The role of microneedles for drug and vaccine delivery

Quinn, Helen L.; Kearney, Mary‐Carmel; Courtenay, Aaron J.; McCrudden, Maeliosa; Donnelly, Ryan F.

doi:10.1517/17425247.2014.938635

Cited by 142 publications

(98 citation statements)

References 90 publications

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“…Hollow microneedles have multiple benefits, for instance they can be used to inject a wide variety of fluids into the skin at different pressure-driven flow rates [3,5,6] and offer the highest precision in dose delivery among all microneedle types. Furthermore, they offer the possibility to screen formulations without time-consuming design and preparation of microneedles, as in case of coated and dissolving microneedles.…”

Section: Introductionmentioning

confidence: 99%

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

Groot

Mönkäre

et al. 2017

Journal of Controlled Release

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A B S T R A C TThe skin is an attractive organ for immunization due to the presence of a large number of epidermal and dermal antigen-presenting cells. Hollow microneedles allow for precise and non-invasive intradermal delivery of vaccines. In this study, ovalbumin (OVA)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles with and without TLR3 agonist poly(I:C) were prepared and administered intradermally by hollow microneedles. The capacity of the PLGA nanoparticles to induce a cytotoxic T cell response, contributing to protection against intracellular pathogens, was examined. We show that a single injection of OVA-loaded PLGA nanoparticles, compared to soluble OVA, primed both adoptively transferred antigen-specific naïve transgenic CD8 + and CD4 + T cells with markedly high efficiency. Applying a triple immunization protocol, PLGA nanoparticles primed also endogenous OVA-specific CD8 + T cells. Immune response, following immunization with in particular anionic PLGA nanoparticles co-encapsulated with OVA and poly(I:C), provided protection against a recombinant strain of the intracellular bacterium Listeria monocytogenes, secreting OVA. Taken together, we show that PLGA nanoparticle formulation is an excellent delivery system for protein antigen into the skin and that protective cellular immune responses can be induced using hollow microneedles for intradermal immunizations.

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Section: Introductionmentioning

confidence: 99%

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

Groot

Mönkäre

et al. 2017

Journal of Controlled Release

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“…In the past 10 years microneedles have been extensively investigated for delivery of drugs and vaccines by several groups [4–6] and more recently they are being explored as a diagnostic tool [7–9]. In the field of skin immunization microneedles have shown to present several immunological advantages when compared to conventional routes of delivery for vaccines against influenza [10, 6], Hepatitis B [11], malaria [12], polio [13], West Nile virus and Chikungunya [14] and a growing list of bacterial pathogens such as anthrax, diphtheria, tetanus, tuberculosis, botulism, plague, and staphylococcal toxin [15, 16].…”

Section: Introductionmentioning

confidence: 99%

Improved immunogenicity of individual influenza vaccine components delivered with a novel dissolving microneedle patch stable at room temperature

Vassilieva

Kalluri

McAllister

et al. 2015

Drug Deliv. and Transl. Res.

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Prevention of seasonal influenza epidemics and pandemics relies on widespread vaccination coverage to induce protective immunity. In addition to a good antigenic match with the circulating viruses, the effectiveness of individual strains represented in the trivalent vaccines depends on their immunogenicity. In this study we evaluated the immunogenicity of H1N1, H3N2 and B seasonal influenza virus vaccine strains delivered individually with a novel dissolving microneedle patch and the stability of this formulation during storage at 25°C. Our data demonstrate that all strains retained their antigenic activity after incorporation in the dissolving patches as measured by SRID assay and immune responses to vaccination in BALB/c mice. After a single immunization all three antigens delivered with microneedle patches induced superior neutralizing antibody titers compared to intramuscular immunization. Cutaneous antigen delivery was especially beneficial for the less immunogenic B strain. Mice immunized with dissolving microneedle patches encapsulating influenza A/Brisbane/59/07 (H1N1) vaccine were fully protected against lethal challenge by homologous mouse-adapted influenza virus. All vaccine components retained activity during storage at room temperature for at least three months as measured in vitro by SRID assay and in vivo by mouse immunization studies. Our data demonstrate that dissolving microneedle patches are a promising advance for influenza cutaneous vaccination due to improved immune responses using less immunogenic influenza antigens and enhanced stability.

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“…As MN-based research moves towards clinical use, more focus is required on the safety and efficacy of MN devices [58]. In order to have a delivery system capable of achieving regulatory approval, parameters such as reproducibly loading coated or dissolving MN arrays with DNA, consistently delivering the same quantity of DNA per MN application [56], demonstrating that cargo-loaded MNs are sufficiently robust to penetrate human skin [59] and the long-term stability of the DNA therapeutic, need to be investigated [45].…”

Section: Future Considerationsmentioning

confidence: 99%

Microneedles: an innovative platform for gene delivery

McCaffrey

Donnelly

McCarthy

2015

Drug Deliv. and Transl. Res.

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The advent of microneedle (MN) technology has provided a revolutionary platform for the delivery of therapeutic agents, particularly in the field of gene therapy. For over 20 years, the area of gene therapy has undergone intense innovation and progression which has seen advancement of the technology from an experimental concept to a widely acknowledged strategy for the treatment and prevention of numerous disease states. However, the true potential of gene therapy has yet to be achieved due to limitations in formulation and delivery technologies beyond parenteral injection of the DNA. Microneedle-mediated delivery provides a unique platform for the delivery of DNA therapeutics clinically. It provides a means to overcome the skin barriers to gene delivery and deposit the DNA directly into the dermal layers, a key site for delivery of therapeutics to treat a wide range of skin and cutaneous diseases. Additionally, the skin is a tissue rich in immune sentinels, an ideal target for the delivery of a DNA vaccine directly to the desired target cell populations. This review details the advancement of MN-mediated DNA delivery from proof-of-concept to the delivery of DNA encoding clinically relevant proteins and antigens and examines the key considerations for the improvement of the technology and progress into a clinically applicable delivery system.

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The role of microneedles for drug and vaccine delivery

Cited by 142 publications

References 90 publications

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

Improved immunogenicity of individual influenza vaccine components delivered with a novel dissolving microneedle patch stable at room temperature

Microneedles: an innovative platform for gene delivery

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