The role of HINT1 protein in morphine addiction: An animal model‐based study

Liu, Peng; Chu, Zheng; Liu, Gang; Deng, Lisha; Yang, Lei; Dang, Yonghui

doi:10.1111/adb.12897

Cited by 11 publications

(6 citation statements)

References 44 publications

(56 reference statements)

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“…Liu et al [ 42 ] conducted a study in which they extensively investigated HINT1 protein involvement in key brain areas associated with addiction, including prefrontal cortex, nucleus accumbens, striatum, and hippocampus at different stages of different models. They also investigated the effect of HINT1 protein deletion on morphine addiction using HINT1 knockout mice to establish the above models and a physical dependence model.…”

Section: Discussionmentioning

confidence: 99%

Association of the rs3864283 Polymorphism Located in the HINT1 Gene with Cigarette Use and Personality Traits

Suchanecka

Boroń

Chmielowiec

et al. 2023

IJMS

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Nicotine is the major reinforcing component of tobacco and it is believed that the pharmacological effects of nicotine motivate the initiation and maintenance of a smoking habit. HINT1 appears to play a role in the modulation of the effects of drug abuse. Hence, the aim of this study was the analysis of the association between the rs3864283 polymorphism of the HINT1 gene and cigarette use; the analysis of personality traits assessed by the means of the NEO-FFI Inventory; the analysis of anxiety measured by the STAI questionnaire; and the analysis of the interactions between the rs3864283 and both personality traits and anxiety. The study group consisted of 522 volunteers. Of these, 371 were cigarette users and 151 were never-smokers. The genomic DNA was isolated from venous blood using standard procedures. The results of both inventories, i.e., NEO-FFI and STAI., were reported as the sten scores. Genotyping was conducted with the real-time PCR method. Statistically significant differences were found in the frequency of rs3864283 genotypes and alleles in the tested sample of Cigarette Users when compared to the control group. The Cigarette Users compared to the control group obtained higher scores in the assessment of NEO-FFI extraversion scale, and significantly lower results were obtained for the NEO-FFI openness scale, the agreeableness scale, and the conscientiousness scale. There was a statistically significant effect of rs3864283 genotype interaction and Cigarette Use or not using (control group) on the extraversion scale. There was also a statistically significant effect of Cigarette Users or the control group on the extraversion scale score. The results obtained in the presented study indicated a significant association between the HINT1 rs3864283 variant and smoking status. Moreover, this is the first study incorporating genetic association of above-mentioned polymorphic site with interaction analysis of personality traits and anxiety. Overall, the results of this study suggest that HINT1 is an important genetic component associated with nicotine usage mechanisms.

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Section: Discussionmentioning

confidence: 99%

Association of the rs3864283 Polymorphism Located in the HINT1 Gene with Cigarette Use and Personality Traits

Suchanecka

Boroń

Chmielowiec

et al. 2023

IJMS

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“…Taken together, we hypothesize that RGS protein degradation, possibly mediated by UPS, might be related with opioid addiction. In this study, we aimed to conduct a preliminary investigation of the regulatory roles of RGS4 and UPS in the development of morphine‐induced behavioral sensitization in rats, a well‐recognized animal model of opioid addiction (Liu et al., 2021; Trombin et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

The roles of ubiquitin–proteasome system and regulator of G protein signaling 4 in behavioral sensitization induced by a single morphine exposure

et al. 2023

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Aims Opioid addiction is a major public health issue, yet its underlying mechanism is still unknown. The aim of this study was to explore the roles of ubiquitin–proteasome system (UPS) and regulator of G protein signaling 4 (RGS4) in morphine‐induced behavioral sensitization, a well‐recognized animal model of opioid addiction. Methods We explored the characteristics of RGS4 protein expression and polyubiquitination in the development of behavioral sensitization induced by a single morphine exposure in rats, and the effect of a selective proteasome inhibitor, lactacystin (LAC), on behavioral sensitization. Results Polyubiquitination expression was increased in time‐dependent and dose‐related fashions during the development of behavioral sensitization, while RGS4 protein expression was not significantly changed during this phase. Stereotaxic administration of LAC into nucleus accumbens (NAc) core inhibited the establishment of behavioral sensitization. Conclusion UPS in NAc core is positively involved in behavioral sensitization induced by a single morphine exposure in rats. Polyubiquitination was observed during the development phase of behavioral sensitization, while RGS4 protein expression was not significantly changed, indicating that other members of RGS family might be substrate proteins in UPS‐mediated behavioral sensitization.

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“…The hippocampus is a key brain area for learning and memory, and it has direct circuitry and functional connections with the ventral tegmental area, the nucleus accumbens, and other addictionrelated brain areas [2,3] . Therefore, the hippocampus is also involved in opioid addiction at the genetic, molecular, cellular, synaptic plasticity, and neural circuit levels [1,2,[4][5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Extinction for Morphine-CPP on Hippocampal EEG Power Spectrum in Mice

Gao

WANG

et al. 2022

Wuhan Univ. J. Nat. Sci.

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The relationship between hippocampal electroencephalogram (EEG) power spectrum and the extinction of addiction memory was investigated. Forty KM mice (Kunming mice) that had successfully established morphine (MOR)-conditioned place preference (CPP) were divided into four groups: saline-training extinction (SAL-TE), SAL-natural extinction (SAL-NE), MOR-TE, MOR-NE, for extinction treatment and EEG recording in the dorsal and ventral hippocampus (DH/VH). Results show that the CPP score of MOR-TE significantly decreased and the total, β and γ bands power spectrum of MOR-TE was suppressed. Notably, the total power from the VH was more correlated with the extinction method, and the TE total power was always lower than NE in SAL or MOR group. Naloxone enhanced the EEG total power and all bands (DH) or β and γ bands (VH) power in the SAL group. However, in the MOR-TE group, only β and γ bands power in VH increased (P<0.05). The results suggest that TE can promote the extinction of opiate addictive memory more effectively than NE, and the extinction of MOR-CPP may have a stronger correlation with changes of VH-EEG power spectrum.

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The role of HINT1 protein in morphine addiction: An animal model‐based study

Cited by 11 publications

References 44 publications

Association of the rs3864283 Polymorphism Located in the HINT1 Gene with Cigarette Use and Personality Traits

Association of the rs3864283 Polymorphism Located in the HINT1 Gene with Cigarette Use and Personality Traits

The roles of ubiquitin–proteasome system and regulator of G protein signaling 4 in behavioral sensitization induced by a single morphine exposure

Effects of Different Extinction for Morphine-CPP on Hippocampal EEG Power Spectrum in Mice

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