Improved reactive nanoparticles to treat dentin hypersensitivity

Toledano, Manuel; Osorio, Estrella; Aguilera, Fátima S.; Medina-Castillo, Antonio L.; Osorio, Raquel

doi:10.1016/j.actbio.2018.03.033

Cited by 56 publications

(63 citation statements)

References 36 publications

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“…The tooth was cut perpendicular to the axial axis using a diamond saw (Accutom-50 Struers, Copenhagen, Denmark) under copious water irrigation to obtain cementodentinal slabs with a thickness of 1 mm. Specimens were then prepared as in Toledano-Osorio et al , 2018 (11). A phosphate buffered suspension (PBS) of NPs (10 mg/ml), Ag-NPs (10 mg/ml) or just a PBS solution were applied (30 s), in each of the three different experimental groups.…”

Section: Methodsmentioning

confidence: 99%

Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin

Toledano¹,

Aguilera²,

Cabello³

et al. 2019

Med Oral

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Background The aim was to evaluate the effect of silver loaded nanoparticles (NPs) application on the triboscopic, crystallographic and viscoelastic properties of demineralized dentin. Polymethylmetacrylate-based NPs and Ag loaded NPs were applied on demineralized dentin. Material and Methods Treated and untreated surfaces were probed by a nanoindenter to test viscoelasticity, and by atomic force microscopy to test nanoroughness and collagen fibril diameter. X-ray diffraction and transmission electron microscopy through selected area diffraction and bright-field imaging were also used. Results Dentin treated with Ag-NPs attained the lowest complex modulus, and the highest tan delta values after 7 days of storage. Dentin treated with undoped-NPs achieved the lowest nanoroughness and the greatest collagen bandwidths among groups. Crystals were identified as hydroxyapatite with the highest crystallographic maturity and crystallite size in dentin treated with undoped-NPs. Texture increased in all samples from 24 h to 7 d, except in dentin surfaces treated with Ag-NPs at 310 plane. Polyhedral, block-like, hexagonal or plate-like shaped apatite crystals constituted the bulk of minerals in dentin treated with Ag-NPs, after 7 d. Polyhedral or rounded/drop-like, and polymorphic in strata crystal apatite characterized the minerals when undoped-NPs were used, with more crystalline characteristics after 7 d than that found when Ag-NPs were applied. Ag-NPs application did not improve the mechanical performance of dentin and did not produce dentin remineralization. However, energy was dissipated through the dentin without showing stress concentration; contrary was occurring at dentin treated with undoped-NPs, that provoked bridge-like mineral deposits at the dentin surface. Conclusions Ag-NPs application did not enhance the mechanical properties of cervical dentin, though the energy dissipation did not damage the dentin structure. Remineralization at dentin was not produced after Ag-NPs application, though improved crystallinity may lead to increase stability of the apatite that was generated at the dentin surface. Key words: Dentin, mechanical, mineralization, roughness, silver, viscoelastic.

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Section: Methodsmentioning

confidence: 99%

Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin

Toledano¹,

Aguilera²,

Cabello³

et al. 2019

Med Oral

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“…(1) This hypersensitivity can cause difficulty in eating, drinking, and oral hygiene maintenance due to the high level of irritation. (2) The hydrodynamic theory is the most accepted theory that explains the cause of this sensitivity. According to this theory, environmental stimuli cause changes in the flow of tubular fluid.…”

Section: Original Article Introductionmentioning

confidence: 99%

Evaluation of the Prevalence of Dentin Hypersensitivity and Associated Factors: A Cross-Sectional Study

Mahdisiar

Anaraki

Bineshian³

et al. 2019

J Res Dentomaxillofac Sci

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Background and Aim: Dentin hypersensitivity (DH) is one of the most common problems in dentistry. Several factors affect DH. This study aimed to determine the prevalence of this problem and identify the associated factors. Materials and Methods: : In this cross-sectional study, 300 patients who referred to the operative dentistry department of Islamic Azad University of Medical Sciences, Tehran, Iran, were examined. Demographic information and other related factors were recorded using a questionnaire. One examiner then examined the subjects regarding the potential related clinical factors. DH was evaluated using evaporative and tactile tests and was categorized using the visual analog scale (VAS). Descriptive data were presented as frequency and mean. Chi-Square test was used to examine the related factors at the significance level of 0.05. Result: The overall prevalence of DH was 21%. The correlation of horizontal brushing technique (P<0.005), hard toothbrushes (P<0.0001), gingival recession (P<0.0001), history of gingival surgery (P<0.001), tooth wear (P<0.001), traumatic occlusion (P<0.005), and bleaching toothpastes (P<0.005) with DH prevalence was significant. Conclusion: According to the results, high awareness about DH and its associated factors is mandatory.

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“…NP sizes were not altered after loading, and there was no NP agglomeration. Polydispersity index of NPs was 0.05 ± 0.008 [39]. To proceed with the experiments, (1) undoped NPs (NPs), (2) (D-NPs), and (3) Zn-doped NPs (Zn-NPs) were suspended in phosphate buffered saline solution and tested at different concentrations.…”

Section: Methodsmentioning

confidence: 99%

Novel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis elegans

et al. 2019

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The objective was to evaluate the toxicity of zinc- and doxycycline-loaded polymeric nanoparticles (NPs) using Caenorhabditis elegans as a model organism. These NPs are composed of ethylene glycol dimethacrylate, 2-hydroxyethyl methacrylate and methacrylic acid. NPs were loaded with doxycycline (D-NPs) and zinc (Zn-NPs) by chemical adsorption, and loading efficacy was demonstrated. Worm death rate in a concentration-response curve basis was calculated for lethality. Metabolism was evaluated through pharyngeal pumping assay. Body length measurements, brood size and egg lays were used to gauge growth, reproduction and fertility respectively. Intracellular hydrogen peroxide levels were determined to assess the reactive oxygen species production. One-way ANOVA and Bonferroni were used for comparisons (p < 0.05). Tested NPs at the highest dosage did not affect lethality or worm metabolism, expressed in terms of death rate and pharyngeal pumping per minute, respectively. Zn-NPs slightly increased worm growth. The concentration of the intracellular hydrogen peroxide levels was the lowest in the D-NPs group. The distinct NPs and concentrations employed were shown to be non-toxic for in situ administration of zinc and doxycycline, reducing the harmful effects of these compounds.

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Improved reactive nanoparticles to treat dentin hypersensitivity

Cited by 56 publications

References 36 publications

Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin

Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin

Evaluation of the Prevalence of Dentin Hypersensitivity and Associated Factors: A Cross-Sectional Study

Novel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis elegans

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