The Role of microRNAs in Pulp Inflammation

Muñoz‐Carrillo, José Luis; Vázquez-Alcaraz, Silverio Jafet; Vargas-Barbosa, Jazmín Monserrat; Ramos-Gracia, Luis Guillermo; Alvarez-Barreto, Israel; Medina-Quiroz, Alejandro; Díaz-Huerta, Karla Karina

doi:10.3390/cells10082142

Cited by 17 publications

(9 citation statements)

References 175 publications

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“…MicroRNAs are differentially expressed in dental pulp tissues, and they are involved in cell differentiation, proliferation and apoptosis (Munoz‐Carrillo et al, 2021). According to Maqbool et al, miRNAs such as miR‐125a‐3p, miR‐21, miR‐410 and miR‐30b were downregulated among DPC, while miRNAs such as miR‐223‐3p, miR‐146a, miR‐506, miR‐21‐5p and let‐7c‐5p were significantly upregulated among the DPC (Maqbool et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of MicroRNA‐155 aggravates pulpitis by targeting kinesin superfamily Proteins‐5C based on illumina high‐throughput sequencing

Jiang

Chen

et al. 2023

Int Endodontic J

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Aim To investigate the regulatory role of miR‐155 and Kinesin Superfamily Proteins‐5C (KIF‐5C) in the progression of pulpitis based on bioinformatic analysis. Methodology Normal pulp tissues and pulpitis pulp tissues were collected and subjected to high‐throughput sequencing and the differentially expressed miRNAs were determined. An in vitro and in vivo pulpitis model was established. HE, IHC staining and histological evaluation were used to verify the inflammatory state of human and mouse pulp tissues. The mRNA expression of IL‐1β and TGF‐β1 were determined by RT‐qPCR and protein expression of IL‐1α, IL‐4, IL‐8, IL‐13, IFN‐γ, IL‐6, IL‐10 and MCP‐1 were determined by protein chip. The target genes of miR‐155 were predicted by miRanda database and verified by Dual‐luciferase reporter assay, RT‐qPCR and western blotting. MiR‐155 lentivirus were used to upregulate or downregulate miR‐155 and the siRNA of KIF‐5C was used to downregulate KIF‐5C. The expression of miR‐155 or KIF‐5C was determined by RT‐qPCR. All statistics were analysed using GraphPad prism 8.2. Results The high‐throughput sequencing results showed that 6 miRNAs (miR‐155, miR‐21, miR‐142, miR‐223, miR‐486, miR‐675) were significantly upregulated in diseased human pulp tissues, and miR‐155 was significantly elevated among the six miRNAs. RT‐qPCR results demonstrated that miR‐155 expression was upregulated in human pulpitic tissue, mice pulpitic tissue and LPS‐HDPCs. IL‐1β was increased while TGF‐β1 was decreased in lenti‐miR‐155 transfected LPS‐HDPCs. Analysis of protein chip results indicated that lenti‐miR‐155 transfected LPS‐HDPCs produced higher levels of IL‐8, IL‐6, MCP‐1. The opposite results were obtained when miR‐155 was inhibited. Through miRanda database screen and Dual‐luciferase reporter assay, the target gene (KIF‐5C) of miR‐155 was identified. In lenti‐miR‐155 transfected LPS‐HDPCs, the expression of KIF‐5C was downregulated. However, when shRNA‐miR‐155 was transfected to LPS‐HDPCs, the opposite result was obtained. Silent RNA was used to knock down KIF‐5C, the results showed that when both KIF‐5C and miR‐155 were knocked down simultaneously, the downregulated expression of inflammatory factors observed in LPS‐HDPCs following miR‐155 knockdown was rescued. Conclusion MiR‐155 plays an important role in promoting pulpitis through targeting KIF‐5C and may serve as a potential therapeutic target.

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Section: Discussionmentioning

confidence: 99%

Overexpression of MicroRNA‐155 aggravates pulpitis by targeting kinesin superfamily Proteins‐5C based on illumina high‐throughput sequencing

Jiang

Chen

et al. 2023

Int Endodontic J

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“…In oral diseases and pathology, microRNAs are the most investigated small non-coding RNA with the highest potential to be utilized in clinical practice. MicroRNAs play a very important role in dental pulp pathology via their role in regulating the immune response and inflammation [ 46 ]. miR-21 was shown to mitigate the inflammatory signaling in LPS-stimulated dental pulp cells [ 47 ].…”

Section: Ncrnas and Oral Inflammatory Diseasesmentioning

confidence: 99%

Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review

Roganović

Petrović

2022

IJMS

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Non-coding RNAs (ncRNAs) represent a research hotspot by playing a key role in epigenetic and transcriptional regulation of diverse biological functions and due to their involvement in different diseases, including oral inflammatory diseases. Based on ncRNAs’ suitability for salivary biomarkers and their involvement in neuropathic pain and tissue regeneration signaling pathways, the present narrative review aims to highlight the potential clinical applications of ncRNAs in oral inflammatory diseases, with an emphasis on salivary diagnostics, regenerative dentistry, and precision medicine for neuropathic orofacial pain.

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“…Small, non-coding RNAs, namely miRNAs (18–24 nucleotides), play a vital role in regulating apoptosis by intrinsic and extrinsic pathways [ 25 ]. These molecules act post-translationally, targeting pro-and anti-apoptotic genes [ 25 , 26 , 27 ]. It was found that an overexpressed miR-761 might alleviate chronic inflammation and induce the remodelling of nasal mucosa in CRS mice.…”

Section: Introductionmentioning

confidence: 99%

Expression of Apoptosis-Related Biomarkers in Inflamed Nasal Sinus Epithelium of Patients with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP)—Evaluation at mRNA and miRNA Levels

et al. 2022

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In chronic upper respiratory tract diseases, increased cell proliferative activity is observed, which is coordinated by BCL-2 proteins and small non-coding RNAs. This study aimed to determine the expression of critical apoptosis markers at the mRNA and miRNA levels in patients with chronic rhinosinusitis with nasal polyps (CSRwNP). The study group consisted of ten patients with CSRwNP and ten healthy subjects. To detect in situ apoptosis in the maxillary sinus mucosa, TUNEL staining was performed. The expression of transcripts was determined by RT-qPCR and included the detection of markers associated with cell survival and apoptosis, i.e., BAX, p53, p21, CASP3, CASP9, c-MYC, CCND1, BRIC5, and APAF1. Levels of miR-17-5p, miR-145-5p, miR-146a-5p, and miR-203a-3p were also measured by RT-qPCR. The obtained results indicated increased apoptosis determined by a TUNEL assay in CSRwNP patients and accompanied by an increased expression of BAX, P21, P53, CASP3, CASP9, c-MYC, and APAF-1 transcripts and decreased mRNA levels of BCL-2 and BIRC5. Furthermore, the nasal sinus epithelium of patients with CSRwNP showed increased levels of miR-203a-3p while also showing a decreased expression of miR-17-5p and miR-145-5p. Our results showed that pro-apoptotic transcripts detected at mRNA and miRNA levels might be involved in the pathogenesis of chronic sinusitis with polyps. The identification of those key molecular mediators may be applicable for the specific diagnostic and/or development of targeted therapies for chronic sinusitis with polyps.

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The Role of microRNAs in Pulp Inflammation

Cited by 17 publications

References 175 publications

Overexpression of MicroRNA‐155 aggravates pulpitis by targeting kinesin superfamily Proteins‐5C based on illumina high‐throughput sequencing

Overexpression of MicroRNA‐155 aggravates pulpitis by targeting kinesin superfamily Proteins‐5C based on illumina high‐throughput sequencing

Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review

Expression of Apoptosis-Related Biomarkers in Inflamed Nasal Sinus Epithelium of Patients with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP)—Evaluation at mRNA and miRNA Levels

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