The role ofbudABC on 1,3-propanediol and 2,3-butanediol production from glycerol inKlebsiella pneumoniaeCICIM B0057

Abstract

ABSTRACT1,3-propanediol (1,3-PD) is an important compound from which many others can be synthesized. 2,3-butanediol (BDO) is the key by-product in the biosynthesis of 1,3-PD from glycerol, but it impedes its downstream purification. In Klebsiella, the budA, budB and budC genes encode enzymes that are responsible for the synthesis of BDO. In this study, 3 individual antisense RNAs were designed to repress the expression and hence activity of BudA-C. Compared with the parent strains, the activities of BudB and BudC were reduced by 60.5% and 70.5%, respectively, and the mRNA level of budA was reduced by 70%. Decreased BudC activity had no effect on cell growth or carbon distribution. However, reduced BudA and BudB activity decreased the BDO concentration by 35% and led to a 10% increase in the yield of 1,3-PD. This result suggests the activities of BudA and BudB could be key factors in the production of BDO from glycerol in Klebsiella. This study provides a deeper understanding of the role of budABC in glycerol metabolism in Klebsiella.

Poly(alkylene terephthalate)s: From current developments in synthetic strategies towards applications

“…[61][62][63] The 1,3-PDO synthesis from glucose can proceed via two steps with natural organisms or via one step, if the organisms are genetically engineered for that purpose. [64,65] It has been shown that monomers synthesised via this method contain less impurities than the 1,3-PDO produced from petroleum-based reagents. [57] Figure 6.…”

Genome Sequencing of Pantoea agglomerans C1 Provides Insights into Molecular and Genetic Mechanisms of Plant Growth-Promotion and Tolerance to Heavy Metals

“…As regards the indirect means of plant growth promotion, in P. agglomerans C1 genome, we found (Table 4) genes encoding enzymes involved in the synthesis of volatile organic compounds (acetoin and 2,3-butanediol; [43,44], Gamma-Aminobutyric Acid (GABA) [45], and siderophores [46], as well as genes encoding the three components of EfeUOB transporter, a ferrous iron transporter induced by low pH and low iron [47].…”

Reprogramming the metabolism of Klebsiella pneumoniae for efficient 1,3-propanediol production

“…Similar negative results were obtained when disrupting the acetate synthesis pathway by deactivating the ackA-pta gene (data not shown). This might due to the fact that both metabolic pathways play an important role in cell growth by balancing the intracellular acetyl-CoA pool, energy supply, and synthesis of branched-chain amino acids (Lu et al, 2016;Ohtake et al, 2017). Therefore, the 2,3-butanediol and acetate synthesis pathways were remained complete and the strain Kpr-2 was constructed by further deleting the fumarate reductase gene (frdA, GenBank accession no.…”