regenerative medicine" (RM) was conceptually developed to aid the body's natural capacity to self-repair, a capacity which is impaired with age and in cases of disease or injury. [1] RM is a vast and multifaceted area of medicine, often microRNA-based therapies are an advantageous strategy with applications in both regenerative medicine (RM) and cancer treatments. microRNAs (miRNAs) are an evolutionary conserved class of small RNA molecules that modulate up to one third of the human nonprotein coding genome. Thus, synthetic miRNA activators and inhibitors hold immense potential to finely balance gene expression and reestablish tissue health. Ongoing industrysponsored clinical trials inspire a new miRNA therapeutics era, but progress largely relies on the development of safe and efficient delivery systems. The emerging application of biomaterial scaffolds for this purpose offers spatiotemporal control and circumvents biological and mechanical barriers that impede successful miRNA delivery. The nascent research in scaffold-mediated miRNA therapies translates know-how learnt from studies in antitumoral and genetic disorders as well as work on plasmid (p)DNA/siRNA delivery to expand the miRNA therapies arena. In this progress report, the state of the art methods of regulating miRNAs are reviewed. Relevant miRNA delivery vectors and scaffold systems applied to-date for RM and cancer treatment applications are discussed, as well as the challenges involved in their design. Overall, this progress report demonstrates the opportunity that exists for the application of miRNA-activated scaffolds in the future of RM and cancer treatments.Regenerative Medicine

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“…It is believed that, after pretreatment, MSCs may be the ideal drug or gene delivery media [166, 167]. In addition, MEX in different growth stages or culture media contain different RNAs and proteins, so the processes of extraction and collection also need to be standardized [168, 169]. …”

Section: The Role Of Mesenchymal Stem Cell-derived Exosomes and MImentioning

confidence: 99%

Mesenchymal Stem Cells as a Prospective Therapy for the Diabetic Foot

Chen

Liang

2016

Stem Cells International

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The diabetic foot is a serious complication of diabetes. Mesenchymal stem cells are an abundant source of stem cells which occupy a special position in cell therapies, and recent studies have suggested that mesenchymal stem cells can play essential roles in treatments for the diabetic foot. Here, we discuss the advances that have been made in mesenchymal stem cell treatments for this condition. The roles and functional mechanisms of mesenchymal stem cells in the diabetic foot are also summarized, and insights into current and future studies are presented.

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The role of miR-135-modified adipose-derived mesenchymal stem cells in bone regeneration

Cited by 92 publications

References 60 publications

Involvement of microRNA-135a-5p in the Protective Effects of Hydrogen Sulfide Against Parkinson's Disease

Involvement of microRNA-135a-5p in the Protective Effects of Hydrogen Sulfide Against Parkinson's Disease

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Mesenchymal Stem Cells as a Prospective Therapy for the Diabetic Foot

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