The role of macronuclear DNA sequences in the permanent rescue of a non-mendelian mutation in Paramecium tetraurelia.

You, Yun; Scott, J M; Forney, James D.

doi:10.1093/genetics/136.4.1319

Cited by 42 publications

(3 citation statements)

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“…This 78% rescue efficiency is not unusual for the pSAlOXS A-gene plasmid. Similar rescue efficiencies have been obtained for microinjection of pSAIOXS into the d48 cell line (24). None of the nine cell lines transformed with pSAIOXS, however, contained the 2.1-kb Hindlll fragment of the B gene following autogamy.…”

Section: Resultssupporting

confidence: 68%

“…This was demonstrated by the ability of the cloned A gene to rescue the A but not the B deletion after formation of a new macronucleus in the M-cell line. Both this report and other experiments with the d48 mutant have shown that transformation with the B gene is not capable of rescuing the A gene after autogamy (24). Gene specificity has also been demonstrated in the d48 mutant by using the G gene from Paramecium primaurelia.…”

Section: Discussionmentioning

confidence: 49%

“…The most intriguing property of the d48 mutant is its ability to be permanently rescued after formation of a new macronucleus if the cloned A gene is transformed into the old macronucleus (5, 10). Recent experiments have shown that the entire A gene is not necessary for d48 rescue; in fact, relatively small portions of the A gene can produce permanent rescue after nuclear reorganization (6,24). Although it has not been possible to localize the rescue activity to one specific region of the A gene, rescue appears to be gene specific, since even the closely related B surface protein gene cannot rescue d48 (24).…”

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confidence: 99%

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Non-Mendelian Inheritance of Macronuclear Mutations Is Gene Specific in Paramecium tetraurelia

Scott

Mikami

Leeck

et al. 1994

Molecular and Cellular Biology

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Paramecium tetraurelia contains two types of nuclei, a diploid germinal micronucleus and a large transcriptionally active macronucleus. The macronuclear genome is formed from the micronuclear DNA during sexual reproduction. Previous studies have shown that the processing of the A-type variable surface protein gene during formation of a new macronucleus is dependent on the presence of the A gene in the old macronucleus. It is not clear if this is a general feature that controls the formation of the Paramecium macronuclear genome or a unique feature of the A locus. Using micronuclear transplantation, we have constructed a strain that has a wild-type micronucleus but has macronuclear deletions of the A- and B-type surface protein genes. Neither the A nor the B gene is incorporated into the new macronucleus after sexual reproduction. Macronuclear transformation of this strain with the B gene rescues the B-gene deletion after formation of the next macronucleus but has not effect on the A deletion. Similarly, transformation with the A gene shows gene-specific rescue for A but not B. The effect of the old macronucleus on the processing of the new macronucleus results in a pattern of non-Mendelian inheritance of both macronuclear deletions. Progeny from the wild-type exconjugant are all wild type, and progeny from the A- B- exconjugant are mutant. The features of this A- B- non-Mendelian mutant demonstrate that the regulation of macronuclear DNA processing is gene specific, and our results open the possibility that this type of regulation affects many regions of the Paramecium genome.

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Section: Resultssupporting

confidence: 68%

Section: Discussionmentioning

confidence: 49%

mentioning

confidence: 99%

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Non-Mendelian Inheritance of Macronuclear Mutations Is Gene Specific in Paramecium tetraurelia

Scott

Mikami

Leeck

et al. 1994

Molecular and Cellular Biology

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Paramecium

Aufderheide¹

2011

Encyclopedia of Life Sciences

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Members of the genus Paramecium (from the classical Greek, παραμηκησ, oblong or oval‐shaped) are ciliated protozoa with an elongated shape (length approximately three to four times the width), a uniform distribution of cilia over the cell surface and a ciliated oral groove leading from the anterior of the cell to a midventral deep oral cavity. The oral apparatus is shaped like a funnel, with 12 rows of oral cilia in a helical array inside. Each cell has two distinct types of nuclei: one large, transcriptionally active, polycopy macronucleus and one or more small, transcriptionally inactive micronuclei. Many features make paramecia favourable organisms for the study of many cellular/developmental/genetic aspects of cells. Their large size allows microscopic observations as well as microinjections and cell surgery. They show Mendelian and non‐Mendelian inheritance, and several different epigenetic inheritance patterns. The genome of Paramecium tetraurelia has been sequenced and is available for analysis. Key Concepts: Species of Paramecium are distributed worldwide in freshwater habitats and are easy to cultivate in the laboratory. Morphological differences are not enough to distinguish some species of Paramecium . The genomes of the macronucleus and micronucleus are not identical, even though both are derived from the same zygote nucleus. The genome of Paramecium tetraurelia has been sequenced; 40 000 genes have been identified. Paramecia do not use two of the three ‘stop codons’ for translation termination. The trichocysts of paramecia are examples of a regulated exocytotic process. The cortex of the cell contains the cilia, basal bodies and associated accessory structures. Paramecia can carry a number of different types of endosymbiotic organisms.

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Paramecium

Aufderheide¹

2021

Encyclopedia of Life Sciences

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Members of the genus Paramecium (from the classical Greek, παραμηκης, meaning oblong or oval shaped) are ciliated protozoa with an elongated shape (length approximately three to four times the width), a uniform distribution of cilia over the cell surface and a ciliated oral groove leading from the anterior of the cell to a midventral deep oral cavity. The oral apparatus is shaped like a funnel, with 12 rows of oral cilia in a helical array inside. Each cell has two distinct types of nuclei: one large, transcriptionally active, polycopy macronucleus and one or more small, transcriptionally inactive micronuclei. Many features make paramecia favourable organisms for the study of diverse cellular/developmental/genetic aspects of cells. Their large size allows microscopic observations as well as microinjections and cell surgery. They show Mendelian and non‐Mendelian inheritance and several different epigenetic inheritance patterns. The genomes of Paramecium tetraurelia and others have been sequenced and are available for analysis. Key Concepts Species of Paramecium are distributed worldwide in freshwater habitats and are easy to cultivate in the laboratory. Morphological differences are not enough to distinguish some species of Paramecium. The genomes of the macronucleus and micronucleus are not identical, even though both are derived from the same zygote nucleus. The genomes of Paramecium tetraurelia have been sequenced; 30 000 genes have been identified. Paramecia do not use two of the three ‘stop codons’ for translation termination. The trichocysts of paramecia are examples of a regulated exocytotic process. The cortex of the cell contains the cilia, basal bodies and associated accessory structures. Paramecia can carry a number of different types of endosymbiotic organisms.

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The role of macronuclear DNA sequences in the permanent rescue of a non-mendelian mutation in Paramecium tetraurelia.

Cited by 42 publications

References 18 publications

Non-Mendelian Inheritance of Macronuclear Mutations Is Gene Specific in Paramecium tetraurelia

Non-Mendelian Inheritance of Macronuclear Mutations Is Gene Specific in Paramecium tetraurelia

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