The role of microRNAs in melanoma

Luo, Chonglin; Weber, Claudia E.M.; Osen, Wolfram; Bosserhoff, Anja‐Katrin; Eichmüller, Stefan B.

doi:10.1016/j.ejcb.2014.02.001

Cited by 39 publications

(28 citation statements)

References 143 publications

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“…In fact, Esquela-Kerscher and colleagues (13) could show that miRNAs can act either as oncogenes or tumor suppressors depending on the function of their target molecules and on the regulation of downstream signaling pathways. Thus, a differentiation between tumor-promoting oncomirs and protective tumor suppressors, as pointed out in various reviews (14,15) has meanwhile prevailed.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, miRNAs represent complex regulators of target protein expression that can affect cellular downstream signaling pathways (15). To identify putative miRNA-binding sites within the 3 0 UTR of cellular target mRNAs, miR-339-3p was subjected to in silico analyses using miRBase, applying the miRanda software in combination with TargetScan (27,28).…”

Section: Validation Of Mir-339-3p As Inhibitor Of Melanoma Cell Invasionmentioning

confidence: 99%

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miR-339-3p Is a Tumor Suppressor in Melanoma

et al. 2016

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Determinants of invasion and metastasis in cancer remain of great interest to define. Here, we report the definition of miR-339-3p as a novel tumor suppressive microRNA that blocks melanoma cell invasion without affecting cell survival. miR-339-3p was identified by a comprehensive functional screen of a human miRNA mimetic library in a cell-based assay for invasion by the melanoma cell line A375. miR-339-3p was determined as a strong inhibitor of invasion differentially expressed in melanoma cells and healthy melanocytes. MCL1 was defined as a target for downregulation by miR-339-3p, functioning through direct interaction with the 3 0 untranslated region of MCL1 mRNA. Blocking miR-339-3p by an antagomiR was sufficient to increase melanoma cell invasion, an effect that could be phenocopied by RNAi-mediated silencing of MCL1. In vivo studies established that miR-339-3p overexpression was sufficient to decrease lung colonization by A375 melanoma cells in NSG mice, relative to control cells. Overall, our results defined miR-339-3p as a melanoma tumor suppressor, the levels of which contributes to invasive aggressiveness.

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Section: Introductionmentioning

confidence: 99%

Section: Validation Of Mir-339-3p As Inhibitor Of Melanoma Cell Invasionmentioning

confidence: 99%

miR-339-3p Is a Tumor Suppressor in Melanoma

et al. 2016

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“…16 Upregulated miRNA levels, for example miR-182 and miR-149, and downregulated levels of miRNAs, for example miR-26a and miR-101, have been shown to be involved in the development and progression of malignant melanoma. 5,17 Overexpression of miR-21 is associated with the progression, metastasis and poor prognosis of many types of tumours via its regulation of the phosphatase and tensin homologue (PTEN), the tissue inhibitor of metalloproteinase 3 and PDCD4 genes. [6][7][8] A study of 127 primary melanoma tissues demonstrated that levels of miR-21 were significantly increased compared with benign naevi.…”

Section: Discussionmentioning

confidence: 99%

“…4 Studies reveal that several miRs play important roles in the tumourigenesis, progression and metastasis of malignant melanoma, such as miR-137, miR-101, let-7, miR-26a, miR-221/222, miR-155, miR-21, miR-182, miR-30b/30d, and miR-214. 5 The overexpression of miR-21 has already been shown to be associated with progression, metastasis and poor prognosis in a variety of cancers, such as colon cancer, pancreatic cancer, and breast cancer. [6][7][8] Programmed cell death 4 (PDCD4), a tumour suppressor gene, has been shown to be downregulated or absent in different human cancers and this downregulation has been associated with a poor cancer outcome.…”

Section: Introductionmentioning

confidence: 99%

Expression and clinicopathological significance of microRNA-21 and programmed cell death 4 in malignant melanoma

2015

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Objective: To measure levels of microRNA (miR)-21 and its target gene, programmed cell death 4 (PDCD4), in samples of human cutaneous malignant melanoma and normal non-malignant control skin. Methods: Relative levels of miR-21 and PDCD4 mRNA were measured using a quantitative realtime reverse transcription-polymerase chain reaction. Correlations between the levels of the two molecules and the clinicopathological characteristics of malignant melanoma were analysed. Results: A total of 67 cases of human cutaneous malignant melanoma were analysed and compared with 67 samples of normal nonmalignant control skin. Compared with normal skin samples, the relative level of miR-21 was significantly higher and the relative level of PDCD4 mRNA was significantly lower in the melanoma specimens. A significant negative correlation between PDCD4 mRNA and miR-21 was demonstrated in malignant melanoma (r ¼ À0.602). Elevated miR-21 and reduced PDCD4 mRNA levels were both significantly correlated with increased tumour size, a higher Clark classification level and the presence of lymph node metastases in malignant melanoma. Conclusions: These findings suggest that miR-21 and PDCD4 might be potential biomarkers for malignant melanoma and might provide treatment targets in the future.

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“…It is well-known that melanoma is an aggressive cancer with increased proliferative ability (18). Previous studies have revealed that miR-185 functions as a tumor suppressor in numerous types of cancer (9,19).…”

Section: Mir-185 Functions As a Tumor Suppressor In Melanoma B16mentioning

confidence: 99%

miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma

Tian

Yang

et al. 2017

Oncology Letters

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Abstract.Melanoma is an aggressive malignancy that is increasingly common and exhibits a poor patient survival rate. Radiotherapy is the primary option for patients with melanoma, particularly those who are not candidates for surgery; however, the therapeutic effect is limited due to the relative radioresistance of melanoma to ionizing radiation (IR). It has been reported that microRNAs (miRNAs) serve a vital role in determining the radiosensitivity of tumors; however, little is known concerning the radiosensitization of melanoma using miRNA. In the present study, the radiosensitization effect of miRNA 185 (miR-185), which has been demonstrated to reduce renal cancer radioresistance, was investigated in B16 cells, a skin melanoma cell line derived from C57/BL mice, was investigated. Cell proliferation and scratch wound healing assays were used to determine the proliferative and migratory abilities of B16 cells. Annexin V/propidium iodide double staining was used to determine the apoptosis induced by IR. A tumor formation assay was performed to determine the radiosensitization effect of miR-185 on melanoma cells in vivo. Proliferation marker protein Ki-67, and hematoxylin and eosin staining were used to assess the proliferative activity and histological changes, respectively. The results of the present study demonstrated that miR-185 suppresses cellular proliferation and migration, and enhances IR-induced apoptosis, and the inhibition of proliferation and migration, in vitro and in vivo, which provides an insight into understanding the radiosensitization of melanoma using miRNA.

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The role of microRNAs in melanoma

Cited by 39 publications

References 143 publications

miR-339-3p Is a Tumor Suppressor in Melanoma

miR-339-3p Is a Tumor Suppressor in Melanoma

Expression and clinicopathological significance of microRNA-21 and programmed cell death 4 in malignant melanoma

miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma

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