Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules

Abstract

The present investigation evaluated the ability of an experimental di-calcium phosphate (DCP) desensitising agent used alone or combined with phytosphingosine (PHS) to occlude dentine tubules and resist a citric acid (CA) or artificial saliva (AS) challenge. Three groups of human dentine specimens (DS) were treated with the following: (1) PHS alone, (2) DCP or (3) a combination of PHS and DCP. Dentine hydraulic conductance of DS was evaluated using a digital flow sensor at 6.9 kPa. The average fluid volume for each of the treated DS was used to calculate the total dentine permeability reduction (%P) prior to and following CA immersion for 1 min or AS immersion for 4 weeks. The treated DS were subjected to both scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy analysis. Statistically significant differences (%P) were identified between the groups by ANOVA and Fisher's multiple comparison test (p < 0.05), respectively. Interestingly, both PHS and DCP appeared to work synergistically. DS treated with DCP or PHS/DCP demonstrated a significant reduction (%P) prior to and following CA or AS challenge (p < 0.05). Both the SEM and FTIR analyses showed consistent brushite crystals occluding the dentine tubules. Conversely, the application of PHS alone failed to demonstrate any significant reduction of dentine permeability (p > 0.05) or show any evidence of occlusion of the dentine tubules. DCP can be used alone or combined with PHS to decrease the dentine permeability as well as to resist a CA and AS challenge. These results would, therefore, suggest that DCP may be a suitable treatment option for dentine hypersensitivity.

Improved reactive nanoparticles to treat dentin hypersensitivity

“…Other effective compounds as calcium sodium phosphosilicates form hydroxycarbonate apatite [4] which showed high rates of solubility [7]. Calcium, potassium, and ferric oxalate have also been used to treat DH, but oxalate precipitates were removed or dissolved by saliva [27]. Nano-hydroxyapatite, which has been recently recommended for tubular occlusion, forms soluble compounds that impair its long term efficacy [34].…”

“…Specimens were connected to a hydraulic pressure device with a liquid flow sensor (ASL 1600, Sensirion, Staefa, Switzerland). A constant hydraulic pressure of 6.9 kPa was applied [26] to simulate physiological pulp pressure [26,27] (Fig 1a).…”

Zinc and silica are active components to efficiently treat in vitro simulated eroded dentin

“…Specimens were connected to a hydraulic pressure device with a liquid flow sensor (ASL 1600, Sensirion, Staefa, Switzerland). A constant hydraulic pressure of 6.9 kPa was applied [12]. The measurement of the fluid volume through the sensor was performed.…”

“…Most of the previously published studies [4,7,9,10,11,12] evaluate the efficacy of these remineralizing products by examining the degree of tubule occlusion conferred by the desensitizing materials. These reports are far to elucidate the quality of the formed mineralized layer and to distinguish it from a weak smear layer due to dentin abrasion.…”

Avaliação da estabilidade da aplicação de um híbrido experimental em diferentes concentrações sobre a dentina sensível - in vitro

“…Conclui-se que o DCP pode ser um tratamento desejado para HD(65).O ionômero de vidro, ionômero de vidro reforçado com resina e adesivos resinosos, vem sendo utilizados com sucesso para o tratamento da HD. Este tratamento pode ser problemático se a perda de tecido na região for pequena(25).…”