The role of matrix metalloproteinases in the oral environment

Hannas, Angélica Reis; Pereira, José Carlos; Granjeiro, José Mauro; Tjäderhane, Leo

doi:10.1080/00016350600963640

Cited by 327 publications

(367 citation statements)

References 98 publications

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“…The gels were stained with Coomassie Blue G-250 (0.5%), scanned using the Imagemaster scanner (GE Healthcare ® , Uppsala, Sweden) followed by Kodak Molecular Imaging Software (Kodak Co., Rochester, NY, USA) for analysis. The results show the mean of 3 independent gels, as reported by Hannas et al (14).…”

Section: Zymography Gel Analysismentioning

confidence: 90%

Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

et al. 2012

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This study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10 -6 M, 10 -5 M, 10 -4 M and 10 -3 M) for 24, 48, 72 and 96 h at 37 o C, 5% CO 2 . Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10 -3 M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling.

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Section: Zymography Gel Analysismentioning

confidence: 90%

Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

et al. 2012

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“…Although the physiological roles of MMPs in dentin are not well understood, they have been suggested to participate in peritubular and tertiary dentin formation and in the release of dentinal growth factors Hannas et al 2007;Charadram et al 2012). Unlike bone, mineralized dentin does not undergo significant restructuring; nevertheless, predentin and nonmineralized dentin inside the dentinal tubules seem to be modified in response to the functional demands.…”

mentioning

confidence: 99%

“…They are usually expressed as inactive zymogens, and the prodomain must be dissociated from the catalytic one for its activation (Hannas et al 2007). In nonactivated MMPs, the unpaired cysteine in the prodomain forms a bridge with the catalytic zinc (referred to as the "cysteine switch" mechanism), preventing enzymatic activity and acting as a ligand for the catalytic zinc atom in the active site, excluding water molecules and rendering the enzyme inactive (Tjäderhane et al 2013a).…”

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confidence: 99%

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

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Dentin can be described as a biological composite with collagen matrix embedded with nanosized hydroxyapatite mineral crystallites. Matrix metalloproteinases (MMPs) and cysteine cathepsins are families of endopeptidases. Enzymes of both families are present in dentin and collectively capable of degrading virtually all extracellular matrix components. This review describes these enzymes and their presence in dentin, mainly focusing on their role in dentin caries pathogenesis and loss of collagen in the adhesive hybrid layer under composite restorations. MMPs and cysteine cathepsins present in saliva, mineralized dentin, and/or dentinal fluid may affect the dentin caries process at the early phases of demineralization. Changes in collagen and noncollagenous protein structure may participate in observed decreases in mechanical properties of caries-affected dentin and reduce the ability of caries-affected dentin to remineralize. These endogenous enzymes also remain entrapped within the hybrid layer during the resin infiltration process, and the acidic bonding agents themselves (irrespective of whether they are etch-and-rinse or self-etch) can activate these endogenous protease proforms. Since resin impregnation is frequently incomplete, denuded collagen matrices associated with free water (which serves as a collagen cleavage reagent for these endogenous hydrolase enzymes) can be enzymatically disrupted, finally contributing to the degradation of the hybrid layer. There are multiple in vitro and in vivo reports showing that the longevity of the adhesive interface is increased when nonspecific enzyme-inhibiting strategies are used. Different chemicals (i.e., chlorhexidine, galardin, and benzalkonium chloride) or collagen cross-linker agents have been successfully employed as therapeutic primers in the bonding procedure. In addition, the incorporation of enzyme inhibitors (i.e., quaternary ammonium methacrylates) into the resin blends has been recently promoted. This review will describe MMP functions in caries and hybrid layer degradation and explore the potential therapeutic role of MMP inhibitors for the development of improved intervention strategies for MMP-related oral diseases.

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“…Two recent reviews summarized the role of MMPs in the oral environment and especially in the pathogenesis of dental caries 7,8 . Thus, host-derived MMPs localized in saliva and dentin could be responsible for the matrix degradation in dentinal caries lesions.…”

Section: Introductionmentioning

confidence: 99%

Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

Magalhães

Wiegand

Ríos

et al. 2009

Journal of Dentistry

126

119

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OBJECTIVES: This in situ/ex vivo study aimed to analyse the impact of possible MMPinhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. METHODS: Twelve volunteers took part in this cross-over and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoride-free toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60s with: 250 ppm F solution (SnF(2)/AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24, 100% Camellia Sinensis leaf extract, catechin concentration: 30+/-3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (microm). The data were analysed by two-way repeated measures ANOVA and Bonferroni post hoc test. RESULTS: There was a significant difference between the conditions (EroxEro+Abr, p<0.001) and among the solutions (p<0.001). All solutions (F: 1.42+/-0.34; 1.73+/-0.50, chlorhexidine: 1.15+/-0.26; 1.59+/-0.32, green tea: 1.06+/-0.30; 1.54+/-0.55) significantly reduced the dentin wear when compared to control (2.00+/-0.55; 2.41+/-0.83) for both conditions. There were not significant differences among green tea extract, chlorhexidine and F solutions. CONCLUSIONS: Thus, the possible MMP-inhibitors tested in this study seem to be a promising preventive measure to reduce dentin erosion-abrasion, but their mechanism of action needs to be investigated in further studies. Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. 2Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ ABSTRACT Objectives: This in situ/ex vivo study aimed to analyse the impact of possible MMP-inhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. Methods:Twelve volunteers took part in this crossover and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoridefree toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60 s with: 250 ppm F solution (SnF 2 /AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24 ® , 100% Camellia Sinensis leaf extract, catechin concentration:30±3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (µm). The data were analysed by two-way repeated m...

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The role of matrix metalloproteinases in the oral environment

Cited by 327 publications

References 98 publications

Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

Role of Dentin MMPs in Caries Progression and Bond Stability

Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

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