The role of <em>calgranulin B</em> gene on the biological behavior of squamous cervical cancer in vitro and in vivo

Abstract

ObjectiveThe objective of the study was to explore the role of calgranulin B gene on the biological behavior of squamous cervical cancer.MethodsDifferential transcription in calgranulin B gene between human papillomavirus (HPV)-positive and negative cervical cancer groups was identified, and the relationship between calgranulin B gene and matrix metalloproteinase (MMP) genes were explored using The Cancer Genome Atlas database. Subsequently, the role of calgranulin B on the cell proliferation, apoptosis, invasion and migration was investigated, through overexpression and/or underexpression of calgranulin B in cervical cancer cells. In addition, the effect of calgranulin B on the growth of the cervical cancer was studied via constructing xenograft model in BALB/c nude mice that either overexpressed or underexpressed calgranulin B.ResultsCalgranulin B gene transcription in cervical cancer was highly correlated with the high-risk HPV-16 and HPV-45. In addition, overexpression of calgranulin B increased cell proliferation, invasion and migration, whereas it did not significantly affect cell apoptosis. This effect was also confirmed by calgranulin B knockdown assay. Additionally, we found that the transcription of calgranulin B gene was negatively correlated with MMP15 and MMP24 genes, but positively associated with MMP25 genes in cervical cancer. Furthermore, calgranulin B significantly promoted the growth of cervical cancer in vivo.ConclusionCalgranulin B promotes cell proliferation, migration and invasion of squamous cervical cancer, possibly via regulation of MMPs. Whether there are synergistic actions between calgranulin B and HPV-16/HPV-45 infection on the squamous cervical carcinogenesis or progression need further study.

Integrative Bioinformatics Approaches to Screen Potential Prognostic Immune-Related Genes and Drugs in the Cervical Cancer Microenvironment

“…A PPI network for 401 prognostic DEGs was constructed to reveal the interplay between DEGs, and 228 node genes were confirmed. The top modules that we selected from the PPI network have been reported to influence angiogenesis, proliferation, invasiveness, and therapeutic efficacy in cervical cancer ( Yang et al, 2012 ; Zhang et al, 2015 , 2018 ; Zhao et al, 2015 ; Che et al, 2016 ). The GSEA results showed that 228 node genes were highly associated with myeloid leukocyte activation, adaptive immune response regulation, and receptor signaling pathways.…”

S100A9 regulates cisplatin chemosensitivity of squamous cervical cancer cells and related mechanism

“…However, we also found the expression intensity of S100A9 was statistically higher in post-chemotherapy cervical cancer tissues in comparison with that in pre-chemotherapy. 13 In our further study, 15 four squamous cervical cancer cell lines were selected with diverse human papillomavirus expression, SiHa, C-33A, CaSki, and MS751, and found that the S100A9 mRNA and protein were moderately expressed in SiHa cells. In addition, S100A9 could promote squamous cervical cancer cell proliferation, migration and invasion.…”

Recent Advances on the Molecular Mechanism of Cervical Carcinogenesis Based on Systems Biology Technologies

“…Accumulated evidence has demonstrated the reasons by which HPV infection causes carcinogenesis [13]. Two viral oncoproteins E6 and E7 could play a key role in the HPV-infected cervical cancers.…”