The Role of Inorganic Polyphosphates in Stress Response and Regulation of Enzyme Activities in Yeast

Kulakovskaya, T. V.; Ryasanova, L. P.; Дмитриев, В. В.; Zvonarev, Anton

doi:10.1007/978-3-319-41073-9_1

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…Starting in the early 1980s, she identified PHB of chain lengths in the range of 100 to 200 HB units as components of ion channels in cell membranes, where it is associated with polyphosphate (PPi), another orphan biopolymer (Table 1). [15][16][17][18][19][20][21][22][23] Furthermore, Reusch found that PHB of chain lengths below ca. 30 units occurs as an appendage to proteins, formed by post-translational PHBylation.…”

Section: Brief Overview Of Phb Historymentioning

confidence: 99%

No Life on this Planet Without PHB

Seebàch

2023

Helvetica Chimica Acta

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The history of discovery by Rosetta Reusch of oligo-and poly-β-hydroxybutyrates (OHBs and PHBs) consisting of less than ca. 150 HB units is described. These 'short-chain' biopolymers can be detected in all living organisms and have numerous physiological activities of fundamental importance for the chemistry of life. The largest are components of ion channels such as Ca 2 + -polyphosphate-PHB (Ca-PPi-PHB) in genetically competent E. coli and in mammalian mitochondria. Sequences with chain lengths < ca. 30 occur covalently attached to proteins (posttranslational PHBylation), and methyl esters of the dimer and trimer are used by certain bacteria as highly efficient antioxidants. With synthetic monodisperse OHBs (up to 128mer) our group has contributed structural investigations, and we have shown that OHBs � 16 alone make phospholipid bilayer vesicles permeable to Ca ions. An extensive biochemical analysis of the TRPM8 protein channel, responsible for the sense of heat in our skin, proved to be fully active only when PHBylated. Reasons for the difficulty of detecting OHBs and PHBs are discussed: the polyester chain is highly flexible, and there is ester cleavage by base, acid, nucleophiles, Lewis acids, and heat -in stark contrast to peptides. PHBs may be called a ubiquitous but fleeting species in the chemistry of life -worth being appreciated and studied much more intensively in the future! A speculation about PHB's possible role in prebiotic compartmentalization is presented, and recent uses of compartmentalization in organic synthesis are briefly mentioned. Portions of the figures used herein were presented in a lecture at the International Symposium on Biopolymers on September 13, 2022, in Sion (Switzerland).

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Section: Brief Overview Of Phb Historymentioning

confidence: 99%

No Life on this Planet Without PHB

Seebàch

2023

Helvetica Chimica Acta

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“…polyP are also found extracellularly, in the blood plasma. Due to its late discovery in animals, the elucidation its function in these organisms, in particular humans, has only started more recently , Rao et al 2009, Kulakovskaya et al 2016.…”

Section: Biological Effectsmentioning

confidence: 99%

Amorphous polyphosphate nanoparticles: application of the morphogenetically active inorganic polymer for personalized tissue regeneration

Schröder¹,

Wang²,

Müller³

2019

J. Phys. D: Appl. Phys.

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Inorganic polyphosphate (polyP) is one of the oldest chemical energy-providing molecules in biological systems. This polymer, containing a much longer sequence of high-energy phosphate units than the universal energy donor adenosine triphosphate, has attracted increasing attention for potential biomedical applications because of its diverse metabolic and regulatory functions and its ability to form biologically active nano/microparticles. The morphogenetic properties (stimulation of cell growth and differentiation via gene induction) of polyP nano/microparticles, prepared in an amorphous form, and their combination with hydrogel-forming polymers enabled the development of 3D-printable and hardenable hybrid materials for personalized tissue regeneration/repair. Depending on the counterion of the polyanionic polyP, both the physical (hardness, stiffness) and the biological properties (induction of cytokines, enzymes or structural proteins) of the 3D-printed scaffolds can be modulated. In this way, individualized scaffolds/implants, even loaded with cells, which can be adapted to specific tissue defects (e.g. of bone and cartilage), can be fabricated. This topical review summarizes the physical–chemical properties and biological effects of polyP, formulated as smart amorphous nano/microparticles, and its application to produce personalized 3D-scaffolds/implants that open new possibilities in regenerative medicine.

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“…They play a crucial role in cell growth, regulation of metabolic processes, and cellular response to stress. Since their discovery in yeasts in the 19th century, polyPs have been found in all life kingdoms [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Short‐chain polyphosphates: Extraction effects on migration and size estimation of Saccharomyces cerevisiae extracts with polyacrylamide gel electrophoresis

et al. 2023

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Polyacrylamide gel electrophoresis is commonly used to characterize the chain length of polyphosphates (polyP), more generally called condensed phosphates. After separation, nonradioactive, optical polyP staining is limited to chain lengths greater than 15 monomers with toluidine blue or 4′,6‐diamidino‐2‐phenylindole. PolyP chain lengths longer than 62monomers were correlated to the shortest DNA ladders. In this study, synthetic linear polyPs (Sigma‐Aldrich “Type 45”, estimated mean length of 45 monomers), trimetaphosphate (trimetaP: 3 ring), tripolyphosphate (tripolyP), pyrophosphate (PPi), and inorganic orthophosphate (o‐Pi) were visualized after separation by an in situ hydrolytic degradation process to o‐Pi that was subsequently stained with methyl green. Statistically insignificant migration reduction of synthetic short‐chain polyP after perchloric acid or phenol–chloroform extraction was confirmed with the Friedman test. 31P diffusion–ordered NMR spectroscopy confirmed that extraction also reduced PPi diffusivity by <10%. Linear regression between the Rf peak migration value and the logarithm of synthetic polyP molecular weights enabled estimation of extracted polyP chain lengths from 2 to 45 monomers. Linear polyP extracts from Saccharomyces cerevisiae grown in aerobic conditions were generally shorter than extracts cultured in anaerobic conditions. Extractions from both aerobic and anaerobic S. cerevisiae included tripolyP and o‐Pi, but no PPi.

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The Role of Inorganic Polyphosphates in Stress Response and Regulation of Enzyme Activities in Yeast

Cited by 6 publications

References 36 publications

No Life on this Planet Without PHB

No Life on this Planet Without PHB

Amorphous polyphosphate nanoparticles: application of the morphogenetically active inorganic polymer for personalized tissue regeneration

Short‐chain polyphosphates: Extraction effects on migration and size estimation of Saccharomyces cerevisiae extracts with polyacrylamide gel electrophoresis

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