The role of matrix metalloproteinases and cysteine-cathepsins on the progression of dentine erosion

Zarella, B.L.; Cardoso, Cristiane de Almeida Baldini; Pelá, Vinícius Taioqui; Kato, Melissa Thiemi; Tjäderhane, Leo

doi:10.1016/j.archoralbio.2015.06.011

Cited by 46 publications

(41 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 a) that can be degraded by endogenous enzymes, e.g. metalloproteases and cysteine cathepsins [Zarella et al, 2015]. Moreover, further collagen degradation can be also induced by salivary esterases and/or bacteria proteases [Park et al, 2008].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules

et al. 2016

View full text Add to dashboard Cite

The present investigation evaluated the ability of an experimental di-calcium phosphate (DCP) desensitising agent used alone or combined with phytosphingosine (PHS) to occlude dentine tubules and resist a citric acid (CA) or artificial saliva (AS) challenge. Three groups of human dentine specimens (DS) were treated with the following: (1) PHS alone, (2) DCP or (3) a combination of PHS and DCP. Dentine hydraulic conductance of DS was evaluated using a digital flow sensor at 6.9 kPa. The average fluid volume for each of the treated DS was used to calculate the total dentine permeability reduction (%P) prior to and following CA immersion for 1 min or AS immersion for 4 weeks. The treated DS were subjected to both scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy analysis. Statistically significant differences (%P) were identified between the groups by ANOVA and Fisher's multiple comparison test (p < 0.05), respectively. Interestingly, both PHS and DCP appeared to work synergistically. DS treated with DCP or PHS/DCP demonstrated a significant reduction (%P) prior to and following CA or AS challenge (p < 0.05). Both the SEM and FTIR analyses showed consistent brushite crystals occluding the dentine tubules. Conversely, the application of PHS alone failed to demonstrate any significant reduction of dentine permeability (p > 0.05) or show any evidence of occlusion of the dentine tubules. DCP can be used alone or combined with PHS to decrease the dentine permeability as well as to resist a CA and AS challenge. These results would, therefore, suggest that DCP may be a suitable treatment option for dentine hypersensitivity.

show abstract

Section: Discussionmentioning

confidence: 99%

“…calcium phosphates and bioactive glasses may also reduce the enzymatic-mediated collagen degradation [Tezvergil-Mutluay et al, 2014] and the risk of any further wear of hard tissues (e.g. dentine) [Zarella et al, 2015].…”

Section: Discussionmentioning

confidence: 99%

Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules

et al. 2016

View full text Add to dashboard Cite

show abstract

“…However, it can be degraded by the action of proteases such as pepsin in saliva of bulimic patients [Schlueter et al, 2011] or matrix metalloproteinases (MMPs) and perhaps cathepsins constitutively present in saliva and dentin [Zarella et al, 2015], which might speed up the progression of tooth erosive loss. It is expected that, clinically, erosive lesions have active degradation of the DOM by the action of proteases.…”

mentioning

confidence: 99%

The Impact of the Demineralized Organic Matrix on the Effect of TiF<sub>4</sub> Varnish on the Progression of Dentin Erosive Loss

Souza

Vertuan²,

Buzalaf³

et al. 2017

Caries Res

View full text Add to dashboard Cite

This in vitro study compared the effect of TiF₄ varnish with that of NaF varnish, applied on pre-eroded bovine dentin samples, with respect to the progression of erosive loss, in the presence or absence of the demineralized organic matrix (DOM). One hundred and sixty bovine dentin samples were pre-eroded (0.1% citric acid, pH 2.5, 30 min). Half of the samples were subjected to the DOM removal (collagenase solution, 5 days). Samples with and without the DOM were treated according to the groups (n = 20 with DOM and 20 without DOM/group): TiF₄ varnish (2.45% F), NaF varnish (2.45% F), placebo varnish (without fluoride) and control (no treatment). Thereafter, the treated samples were submitted to erosive challenges 4 × 90 s/day (0.1% citric acid, pH 2.5) during 7 days. Between the challenges, the samples were immersed in artificial saliva. The dentin erosive loss was measured using contact profilometry (µm, n = 15). Five dentin samples per group were prepared for energy-dispersive X-ray spectroscopy analysis. Data were compared using 2-way ANOVA/Bonferroni test (p < 0.05). Both fluoride varnishes were effective in reducing the erosive loss progression regardless of the dentin condition when compared to placebo varnish and control groups. Despite the fact that the TiF₄ varnish was more effective than the NaF varnish for both dentin conditions (p < 0.001), its effect was significantly reduced in the absence of DOM (p < 0.05). It can be concluded that the TiF₄ varnish is the best treatment in reducing the progression of dentin erosive loss (100%) in vitro, but its protective effect is more pronounced in the presence of DOM.

show abstract

“…Recent literature [Kato et al, 2009;Magalhães et al, 2009;Kato et al, 2010;Zarella et al, 2015;De Moraes et al, 2016] about the role of these endogenous enzymes in the progression of dental erosion has focused on the use of MMP inhibitors in order to prevent this type of lesion. These studies evaluated the final effects of different MMP inhibitors regarding the loss of dentin, using indirect methods of evaluation such as profilometry.…”

mentioning

confidence: 99%

“…Only recently, some researchers [Buzalaf et al, 2012;Zarella et al, 2015], have focused on the investigation of the role of these enzymes in dental erosion. In this context, the few available studies consider that the process of organic matrix degradation is similar to that found for tooth decay Buzalaf et al, 2012].…”

mentioning

confidence: 99%

Dynamic Influence of pH on Metalloproteinase Activity in Human Coronal and Radicular Dentin

Amaral

Scaffa²,

Rodrigues³

et al. 2018

Caries Res

View full text Add to dashboard Cite

The aim of this study was to evaluate the effect of pH on the activation of matrix metalloproteinases (MMPs) of human coronal (CD) and radicular dentin (RD). CD and RD were pulverized to powder, and proteins were extracted with 1% phosphoric acid. The extracted proteins and the demineralized powder were separately incubated in the following solutions: 4-aminophenylmercuric acetate (control) or a buffer solution at different pHs (2.5, 4.5, 5.0, 6.0, and 7.0). After incubation, proteins were separated by electrophoresis to measure MMP activities by zymography. To assess the solubilized dentin collagen, the demineralized dentin powder was sustained in incubation buffer, and the amount of hydroxyproline (HYP) released was measured. Zymography revealed MMP-2 gelatinolytic activities for CD and RD in all experimental groups. For both substrates, the lowest pH solutions (2.5, 4.5, and 5.0) yielded higher gelatinolytic activity than those obtained by the highest pH solutions (6.0 and 7.0). For HYP analysis, no detectable absorbance values were observed for pHs of 2.5 and 4.5. The amount of HYP was higher for pH 7.0 than those of all other groups (p < 0.05), except for pH 6.0. No statistical differences were found between pHs 6.0 and 5.0 and control (p > 0.05). The MMP-2 enzyme from human CD and RD is dynamically influenced by pH: at low pH, the extracted enzyme activates this latent form, whereas collagen degradation by the matrix-bound enzyme is only observed when pHs are close to neutral.

show abstract

The role of matrix metalloproteinases and cysteine-cathepsins on the progression of dentine erosion

Cited by 46 publications

References 31 publications

Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules

Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules

The Impact of the Demineralized Organic Matrix on the Effect of TiF<sub>4</sub> Varnish on the Progression of Dentin Erosive Loss

Dynamic Influence of pH on Metalloproteinase Activity in Human Coronal and Radicular Dentin

Contact Info

Product

Resources

About