The role of Malassezia furfur in dermatology

Ljubojević, Suzana; Skerlev, Mihael; Lipozenčić, Jasna; Basta-Juzbašić, Aleksandra

doi:10.1016/s0738-081x(01)00240-1

Cited by 35 publications

(32 citation statements)

References 25 publications

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“…Malassezia furfur, described as common in normal skin microbiota (37), has been associated with psoriasis (3). Using universal fungal primers, only 3 (1.5%) of 190 clones had sequences Ն97% similar to M. furfur (Table 1) and analysis using Malassezia-specific primers showed only 1 clone (0.07%) of the 1,374 analyzed.…”

Section: Discussionmentioning

confidence: 99%

Molecular Analysis of Fungal Microbiota in Samples from Healthy Human Skin and Psoriatic Lesions

Paulino¹,

Tseng²,

2006

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Psoriasis, a common cutaneous disease of unknown etiology, may be triggered by infections, including those due to fungi. Since the fungal community of human skin is poorly characterized, we aimed to analyze the mycological microbiota in healthy skin and psoriatic lesions. Twenty-five skin samples from five healthy subjects (flexor forearm) and three patients with psoriasis were analyzed using broad-range 18S ribosomal DNA (rDNA) and 5.8S rDNA/internal transcribed spacer 2 (ITS2) Malassezia-specific PCR primers. Broadrange PCR analysis indicated that most organisms resembled Malassezia. Malassezia-specific 5.8S/ITS2 analysis of 1,374 clones identified five species and four unknown phylotypes, potentially representing new species. The species distribution appears largely host specific and conserved in different sites of healthy skin. In three subjects, the Malassezia microbiota composition appeared relatively stable over time. Samples of Malassezia microbiota from healthy skin and psoriatic lesions were similar in one patient but substantially different in two others. These data indicate the predominance of Malassezia organisms in healthy human skin, host-specific variation, stability over time, and as yet, no consistent patterns differentiating psoriatic skin from healthy skin.Human skin is colonized by diverse microbiota, including bacteria and fungi, that can be pathogenic under particular circumstances (14, 16). Traditionally, microorganisms have been identified by culture-dependent methods; however, many species are fastidious and underrepresented in cultures from mixed microbial communities (13), whereas others cannot be cultivated under known conditions (2). Therefore, culture-independent molecular techniques have been used for the identification of microbial species within ecosystems (2, 9, 27, 42).Such methods, particularly the analysis of rRNA genes, have been employed to characterize bacterial and fungal communities associated with diverse human body sites, including intestine (11), gingiva (28,33,43), esophagus (45), vagina (65), and outer ear canal (13). As predicted, these studies revealed greater diversity, including previously undescribed organisms, than did previous analyses based on culture-dependent techniques.The application of molecular techniques has been advocated to characterize the microbiota in both healthy and diseased skin (14). To date, rRNA data have been used to identify species associated with fungal dermatoses (21,29,38,39) and PCR-based diagnostic tests have been developed (15,26,62). Psoriasis, a common dermatosis affecting about 3% of the population in industrialized countries (3), is characterized by erythrosquamous cutaneous lesions associated with abnormal patterns of keratinocyte growth and differentiation (35). Although of unknown etiology, trigger factors, including physical trauma and streptococcal infections, may provoke clinical manifestations (51). Fungal organisms, including Candida albicans (63) and Malassezia furfur (3), have also been associated with the developmen...

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Section: Discussionmentioning

confidence: 99%

Molecular Analysis of Fungal Microbiota in Samples from Healthy Human Skin and Psoriatic Lesions

Paulino¹,

Tseng²,

2006

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“…Their central role as the aggravating factor of atopic dermatitis has become the center of attention for the past several years and the number of research articles and case reports on this has shown a rapid increase [3][4][5][6] . However, despite a high level of interest, there have been only scarce studies on the inter-relationship between Malassezia and Malassezia folliculits to this point.…”

Section: Introductionmentioning

confidence: 99%

Epidemiologic Study ofMalasseziaYeasts in Patients withMalasseziaFolliculitis by 26S rDNA PCR-RFLP Analysis

Lee

Choe

et al. 2011

Ann Dermatol

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Background:So far, studies on the inter-relationship between Malassezia and Malassezia folliculitis have been rather scarce. Objective: We sought to analyze the differences in body sites, gender and age groups, and to determine whether there is a relationship between certain types of Malassezia species and Malassezia folliculitis. Methods: Specimens were taken from the forehead, cheek and chest of 60 patients with Malassezia folliculitis and from the normal skin of 60 age-and gender-matched healthy controls by 26S rDNA PCR-RFLP. Results: M. restricta was dominant in the patients with Malassezia folliculitis (20.6%), while M. globosa was the most common species (26.7%) in the controls. The rate of identification was the highest in the teens for the patient group, whereas it was the highest in the thirties for the control group. M. globosa was the most predominant species on the chest with 13 cases (21.7%), and M. restricta was the most commonly identified species, with 17 (28.3%) and 12 (20%) cases on the forehead and cheek, respectively, for the patient group. Conclusion: Statistically significant differences were observed between the patient and control groups for the people in their teens and twenties, and in terms of the body site, on the forehead only. (Ann Dermatol 23(2) 177∼184, 2011)

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“…The colony-formation begins immediately after the birth, and the population density increases on skin areas that have a high sebum production. Its prevalence is the highest for patients who are in late adolescence and early adulthood 2 . Malassezia yeast was first reported on in 1889, and it has been implicated in various diseases, such as pityriasis versicolor, seborrheic dermatitis and Malassezia folliculitis.…”

Section: Introductionmentioning

confidence: 99%

Molecular Analysis of Malassezia Microflora on the Skin of the Patients with Atopic Dermatitis

Yim

Kim

et al. 2010

Ann Dermatol

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Background:The yeasts of the genus Malassezia are members of the normal flora on human skin and they are found in 75∼80% of healthy adults. Since its association with various skin disorders have been known, there have been a growing number of reports that have implicated Malassezia yeast in atopic dermatitis (AD). Objective: The aim of the present study is to isolate the various Malassezia species from AD patients by using 26S rDNA (ribosomal Deoxyribonucleic acid) PCR-RFLP and to investigate the relationship between a positive Malassezia culture and the severity of AD. Methods: Cultures for Malassezia yeasts were taken from the scalp, cheek, chest, arm and thigh of 60 patients with atopic dermatitis. We used a rapid and accurate molecular biological method 26S rDNA PCR-RFLP, and this method can overcome the limits of the morphological and biochemical methods. Results: Positive Malassezia growth was noted on 51.7% of the patients with atopic dermatitis by 26S rDNA PCR-RFLP analysis. The overall dominant species was M. sympodialis (16.3%). M. restricta was the most common species on the scalp (30.0%) and cheek (16.7%). M. sympodialis (28.3%) was the most common species on the chest. The positive culture rate was the highest for the 11 ∼20 age group (59.0%) and the scalp showed the highest rate at 66.7%. There was no significant relationship between the Malassezia species and SCORing for Atopic Dermatitis (SCORAD). Conclusion: The fact that the cultured species was different for the atopic dermatitis lesion skin from that of the normal skin may be due to the disrupted skin barrier function and sensitization of the organism induced by scratching in the AD lesion-skin. But there was no relationship between the Malassezia type and the severity score. The severity score is thought to depend not on the type, but also on the quantity of the yeast.

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The role of Malassezia furfur in dermatology

Cited by 35 publications

References 25 publications

Molecular Analysis of Fungal Microbiota in Samples from Healthy Human Skin and Psoriatic Lesions

Molecular Analysis of Fungal Microbiota in Samples from Healthy Human Skin and Psoriatic Lesions

Epidemiologic Study ofMalasseziaYeasts in Patients withMalasseziaFolliculitis by 26S rDNA PCR-RFLP Analysis

Molecular Analysis of Malassezia Microflora on the Skin of the Patients with Atopic Dermatitis

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