Objective: The purpose of this study was to explore whether human papillomavirus (HPV) DNA is present in surgical smoke generated by loop electrosurgical excision procedures (LEEPs). Furthermore, we investigated the impact of this HPV DNA on surgeons. Methods: A total of 134 outpatients with persistent HPV infections treated with LEEP for cervical intraepithelial neoplasia between 2015 and 2016, along with the corresponding LEEP operators, were included. The flow fluorescence in situ hybridization technique was used to detect HPV DNA in exfoliated cervical cells from the patients, in surgical smoke and in nasal epithelial cells from the surgeons before and after LEEP. Results: The positive rates of HPV DNA in the three types of samples mentioned above were 94.8%, 29.9% and 1.5%, respectively. The distribution of HPV subtypes in surgical smoke was identical to that in the cervical specimens. The positive rate of HPV DNA in surgical smoke was significantly increased for greater distances of the suction device from the surgical site. The nasal epithelial cells of two surgeons were positive for HPV DNA, and the genotypes were consistent with those in the corresponding surgical smoke. After a 3–6-month follow-up, the nasal swabs from these two doctors tested negative for HPV DNA. Conclusions: This study demonstrated the presence of HPV DNA in surgical smoke produced by LEEP and the risk of airborne transmission of HPV DNA during the operation. Fortunately, the HPV DNA in the nasopharynx of the operators was not persistent.

Long-term exposure to fine particulate matter (PM 2.5 ) is associated with kidney dysfunction. However, few studies have investigated acute effects of PM 2.5 elemental constituents on renal function. We evaluated associations between personal PM 2.5 and its elemental constituents and kidney function, assessed by an estimated glomerular filtration rate (eGFR) in Biomarkers of Air Pollutants Exposure in the Chinese aged 60−69 study. Seventy one older individuals were visited monthly between September 2018 and January 2019. Each participant wore a PM 2.5 monitor for 72 h, responded to a questionnaire, and underwent a physical examination with blood sampling. Linear mixed-effect models were used to estimate associations between personal PM 2.5 elemental constituents and eGFR. We found that significant changes in eGFR from −1.69% [95% confidence interval (CI): −3.34%, −0.01%] to −3.27% (95% CI: −5.04%, −1.47%) were associated with interquartile range (IQR) increases in individual PM 2.5 exposures at various lag periods (7−12, 13−24, 0−24, 25−48, and 49−72 h). An IQR increase in 72 h moving averages of copper, manganese, and titanium in personal PM 2.5 corresponded to −2.34% (95% CI: −3.67%, −0.99%) to −4.56% (95% CI: −7.04%, −2.00%) changes in eGFR. Personal PM 2.5 and some of its elemental constituents are inversely associated with eGFR in older individuals.

Threshold limit value (5 mg/m) may unfit for ZnO NPs.

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Cervical cancer is one of the common malignancies in women worldwide. Exploration of pathogenesis and molecular mechanism of cervical cancer is pivotal for development of effective treatment for this disease. Recently, systems biology approaches based on high-throughput technologies have been carried out to investigate the expression of some genes and proteins in genomics, transcriptomics, proteomics, and metabonomics of cervical cancer. Compared with traditional methods，systems biology technology has been shown to provide large of information regarding prognostic biomarkers and therapeutic targets for cervical cancer. These molecular signatures from system biology technology could be useful to understand the molecular mechanisms of cervical cancer development and progression, and help physicians to design targeted therapeutic strategies for patients with cervical cancer.

The levels of E-cadherin are regulated by multiple processes, such as gene transcription, post-translational modification, and protein turnover at the membrane. 11 E-cadherin transcription is directly regulated by methylation of promoter activity. Methylation is a common DNA modification mediated by a family of DNA methyltransferase enzymes that catalyze the addition of a methyl group to cytosine residues at CpG dinucleotides. 12 In most cancers, E-cadherin expression is downregulated due to promoter

Oxidative stress, inflammation and gut microbiota disorders can be induced by long-term high-fat diet (HFD). In order to confirm polyphenols can improve these symptoms. Polyphenols extracted from Shanxi-aged vinegar (SAVEP)...

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