LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis

Li, Jiayi; Fan, Sanhong; Liu, Shuang; Yang, Guang; Jin, Qingsong; Zhang, Xiao

doi:10.2147/cmar.s293322

Cited by 9 publications

(7 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HMGB3, from the high-mobility group box subfamily, participates in the cell proliferation, metastasis, and apoptosis in cancers ( 18 , 22 ). Consistent with results of the previous studies ( 20 , 30 , 31 ), we found that HMGB3 was upregulated in EOC tissues and cells. In vivo experiments further confirmed that HMGB3 knockdown diminished the tumor volume and reduced the protein level of Ki-67 in mice.…”

Section: Discussionsupporting

confidence: 93%

Glaucocalyxin A Inhibits the Malignant Progression of Epithelial Ovarian Cancer by Affecting the MicroRNA-374b-5p/HMGB3/Wnt-β-Catenin Pathway Axis

et al. 2022

View full text Add to dashboard Cite

ObjectiveGlaucocalyxin A (GLA) is an ent-kaurene diterpenoid from Rabdosia japonica var possessing anti-tumor activity. This study aimed to investigate effects of GLA on epithelial ovarian cancer (EOC) and elucidate underlying mechanisms.MethodsThe expression of HMGB3 in EOC tissues was analyzed by GEPIA and immunohistochemistry. Cell proliferation was determined using CCK-8 and colony formation assays. Cell invasion, migration, and apoptosis were detected using Transwell, wound healing, and flow cytometry assays, respectively. Interactions between HMGB3 and miRNAs were predicted using ENCORI and validated using a dual-luciferase assay. mRNA expression levels of HMGB3 and miRNAs were measured using qPCR. Protein expression levels of HMGB3, E-cadherin, N-cadherin, Wnt3a,β-catenin, Bcl-2, and Bax were measured by western blotting. A tumor xenograft model was established to validate the efficacy and mechanism of GLA in vivo.ResultsHMGB3 was upregulated in EOC tissues and cells. GLA dose-dependently inhibited EOC cell proliferation and epithelial-mesenchymal transition (EMT). HMGB3 overexpression promoted proliferation, invasion, migration, and EMT, and suppressed the apoptosis of EOC cells. In addition, miR-374b-5p was targeted by HMGB3, and its overexpression hindered malignant characteristics of EOC cells. HMGB3 overexpression weakened antitumor effects of GLA and miR-374b-5p in EOC cells. Moreover, the Wnt-β-catenin pathway was inhibited by the GLA-mediated miR-374b-5p/HMGB3 axis. In vivo experiments showed that GLA inhibited EOC tumor growth, meanwhile, upregulated the miR-374b-5p level and downregulated the expression of HMGB3, Wnt3a, and β-catenin in tumor tissues.ConclusionsGLA suppressed the malignant progression of EOC by regulating the miR-374b-5p/HMGB3/Wnt-β-catenin pathway axis.

show abstract

Section: Discussionsupporting

confidence: 93%

Glaucocalyxin A Inhibits the Malignant Progression of Epithelial Ovarian Cancer by Affecting the MicroRNA-374b-5p/HMGB3/Wnt-β-Catenin Pathway Axis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Functionally, NOP14‐AS1 depletion promotes apoptosis and impedes cell proliferation, migration, and invasion in TSCC. 33 Zhang et al 34 reported that LINC01564 suppresses the inhibitory impact of miR‐107/103a‐3p on phosphoglycerate dehydrogenase gene expression, increasing HCC development. Furthermore, Ke et al discovered that elevated LINC01564 expression is linked to a worse prognosis in patients with testicular cancer.…”

Section: Discussionmentioning

confidence: 99%

“…High NOP14‐AS1 expression is associated with poor prognosis in TSCC patients. Functionally, NOP14‐AS1 depletion promotes apoptosis and impedes cell proliferation, migration, and invasion in TSCC 33 . Zhang et al 34 reported that LINC01564 suppresses the inhibitory impact of miR‐107/103a‐3p on phosphoglycerate dehydrogenase gene expression, increasing HCC development.…”

Section: Discussionmentioning

confidence: 99%

A hypoxia‐related lncRNA model for prediction of head and neck squamous cell carcinoma prognosis

Xiang

et al. 2022

Cancer Medicine

View full text Add to dashboard Cite

Background Head and neck squamous cell carcinoma (HNSCC) is one of the most common and highly heterogeneous malignancies worldwide. Increasing studies have proven that hypoxia and related long non‐coding RNA (lncRNA) are involved in the occurrence and prognosis of HNSCC. The goal of this work is to construct a risk assessment model using hypoxia‐related lncRNAs (hrlncRNAs) for HNSCC prognosis prediction and personalized treatment. Methods Transcriptome expression matrix, clinical follow‐up data, and somatic mutation data of HNSCC patients were obtained from The Cancer Genome Atlas (TCGA). We used co‐expression analysis to identify hrlncRNAs, then screened for differentially expressed lncRNAs (DEhrlncRNAs), and paired these DEhrlncRNAs. The risk model was established through univariate, least absolute shrinkage and selection operator (LASSO), and stepwise multivariate Cox regression. Finally, we assessed the model from multiple perspectives of tumor mutation burden (TMB), tumor immune infiltration, chemotherapeutic sensitivity, immune checkpoint inhibitor (ICI), and functional enrichment. Results The risk assessment model included 14 hrlncRNA pairs. The risk score was observed to be a reliable prognostic factor. The high‐risk patients had an unfavorable prognosis and significant differences from the low‐risk group in TMB and tumor immune infiltration. In the high‐risk patients, the common immune checkpoints were down‐regulated, including CTLA4 and PDCD1, and the sensibility to paclitaxel and docetaxel was higher. The functional enrichment analysis suggested that the low‐risk group was accompanied by activated immune function. Conclusions The risk assessment model of 14‐hrlncRNA‐pairs demonstrated a promising prognostic prediction for HNSCC patients and can guide personalized clinical treatment.

show abstract

“…A common experimental strategy identifies a differentially expressed antisense transcript in cancer vs. untransformed cells followed by miRNA target gene prediction and experimental validation [14], ideally demonstrating the direct interaction between miRNA and target antisense transcript [47]. Alternatively, immunoprecipitation of Ago2 followed by RT-qPCR was used to corroborate the interaction between the miRNA and its target antisense transcript [48].…”

Section: Discussionmentioning

confidence: 99%

Interdependent Transcription of a Natural Sense/Antisense Transcripts Pair (SLC34A1/PFN3)

Zinad

Sae-Lee

Ariza-Mateos

et al. 2022

ncRNA

View full text Add to dashboard Cite

Natural antisense transcripts (NATs) constitute a significant group of regulatory, long noncoding RNAs. They are prominently expressed in testis but are also detectable in other organs. NATs are transcribed at low levels and co-expressed with related protein coding sense transcripts. Nowadays NATs are generally considered as regulatory, long noncoding RNAs without closer focus on the inevitable interference between sense and antisense expression. This work describes a cellular system where sense and antisense transcription of a specific locus (SLC34A1/PFN3) is induced using epigenetic modifiers and CRISPR-Cas9. The renal cell lines HEK293 and HKC-8 do not express SLC34A1/PFN3 under normal culture conditions. Five-day exposure to dexamethasone significantly stimulates sense transcript (SLC34A1) levels and antisense (PFN3) minimally; the effect is only seen in HEK293 cells. Enhanced expression is paralleled by reduced sense promoter methylation and an increase in activating histone marks. Expression is further modulated by cassettes that stimulate the expression of sense or antisense transcript but disrupt protein coding potential. Constitutive expression of a 5′-truncated SLC34A1 transcript increases sense expression independent of dexamethasone induction but also stimulates antisense expression. Concordant expression is confirmed with the antisense knock-in that also enhances sense expression. The antisense effect acts on transcription in cis since transient transfection with sense or antisense constructs fails to stimulate the expression of the opposite transcript. These results suggest that bi-directional transcription of the SLC34A1/PFN3 locus has a stimulatory influence on the expression of the opposite transcript involving epigenetic changes of the promoters. In perspective of extensive, previous research into bi-directionally transcribed SLC34A loci, the findings underpin a hypothesis where NATs display different biological roles in soma and germ cells. Accordingly, we propose that in somatic cells, NATs act like lncRNAs–with the benefit of close proximity to a potential target gene. In germ cells, however, recent evidence suggests different biological roles for NATs that require RNA complementarity and double-stranded RNA formation.

show abstract

LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis

Cited by 9 publications

References 38 publications

Glaucocalyxin A Inhibits the Malignant Progression of Epithelial Ovarian Cancer by Affecting the MicroRNA-374b-5p/HMGB3/Wnt-β-Catenin Pathway Axis

Glaucocalyxin A Inhibits the Malignant Progression of Epithelial Ovarian Cancer by Affecting the MicroRNA-374b-5p/HMGB3/Wnt-β-Catenin Pathway Axis

A hypoxia‐related lncRNA model for prediction of head and neck squamous cell carcinoma prognosis

Interdependent Transcription of a Natural Sense/Antisense Transcripts Pair (SLC34A1/PFN3)

Contact Info

Product

Resources

About