Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

Abstract

OBJECTIVES: This in situ/ex vivo study aimed to analyse the impact of possible MMPinhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. METHODS: Twelve volunteers took part in this cross-over and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoride-free toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60s with: 250 ppm F solution (SnF(2)/AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24, 100% Camellia Sinensis leaf extract, catechin concentration: 30+/-3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (microm). The data were analysed by two-way repeated measures ANOVA and Bonferroni post hoc test. RESULTS: There was a significant difference between the conditions (EroxEro+Abr, p<0.001) and among the solutions (p<0.001). All solutions (F: 1.42+/-0.34; 1.73+/-0.50, chlorhexidine: 1.15+/-0.26; 1.59+/-0.32, green tea: 1.06+/-0.30; 1.54+/-0.55) significantly reduced the dentin wear when compared to control (2.00+/-0.55; 2.41+/-0.83) for both conditions. There were not significant differences among green tea extract, chlorhexidine and F solutions. CONCLUSIONS: Thus, the possible MMP-inhibitors tested in this study seem to be a promising preventive measure to reduce dentin erosion-abrasion, but their mechanism of action needs to be investigated in further studies. Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. 2Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ ABSTRACT Objectives: This in situ/ex vivo study aimed to analyse the impact of possible MMP-inhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. Methods:Twelve volunteers took part in this crossover and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoridefree toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60 s with: 250 ppm F solution (SnF 2 /AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24 ® , 100% Camellia Sinensis leaf extract, catechin concentration:30±3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (µm). The data were analysed by two-way repeated m...

Evaluation of the pH and titratable acidity of teas commercially available in Brazilian market

“…The literature has reported many different properties of this tea against dental caries due to presence of their catechins [32][33] . Furthermore, green tea has components which can inhibit matrix metalloproteinase that are present on saliva and on mineralized dental tissues 34 decreasing the dental erosion/ abrasion in situ 35 .…”

Bonding efficiency and durability: current possibilities

“…Inhibitors of endogenous dentin proteases other than CHX, such as the quaternary ammonium surface-acting benzalkonium chloride (BAC) 124,125,126,127 the tetracyclines (TCs) and their antimicrobially inactive analogs (minocycline, for now), 71,84,128,129,130 bisphosphonates (batimastat, galardin, and zoled ron ate), 93,129,131,132 g reen tea poly phenol epigallocatechin-3-gallate (EGCG), 40,133,134 and the chelating agent ethylenediaminetetraacetic acid (EDTA) 135,136,137 are being studied as better alternatives associated with dentin hybrid layer preservation.…”

Effect of Green Tea Varnish on Depth of Root Caries

“…Previous studies on the properties of this material and its efficacy for prevention of tooth erosion are limited. Kato et al 47 and Magalhães et al 48 evaluated bovine teeth and concluded that green tea extract can effectively prevent dental caries. Similarly, the present study showed that there is a significant difference between the control and green tea groups in terms of depth of carious lesions.…”