The role of filtration in the provision of leukocyte poor red cells to multitransfused patients

James, J.; Matthews, R. N.; Holdsworth, Rhonda; Fulton, Alison; Tauro, G. P.; Hussein, Sonay; McGrath, K.M.

doi:10.3109/00313028609090839

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…Thalassemic patients represented an ideal setting to verify the usefulness of ABT leukodepletion. Although leukodepletion reduces non-hemolytic febrile reactions (NHFR)88–90 and anti-leukocyte antibodies and anti-platelet production91, 92 it does not modify substantially the immunologic alterations observed in thalassemic patients 92 Probably, their pathogenesis is very complex and TRIM represents only one of the numerous factors interfering with immunity.…”

Section: Etiology Of Risks Of Infections In Thalassemia and Hemoglobimentioning

confidence: 99%

Infections in Thalassemia and Hemoglobinopathies

Ricerca¹,

Girolamo²,

Rund³

2009

Medit J Hemat Infect Dis

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The clinical approach to thalassemia and hemoglobinopathies, specifically Sickle Cell Disease (SCD), based on transfusions, iron chelation and bone marrow transplantation has ameliorated their prognosis. Nevertheless, infections still may cause serious complications in these patients. The susceptibility to infections in thalassemia and SCD arises both from a large spectrum of immunological abnormalities and from exposure to specific infectious agents. Four fundamental issues will be focused upon as central causes of immune dysfunction: the diseases themselves; iron overload, transfusion therapy and the role of the spleen. Thalassemia and SCD differ in their pathogenesis and clinical course. It will be outlined how these differences affect immune dysfunction, the risk of infections and the types of most frequent infections in each disease. Moreover, since transfusions are a fundamental tool for treating these patients, their safety is paramount in reducing the risks of infections. In recent years, careful surveillance worldwide and improvements in laboratory tests reduced greatly transfusion transmitted infections, but the problem is not completely resolved. Finally, selected topics will be discussed regarding Parvovirus B19 and transfusion transmitted infections as well as the prevention of infectious risk postsplenectomy or in presence of functional asplenia.

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Section: Etiology Of Risks Of Infections In Thalassemia and Hemoglobimentioning

confidence: 99%

Infections in Thalassemia and Hemoglobinopathies

Ricerca¹,

Girolamo²,

Rund³

2009

Medit J Hemat Infect Dis

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“…By removal of the buffy coat approximately 70% to 90% of the leukocytes can be depleted, with red blood cell loss ranging from 10% to 40%. 23, 24,44,45 However, complete separation of the various cellular components cannot be obtained by centrifugation. The depletion of leukocytes can be improved by a second centrifugation step after the addition of an isotonic saline solution to the packed red blood cells, but this procedure causes a substantial loss of red cells.…”

Section: Techniques To Prepare Leukocyte-poor Bloodmentioning

confidence: 99%

“…Depending on the process conditions, automatic cell washers can remove 70% to 95% of the leukocytes from whole blood, whereas the loss of red blood cells is approximately 15%. 23, 24,48,49 The major advantage of this method is its efficiency in removing more than 95% of the plasma from whole blood. 48'49 Plasma removal may be beneficial in reducing the risk of virus transmission.…”

Section: Techniques To Prepare Leukocyte-poor Bloodmentioning

confidence: 99%

The mechanisms of leukocyte removal by filtration

Bruil

Beugeling

Feijén

et al. 1995

Transfusion Medicine Reviews

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“…The patient population served by our unit has been previously described [12]. NHFTR's were defined as a rise in temperature above 38 °C during or within 4 h of transfusion.…”

Section: Transfusion Practicementioning

confidence: 99%

“…Our policy of staging the treatment of these patients, so that products which are progressively more leucocyte-poor are given as patients experience reactions to their current product, has been previously described [12]. Under this policy, patients reacting to filtered cells were treated with red cells prepared by filtration and washing or freeze-thawing.…”

mentioning

confidence: 99%

Relative Efficiency of Leucocyte Removal Procedures for the Production of Leucocyte-Poor Red Cell Concentrates Assessed by Flow Cytometry

Farrugia¹,

Tan²,

Romeo³

et al. 1994

Vox Sanguinis

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Flow cytometry was used to: (1) determine residual leucocyte numbers in red cell suspensions following the range of leucocyte depletion procedures used in our organisation, and (2) to characterise phenotypically the leucocytes using direct immuofluorescence with monoclonal antibodies to cell surface receptors. Under the conditions used, a lower limit of detection of 2.5 leucocytes per µl (equivalent to 3.43 log(10) or 99.96% removal) could be achieved. Filtration through polyester filters was found to remove up to >99.96% of the initial leucocytes; however, a significant differential efficacy was observed between filters from different manufacturers even when filters with similar costs were compared. The order of filter brands with respect to leucocyte removal found was Pall BPF4 = Erypur Optima G-O > Sepacell R500 > Pall RC50. Pheno- typing revealed that increasing filtration efficacy was associated with a preferential removal of lymphocytes; conversely, a second filtration over one brand of filter allowed proportionately more lymphocytes to pass through compared with the first filtration. A saline wash following filtration removed a further 0.5% of the initial leucocyte content, and was associated with a preferential loss of granulocytes. Freeze-thawing the red cell suspension removed fewer leucocytes (96.3%) than did filtration (98.74% to >99.6%) or filtration followed by washing (99.22%), and also led to preferential loss of granulocytes. Flow cytometry provides a reliable tool for the quality control of leuco-depleted red cells, and allows a qualitative assessment of the residual leucocytes. This information is of value in choosing procedures aimed at decreasing the risk of alloimmunisation and post-transfusion reactions.

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The role of filtration in the provision of leukocyte poor red cells to multitransfused patients

Cited by 7 publications

References 13 publications

Infections in Thalassemia and Hemoglobinopathies

Infections in Thalassemia and Hemoglobinopathies

The mechanisms of leukocyte removal by filtration

Relative Efficiency of Leucocyte Removal Procedures for the Production of Leucocyte-Poor Red Cell Concentrates Assessed by Flow Cytometry

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