The Role of Helper T Cell Products in Mouse B Cell Differentiation and Isotype Regulation

Coffman, Robert L.; Seymour, Brian W. P.; Lebman, Deborah A.; Hiraki, Debra D.; Christiansen, Judy; Shrader, B; Cherwinski, Holly; Savelkoul, H.F.J.; Finkelman, Fred D.; Bond, Michael D.; Mosmann, Tim R.

doi:10.1111/j.1600-065x.1988.tb00739.x

Cited by 730 publications

(378 citation statements)

References 53 publications

Supporting

Mentioning

350

Contrasting

Unclassified

Order By: Relevance

“…However, our results indicate that the T function capable of inducing antitopo I Ab production was associated with individual cytokine profiles rather than Th1/Th2 phenotypes. Th1-like topo I-specific clones could drive B cells to produce anti-topo I Ab if the cultures were supplemented with IL-6, and this result is consistent with the findings observed in mice (41). In addition, most topo I-specific T cell clones expressed both Th1 and Th2 cytokines, and the patterns of cytokine expression were heterogeneous, supporting the concepts that the human T cell cytokine profile is not controlled by a simple binary switch between two sets of genes, and each cytokine gene expression is regulated independently (42).…”

Section: Figuresupporting

confidence: 81%

Analysis of Soluble and Cell Surface Factors Regulating Anti-DNA Topoisomerase I Autoantibody Production Demonstrates Synergy Between Th1 and Th2 Autoreactive T Cells

Kuwana

Medsger

Wright

2000

The Journal of Immunology

View full text Add to dashboard Cite

The cellular and subcellular events governing Ab production with specificity for self Ags are poorly understood. In this study we examined the role of cellular interactions and cytokines in regulating the production of anti-DNA topoisomerase I (topo I) Ab, a major autoantibody in patients with systemic sclerosis (SSc). Topo I-specific T cell clones derived from SSc subjects and healthy donors were cultured with autologous peripheral blood B cells. Anti-topo I Ab production was induced by five of seven topo I-specific T cell clones derived from SSc subjects, but by none of eight T cell clones generated from healthy controls. However, two of the T cell clones from healthy controls provided help to HLA-DR-matched SSc B cells to produce anti-topo I Ab. The analysis of cytokine mRNA expression revealed that the ability to promote anti-topo I autoantibody production was strictly correlated with IL-2 and IL-6 expression by the T cell clones. Kinetic studies showed that IL-2 was required throughout the culture period for maximal autoantibody production and that both MHC-TCR and CD40-CD40L interactions were essential during the early phase of the culture. IL-6 was important in the late phase. Th1 clones (producing IL-2, but no IL-6) and Th2 clones (producing IL-6, but no IL-2) synergically activated autologous B cells to produce anti-topo I Ab. These results indicate that T cell-dependent B cell activation resulting in anti-topo I autoantibody production requires a series of temporally defined cell contact and soluble stimuli.

show abstract

Section: Figuresupporting

confidence: 81%

Analysis of Soluble and Cell Surface Factors Regulating Anti-DNA Topoisomerase I Autoantibody Production Demonstrates Synergy Between Th1 and Th2 Autoreactive T Cells

Kuwana

Medsger

Wright

2000

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Interestingly, there was a dramatic difference in the IgG subclass antibody response elicited by these strains. The IgG2a to IgG1 ratio generated by ILV1 was 4.7, which indicates a Th1-like Ig subclass antibody response [36]. On the other hand, DD3008 generated a IgG2a to IgG1 ratio of 0.3, which represents a Th2-like Ig subclass antibody response.…”

Section: Discussionmentioning

confidence: 98%

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Ulrich

Amemiya

Waag

et al. 2005

Vaccine

View full text Add to dashboard Cite

“…Mucosal or secretory immunity involves induction of precursor IgA-secreting cells in bronchial-associated and gut-associated lymphatic tissue, including Peyer's patches; migration of these cells to mucosal sites (including gut, respiratory tract, mammary gland, lacrimal gland and genitourinary system); and terminal differentiation to IgA-secreting cells in these tissues 42 . TGF-β1 specifically enhances IgA expression in stimulated Peyer's patch and splenic B cells 43,44 and seems to be involved in lymphocyte homing to mucosal sites, enhancing expression of human HML-l and murine β 7 α M290 integrins which are believed to mediate homing to the mucosa 45,46 . On the other hand, TGF-β1 inhibits adhesiveness of Peyer's patch high endothelial venules for lymphocytes 47 .…”

Section: Discussionmentioning

confidence: 99%

Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease

Shull

Ormsby

Kier

et al. 1992

Nature

2,814

1,774

View full text Add to dashboard Cite

show abstract

The Role of Helper T Cell Products in Mouse B Cell Differentiation and Isotype Regulation

Cited by 730 publications

References 53 publications

Analysis of Soluble and Cell Surface Factors Regulating Anti-DNA Topoisomerase I Autoantibody Production Demonstrates Synergy Between Th1 and Th2 Autoreactive T Cells

Analysis of Soluble and Cell Surface Factors Regulating Anti-DNA Topoisomerase I Autoantibody Production Demonstrates Synergy Between Th1 and Th2 Autoreactive T Cells

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease

Contact Info

Product

Resources

About