The role of mediating factors involved in angiogenesis during implantation

Boldeanu, Lidia; Dijmărescu, Anda Lorena; Radu, Mirela; Siloşi, Cristian Adrian; Popescu-Drigă, Mircea Vasile; Poenariu, Ioan Sabin; Siloşi, Isabela; Boldeanu, Mihail Virgil; Novac, Marius Bogdan; Novac, Liliana

doi:10.47162/rjme.61.3.04

Cited by 11 publications

(9 citation statements)

References 76 publications

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“…But we believe that the presence of syncytial knots, intravillous microcalcifications, and placental infarction over 5%, may cause the development of a IUGR and likely trigger PB. With these results, we are consistent with other studies [ 36 , 37 ]. With the help of PAS-H staining, we highlighted the intra/extravillous vascular basement membranes, but especially the massive fibrin deposits present, rich in glycosaminoglycans.…”

Section: Discussionsupporting

confidence: 94%

Analysis of the relationship between placental histopathological aspects of preterm and term birth

Camen

Istrate-Ofiţeru²,

Novac³

et al. 2022

Rom J Morphol Embryol

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Objectives: This study aims to establish a correlation between placental histopathological and immunohistochemical (IHC) changes and preterm birth with fetal growth restriction (FGR, formerly called intrauterine growth restriction -IUGR). Patients, Materials, and Methods: This prospective study was performed on a group of 30 parturients, with singleton gestation, of which 15 patients gave birth at term, and the other 15 patients gave birth prematurely. After the statistical correlation of the clinical and demographic data with premature birth (PB) and term birth (TB), we performed histological and IHC research on the respective placentae. To observe normal and pathological microscopic placental structures, we used the Hematoxylin-Eosin (HE) and Periodic Acid Schiff-Hematoxylin (PAS-H) classical stainings, but also special immunostaining with anti-cluster of differentiation 34 (CD34) and anti-vascular endothelial growth factor (VEGF) antibodies. Results: We found a statistically significant difference between the TB/PB categories and the age of the patients, their antepartum weight, the weight of the newborns, and the placenta according to the sex of the newborn. Histological analysis revealed in the case of TB, small areas of perivillous amyloid deposition, with the significant extension of these areas both intravillous and perivillous in the case of PB. Massive intravillous calcifications, syncytial knots, and intravillous vascular thrombosis were also frequently present in PB. With PAS-H staining were highlighted the intra/extravillous vascular basement membranes, but especially the massive fibrin deposits rich in glycosaminoglycans. By the IHC technique with the anti-CD34 antibody, we noticed the numerical vascular density, higher in the case of TB, but in the case of PB, there were large areas of placental infarction, with a lack of immunostaining in these areas. Through the anti-VEGF antibody, we observed the presence of signal proteins that determined and stimulated the formation of neoformation vessels in the areas affected by the lack of post-infarction placental vascularization. We observed a highly significant difference between placental vascular density between TB/PB and newborn weight, sex, or placental weight. Conclusions: Any direct proportional link between the clinical maternal-fetal and histological elements yet studied must be considered. Thus, establishing an antepartum risk group can prevent a poor pregnancy outcome.

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Section: Discussionsupporting

confidence: 94%

Analysis of the relationship between placental histopathological aspects of preterm and term birth

Camen

Istrate-Ofiţeru²,

Novac³

et al. 2022

Rom J Morphol Embryol

Self Cite

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“…Furthermore, the addition of these cytokines to the culture medium has increased the blastulation rate by stimulating cell division and survival rate after cryopreservation by enhancing the resistance to thermal shock ( Stoecklein et al, 2021 ). Their action seems to be synergistic because their combination in a culture medium improves the embryo number by increasing the cell number and embryo quality ( Boldeanu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Follicular Fluid Growth Factors and Interleukin Profiling as Potential Predictors of IVF Outcomes

et al. 2022

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Growth hormone (GH) has gained attention as an anti-aging compound enhancing oocyte quality. In fact, GH is known to activate intrafollicular metabolic events for oocyte maturation. Insulin growth factor I (IGF1) is another ovarian growth factor that mediates the FSH and GH actions. Cytokines could also increase IVF outcomes. Indeed, IL-6 is a pleiotropic cytokine with multiple cellular effects that can vary based on the physiological environment. IL-6 may also play an important role in follicular development (Yang et al., J Assist Reprod Genet, 2020, 37 (5), 1171–1176). Clinical studies have been performed to explore the potential role of IL-6 in human oocyte maturation and subsequent embryonic development. To date, the answers are not conclusive. During peri-implantation, many cytokines balances are regulated like pro-inflammatory and anti-inflammatory interleukins. The pro-inflammatory properties of IL-17 and its impact on the tumor microenvironment or autoimmune diseases are characterized, but new dimensions of IL-17 activity that promotes embryo implantation are not well explored. In the search for answers, our study compared concentrations of growth factors IGF1, GH, and interleukins IL-6 and IL-17 in the follicular fluid (FF) from 140 women divided into two groups depending on bad (G1) or good prognosis (G2) and investigated the relationships between these FF components’ levels and the main parameters of IVF. GH, IGF1, and IL-6 were significantly higher for G2. For GH, it was negatively correlated to patient age and positively correlated to maturity rate and IGF1. Moreover, GH and IGF1 were correlated to the top embryo rate and cumulative pregnancy rate. Regarding IL-6, it was correlated to IGF1 level, endometrium thickness, and implantation rate. As for IL-17, it was only correlated to IL-6. Consequently, all these FF components were predictive of oocyte quality except IL-17. GH seemed to be the best biomarker of this quality.

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“…The immune response is affected in anesthesia by inhibiting/releasing cytokines that can affect the inflammatory response, along with the body’s metabolic and immune response to surgical stress, influencing the profile of inflammatory factors and especially of cytokines [ 12 , 33 , 34 , 35 ].…”

Section: ⧉ Discussionmentioning

confidence: 99%

The perioperative effect of anesthetic drugs on the immune response in total intravenous anesthesia in patients undergoing minimally invasive gynecological surgery

Novac

Boldeanu²,

Rotaru³

et al. 2022

Rom J Morphol Embryol

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Background : The specific mechanism of action of each anesthetic drug on the immune system is still incompletely known. It is important to know how the various anesthetics used in minimally invasive surgery (MIS) act on the inflammatory response because the choice of the anesthetic agent can influence the patient’s immune system. Aim : Evaluation of the effect of anesthetic drugs used for total intravenous anesthesia (Propofol and Midazolam) on the inflammatory response after minimally invasive gynecological surgery. Patients, Materials and Methods : The inflammatory response in 20 female patients who underwent minimally invasive gynecological surgery under which intravenous anesthesia was performed. Depending on the combination of anesthetics used, we subdivided the study group into two groups, Group 1 consisting of the patients ( n =10) who were given for total intravenous anesthesia, the combination with Midazolam+Fentanyl, and Group 2 ( n =10) the patients who received the combination of Propofol+Fentanyl, respectively. Surgical interventional procedures included day surgery: diagnostic and operative hysteroscopy, endometrial ablation, surgical treatment of vulvar disorders. Serological profiling of patients was performed by dosing the serum concentration of nucleotide-binding domain (NOD) and leucine-rich repeat protein 3 (NLRP3) inflammasomes, interleukin (IL)-6, tumor necrosis factor-alpha (TNF- α ), IL-10 before and two hours after the surgical procedure. Results : In our study, we found that in both groups of patients (Midazolam+Fentanyl – Group 1, Propofol+Fentanyl – Group 2), NLRP3 and cytokines concentrations in the serum were higher after MIS than those before MIS. Conclusions : It appears that both Midazolam and Fentanyl and Propofol and Fentanyl have an immunomodulatory action due to the anti-inflammatory effect of both anesthetics. Therefore, anesthesiologists must choose an anesthetic method that uses individualized anesthetic agents, depending on the patient’s immune status and disease.

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The role of mediating factors involved in angiogenesis during implantation

Cited by 11 publications

References 76 publications

Analysis of the relationship between placental histopathological aspects of preterm and term birth

Analysis of the relationship between placental histopathological aspects of preterm and term birth

Follicular Fluid Growth Factors and Interleukin Profiling as Potential Predictors of IVF Outcomes

The perioperative effect of anesthetic drugs on the immune response in total intravenous anesthesia in patients undergoing minimally invasive gynecological surgery

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