The role of human papillomavirus in oral squamous cell carcinoma: myth and reality

Kansy, Katinka; Thiele, Oliver C.; Freier, Kolja

doi:10.1007/s10006-012-0383-0

Cited by 51 publications

(42 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, HPV has been discussed as a relevant predictive biomarker for OSCC [10, 11]. Higher rates of HPV+ tumours, better outcomes for HPV+ patients, and improved survival rates compared to HPV− groups have often been published in studies that did not differentiate between OSCC and OPSCC [12, 13] and did not examine other confounders significantly influencing DSS.…”

Section: Introductionmentioning

confidence: 99%

Impact of HPV infection on oral squamous cell carcinoma

et al. 2016

View full text Add to dashboard Cite

BackgroundHead and neck squamous cell carcinomas (HNSCC) are often divided by their aetiology. Noxae associated collectives are compared with the human papilloma virus (HPV)-associated group, whereas different localisations of oral (OSCC) and oropharyngeal (OPSCC) squamous cell carcinomas are mostly discussed as one single group. Our aim was to show that classification by aetiology is not appropriate for OSCC.ResultsHPV DNA was detected by PCR in 7 (3.47%) patients, and we identified 12 (5.94%) positive (+) cases by p16INK4a immunostaining. Only 4 (1.98%) of the p16INK4a+ cases were + for HPV using PCR. Our homogenous collective of OSCC allowed us to compare HPV+ and HPV negative (−) patients without creating bias for tumour localisation, age, gender or tumour stage.Materials and methodsAfter testing OSCC samples for HPV positivity, we compared the results of two commonly used HPV detection methods, p16INK4a immunostaining and HPV DNA-related PCR, on 202 OSCC patients. HPV subtypes were determined with an HPV LCD Array Kit. Clinicopathological features of the patients were analysed, and the disease specific survival rates (DSS) for HPV+ and HPV− patients were obtained.Conclusionsp16INK4a immunostaining is a not a reliable HPV detection method for OSCC. Positive p16INK4a immunostaining did not agree with + results from PCR of HPV DNA. Furthermore, the influence of HPV-related oncogenic transformation in OSCC is overestimated. The significance of HPV infection remains clinically unclear, and its influence on survival rates is not relevant to OSCC cases.

show abstract

Section: Introductionmentioning

confidence: 99%

Impact of HPV infection on oral squamous cell carcinoma

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Its main risk factors are smoking and drinking habits [2], and evidences point the human papillomavirus (HPV) infection as a potential risk for OSCC [3, 4]. Besides surgery, radiotherapy and chemotherapy are often employed in treatment of oral cancer, and as a consequence of the interaction of radiation and drug therapy, a cancer patient may suffer oral mucositis (incidence of oral mucositis varies significantly depending on the drugs and treatment regimens used) [5, 6].…”

Section: Introductionmentioning

confidence: 99%

Low‐level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway

Sperandio

Giudice

Corrêa

et al. 2013

Journal of Biophotonics

View full text Add to dashboard Cite

Low-level laser therapy (LLLT) is a non-thermal phototherapy used in several medical applications, including wound healing, reduction of pain and amelioration of oral mucositis. Nevertheless, the effects of LLLT upon cancer or dysplastic cells have been so far poorly studied. Head and neck cancer patients receiving LLLT for oral mucositis, for example, might have remaining tumor cells that could be stimulated by LLLT. This study demonstrated that LLLT (GaAlAs – 660 nm or 780 nm, 40 mW, 2.05, 3.07 or 6.15 J/cm2) can modify oral dysplastic cells (DOK) and oral cancer cells (SCC9 and SCC25) growth by modulating the Akt/mTOR/CyclinD1 signaling pathway; LLLT significantly modified the expression of proteins related to progression and invasion in all the cell lines, and could aggravate oral cancer cellular behavior, increasing the expression of pAkt, pS6 and Cyclin D1 proteins and producing an aggressive Hsp90 isoform. Apoptosis was detected for SCC25 and was related to pAkt levels. Translocation of pAkt seen through Immunofluorescence from cytoplasm of control SCC9 cells (A) to nuclei of red (D) and infrared (G) laser irradiated cells. B, E and H: pAkt staining in control, red and infrared laser groups, respectively. C, F and I: nuclear DAPI in control, red and infrared groups, respectively.

show abstract

“…PCR is considered to be highly sensitive and is the most established method for HPV detection in oral rinse sample (13,14). Thus, Taqman real-time PCR assays were used for detection on the ABI Prism 7900 Sequence Detection System with 40 cycles in a reaction (denaturation at 95°C, annealing and extension at 60°C).…”

Section: Hpv and Analytic Methodsmentioning

confidence: 99%

HPV16 infection in oral cavity and oropharyngeal cancer patients

Dang

Feng

2016

J Oral Sci

View full text Add to dashboard Cite

Our objective was to compare archived tissue biopsy samples from the oral cavity and oropharynx, in terms of human papillomavirus (HPV) 16 infection. We used Taqman real-time PCR assay to detect HPV16 in 121 archived biopsy samples from the oral cavity and 100 samples from the oropharynx. Among patients with oral cavity cancer (OCC), 9% (6/65) had HPV16 infection which was significantly less than those with oropharyngeal cancer (OPC) where 79% (39/50) were HPV16 positive (P < 0.001). Our results demonstrated a significant difference between genders where males had a seven times higher chance of having HPV16 infection (P < 0.001). Viral load variation between each group was demonstrated. The median viral load in OPC was similar in OCC cases, but cancer samples were significantly higher than in non-cancer cases (oral cavity samples P = 0.015; oropharynx samples P = 0.09). From our results, we conclude that there is a significant difference in HPV16 detection between OCC and OPC, and HPV16 differs greatly between male and female cancer patients. (J Oral Sci 58, [265][266][267][268][269] 2016)

show abstract

The role of human papillomavirus in oral squamous cell carcinoma: myth and reality

Cited by 51 publications

References 55 publications

Impact of HPV infection on oral squamous cell carcinoma

Impact of HPV infection on oral squamous cell carcinoma

Low‐level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway

HPV16 infection in oral cavity and oropharyngeal cancer patients

Contact Info

Product

Resources

About