The Role of Long Non-Coding RNA NNT-AS1 in Neoplastic Disease

Zhou, Cong; Duan, Shiwei

doi:10.3390/cancers12113086

Cited by 17 publications

(10 citation statements)

References 70 publications

(112 reference statements)

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“…Among these biomarkers, there is no research reporting the role of FIRRE and NNT-AS1 in UCEC. But NNT-AS1, the regulatory mechanism of microRNA (miRNA) sponge, may affect tumor cell proliferation, invasion, metastasis, and apoptosis 74 and has been found to be involved in the progress of pneumonia. 75 FIRRE can promote the growth of DLBCL cells.…”

Section: Discussionmentioning

confidence: 99%

Pyroptosis-related lncRNAs are potential biomarkers for predicting prognoses and immune responses in patients with UCEC

Liu

Geng

et al. 2022

Molecular Therapy - Nucleic Acids

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Section: Discussionmentioning

confidence: 99%

Pyroptosis-related lncRNAs are potential biomarkers for predicting prognoses and immune responses in patients with UCEC

Liu

Geng

et al. 2022

Molecular Therapy - Nucleic Acids

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“… 8 They have limited protein-coding potential but have been considered a very hot research topic in biology. 9–11 lncRNAs are engaged in a wide array of diverse physiological and pathological processes through gene regulation at the transcriptional, post-transcriptional, or translational level. 12 , 13 Currently, lncRNAs have received considerable attention as novel critical controllers of cancer oncogenesis and progression.…”

Section: Introductionmentioning

confidence: 99%

LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis

Li¹,

Fan²,

Liu³

et al. 2021

CMAR

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Purpose The expression profile, clinical effects, and detailed roles of NOP14 antisense RNA 1 (NOP14-AS1) in tongue squamous cell carcinoma (TSCC) remain ambiguous and need to be further explored. Thus, this work was initiated to offer further solid evidence regarding the expression and roles of NOP14-AS1 in TSCC. Furthermore, additional efforts were exerted to reveal the molecular events by which NOP14-AS1 affects the malignant behaviours of TSCC. Methods NOP14-AS1 expression was detected in TSCC tissues and cell lines using quantitative reverse transcription-polymerase chain reaction. Cell Counting Kit-8 assay, flow cytometric analysis, Transwell migration and invasion assays, and xenograft tumor model analysis were performed to assess the malignant biological behaviors of TSCC cells after NOP14-AS1 depletion. Mechanistic studies were performed using bioinformatics analysis, luciferase reporter assay, RNA immunoprecipitation, and rescue experiments. Results NOP14-AS1 upregulation was identified in TSCC tissues and cell lines. Patients with TSCC exhibiting a high NOP14-AS1 expression faced shorter overall survival than those with a low NOP14-AS1 expression. Functionally, NOP14-AS1 depletion facilitated apoptosis and impeded cell proliferation, migration, and invasion in TSCC. In vivo, the growth of TSCC cells was hindered by NOP14-AS1 depletion. Mechanically, NOP14-AS1 functioned as a competing endogenous RNA by sponging microRNA-665 (miR-665), thereby overexpressing the target high mobility group box 3 (HMGB3) of miR-665. Lastly, rescue experiments confirmed that the introduction of HMGB3 overexpression plasmid or miR-665 inhibitor could abrogate the inhibition of aggressive phenotypes triggered by NOP14-AS1 knockdown. Conclusion NOP14-AS1 executed pro-oncogenic activities in TSCC cells by targeting the miR-665/HMGB3 axis. The NOP14-AS1/miR-665/HMGB pathway may be a valuable prognostic indicator and therapeutic target for preventing TSCC.

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“…Long non-coding RNA (lncRNA), non-coding RNA longer than 200 nucleotides, demonstrated important roles in carcinogenesis 16 , 17 . LncRNAs could modulate various biological functions by regulating mRNA expression through sponging miRNAs 18 , 19 . In this study, we firstly studied the role of NSE in the migration, invasion and EMT process of SCLC.…”

Section: Introductionmentioning

confidence: 99%

NSE, positively regulated by LINC00657-miR-93-5p axis, promotes small cell lung cancer (SCLC) invasion and epithelial-mesenchymal transition (EMT) process

Lü¹,

Zha²,

Zhang³

et al. 2021

Int. J. Med. Sci.

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Background: Neuron specific enolase (NSE) is a specific biomarker for SCLC. However, the biological roles and aberrant expression of NSE in SCLC have not been well illustrated. Methods: The expression of NSE, miR-93-5p and LINC00657 in SCLC tissues and cell lines were detected using real time quantitative PCR (qRT-PCR) or immunohistochemistry. CCK8 assay was performed to detect cell proliferation. Cell migration and invasion capabilities were investigated by transwell assay. Epithelial-mesenchymal transition (EMT) process was verified by detecting epithelial marker E-cadherin and mesenchymal marker N-cadherin. The direct interactions between miR-93-5p and NSE or LINC00657 were predicted by bioinformatics tools and verified using dual luciferase reporter assay. Results: Upregulated expression of NSE in SCLC tumor tissues were positively associated with advanced tumor stage, distant metastasis and poor overall survival. Overexpression of NSE promoted cell proliferation, migration, invasion and EMT in SCLC cells, while silence of NSE inhibited these effects. Mechanically, NSE expression was positively correlated with LINC00657, and negatively correlated with miR-93-5p. Moreover, NSE was positively regulated by LINC00657 through sponging of miR-93-5p. LINC00657 and miR-93-5p promoted SCLC cell migration, invasion and EMT by NSE-mediated manner. Conclusion: Overall, our study revealed a novel role of NSE in SCLC. NSE was positively regulated by LINC00657 through competitively interacting with miR-93-5p, which may be potential targets for SCLC patients.

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The Role of Long Non-Coding RNA NNT-AS1 in Neoplastic Disease

Cited by 17 publications

References 70 publications

Pyroptosis-related lncRNAs are potential biomarkers for predicting prognoses and immune responses in patients with UCEC

Pyroptosis-related lncRNAs are potential biomarkers for predicting prognoses and immune responses in patients with UCEC

LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis

NSE, positively regulated by LINC00657-miR-93-5p axis, promotes small cell lung cancer (SCLC) invasion and epithelial-mesenchymal transition (EMT) process

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