Short‐chain polyphosphates: Extraction effects on migration and size estimation of Saccharomyces cerevisiae extracts with polyacrylamide gel electrophoresis
Abstract:Polyacrylamide gel electrophoresis is commonly used to characterize the chain length of polyphosphates (polyP), more generally called condensed phosphates. After separation, nonradioactive, optical polyP staining is limited to chain lengths greater than 15 monomers with toluidine blue or 4′,6‐diamidino‐2‐phenylindole. PolyP chain lengths longer than 62monomers were correlated to the shortest DNA ladders. In this study, synthetic linear polyPs (Sigma‐Aldrich “Type 45”, estimated mean length of 45 monomers), tr… Show more
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