The role of metals in the enzymatic and nonenzymatic oxidation of epinephrine

Ryan, Timothy P.; Miller, Dennis M.; Aust, Steven D.

doi:10.1002/jbt.2570080106

Cited by 24 publications

(20 citation statements)

References 19 publications

(8 reference statements)

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“…After that, several other reports confirmed the pro-oxidant role of transition metals, either in their free form [355,357,358,428,430,450] or as cofactors in ferric transferrin or ferritin [451][452][453] and caeruloplasmin [454][455][456].…”

Section: The Oxidation Of Catecholaminesmentioning

confidence: 93%

Contribution of Catecholamine Reactive Intermediates and Oxidative Stress to the Pathologic Features of Heart Diseases

Costa

Carvalho

Bastos

et al. 2011

CMC

100

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Pathologic heart conditions, particularly heart failure (HF) and ischemia-reperfusion (I/R) injury, are characterized by sustained elevation of plasma and interstitial catecholamine levels, as well as by the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS). Despite the continuous and extensive research on catecholamines since the early years of the XX(th) century, the mechanisms underlying catecholamine-induced cardiotoxicity are still not fully elucidated. The role of catecholamines in HF, stress cardiomyopathy, I/R injury, ageing, stress, and pheochromocytoma will be thoroughly discussed. Furthermore and although the noxious effects resulting from catecholamine excess have traditionally been linked to adrenoceptors, in fact, several evidences indicate that oxidative stress and the oxidation of catecholamines can have important roles in catecholamine-induced cardiotoxicity. Accordingly, the reactive intermediates formed during catecholamine oxidation have been associated with cardiac toxicity, both in in vitro and in vivo studies. An insight into the influence of ROS, RNS, and catecholamine oxidation products on several heart diseases and their clinical course will be provided. In addition, the source and type of oxidant species formed in some heart pathologies will be referred. In this review a special focus will be given to the research of cardiac pathologies where catecholamines and oxidative stress are involved. An integrated vision of these matters is required and will be provided along this review, namely how the concomitant surge of catecholamines and ROS occurs and how they can be interconnected. The concomitant presence of these factors can elicit peculiar and not fully characterized responses on the heart. We will approach the existing data with new perspectives as they can help explaining several controversial results regarding cardiovascular diseases and the redox ability of catecholamines.

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Section: The Oxidation Of Catecholaminesmentioning

confidence: 93%

Contribution of Catecholamine Reactive Intermediates and Oxidative Stress to the Pathologic Features of Heart Diseases

Costa

Carvalho

Bastos

et al. 2011

CMC

100

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“…The buffers also contained 4 mmol/L DFO to reduce the rate of autoxidation of the EPI (Ryan et al. ). Muscles from eight mice were exposed to the DFO/EPI/NOREPI combination, with the other muscles being exposed to DFO control bath containing no catecholamines.…”

Section: Methodsmentioning

confidence: 99%

“…Muscles were exposed to a combination of L-Epinephrine (2 ng/mL) and L-Norepinephrine (10.5 ng/mL), corresponding to peak plasma levels seen in human exercise (Kr€ opfl et al 2014). The buffers also contained 4 mmol/L DFO to reduce the rate of autoxidation of the EPI (Ryan et al 1993). Muscles from eight mice were exposed to the DFO/EPI/NOREPI combination, with the other muscles being exposed to DFO control bath containing no catecholamines.…”

Section: Methodsmentioning

confidence: 99%

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Epinephrine stimulates CXCL 1 IL ‐1 α , IL ‐6 secretion in isolated mouse limb muscle

2017

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Catecholamines stimulate interleukin‐6 (IL‐6) secretion in skeletal muscles. However, whether other cytokines are secreted is currently unknown. Skeletal muscle ex vivo preparations commonly used to study cytokine secretion have dealt with limitations including auto‐oxidation of catecholamines. The use of metal chelators could be an alternative to avoid auto‐oxidation and allow catecholamines to be used at physiological doses. We exposed isolated soleus muscles to 1 or 100 ng/mL epinephrine (EPI) and collected bath samples at 1 and 2 h for multiplex cytokine analysis. Keratinocyte chemoattractant (CXCL1), IL‐6, and IL‐1α were significantly elevated by 100 ng/mL exposure, but not by 1 ng/mL (median [CXCL1] (2 h) = 83 pg/mL; [IL‐6] = 19 pg/mL; IL‐1α = 7.5 pg/mL). CXCL1 and IL‐6 were highly correlated in each sample (P = 0.0001). A second experiment combined the metal chelator, deferoxamine mesylate (DFO), to prevent EPI autoxidation, with 2 ng/mL EPI and 10.5 ng/mL norepinephrine (NOREPI) to mimic peak exercise. Unexpectedly, DFO alone stimulated both IL‐6 and CXCL1 secretion, but together with EPI and NOREPI had no additional effects. Stimulation of cytokine secretory responses from skeletal muscle cells in response to DFO thus precludes its use as a chelating agent in ex vivo models. In conclusion, 100 ng/mL EPI stimulates a robust secretory CXCL1 response, which together with IL‐6 and IL‐1α, may constitute an adrenal‐muscle endocrine response system.

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“…In 1991, Mordvintcev et al employed the great affinity of NO for transition metal ions to develop a method in which they used the complex of Fe e with DETC (diethylthiocarbamate) to trap NO and form a stable ternary complex (DETC)2-Fez -NO. This ternary complex was then detected by ESR spectroscopy at 77 K [22,23]. The spectral feature of the ternary complex (DETC)2-Fee -NO is an axial signal with an easily recognized hyperfine structure (hfs) triplet at g1 = 2.035.…”

Section: Introductionmentioning

confidence: 99%

Improved method to detect nitric oxide in biological systems

Zhang

Xiong

et al. 2001

Appl. Magn. Reson.

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In this work an improved method is described for using organic solvent extracting to detect nitric oxide. The partition coefficients of the diethylthiocarbamate (DETC)-Fe 2+ complex between different organic solvents and water, the signal intensity of the same NO trapping complex concentration in different organic solvents, and the extracting abilities of the organic solvents were determined. It was found that ethyl acetate was the optimal organic solvent. With ethyl acetate as extracting solvent, the (DETC) 2 -Fe"-NO complex was extracted from water phase to organic phase, and low concentration of nitric oxide in large volume could be detected by electron spin resonance (ESR) at room tem -p e r a t u r e . T h e E S R s i g n a l i n t e n s i t y h a d a g o o d l i n e a r r e l a t i o n s h i p w i t h t h e c o n c e n t r a t i o n o f n i t r i c o x i d e , at a signal-to-noise ratio of 2. The detection threshold of this method was improved to lower than 50 nM, which was more sensitive than the usually used method. The (DETC)z FeZ*-NO complex was stable in the dark at 0-4°C, and there was little change after days. Nitric oxide produced by cardiomyocytes cultured in media and other biological systems was firstly detected with this method.

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The role of metals in the enzymatic and nonenzymatic oxidation of epinephrine

Cited by 24 publications

References 19 publications

Contribution of Catecholamine Reactive Intermediates and Oxidative Stress to the Pathologic Features of Heart Diseases

Contribution of Catecholamine Reactive Intermediates and Oxidative Stress to the Pathologic Features of Heart Diseases

Epinephrine stimulates CXCL 1 IL ‐1 α , IL ‐6 secretion in isolated mouse limb muscle

Improved method to detect nitric oxide in biological systems

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