The role of derivatization techniques in the analysis of glyphosate and aminomethyl-phosphonic acid by chromatography

Effect of glyphosate on X-ray diffraction of copper films prepared by electrochemical deposition

“…3,4 Therefore, a sensitive detection of glyphosate is highly demanded. Until now, the reliable detection methods for glyphosate are the chromatographic methods including gas chromatography tandem mass spectrometry (GC-MS), 5,6 liquid chromatography tandem mass spectrometry (LC-MS) [7][8][9] and chemical sensing methods. [10][11][12] Despite high sensitivity and promising stability, the chromatographic methods require complicated sample pre-treatment and chemical derivatization processes to facilitate the detection or the separation of the analytes.…”

Quantification of Glyphosate and AMPA by HPLC-ICP-MS/MS and HPLC-DAD: A Comparative Study

“…The O 2 was introduced as reaction gas in the CRC, and the second quadrupole was adjusted to monitor the reaction product m/z 47 ( 31 P 16 O + ), so that these ions were detected with minimal interference: 20 31 P + + 16 O 2 → 31 P 16 O + + 16 O ΔH reaction = −3.17 eV 1The analytical conditions were: ZORBAX SB-C8 (4.6 × 150 mm, 5 μm), injection volume 50 μL, mobile phase composed of 50 mM acetic acid/ammonium acetate solution: 5 mM tetrabutylammonium: 1% methanol with pH = 4.7, isocratic elution with 1.0 mL min −1 . 23 The HPLC-ICP-MS/MS analysis conditions are summarized in Table 1.…”

“…Derivatization is not a simple process, even though it confers higher sensitivity and selectivity to the studied compounds. 16 Several factors could affect the efficiency of the reaction, hindering the analysis of complex matrices because interferents present in these matrices could decrease the reaction performance and the compound quantification. In addition, the applicability of derivatization agents should be evaluated for each matrix, because the reaction developed for one matrix may not work in another with different characteristics, which contributes to the increased cost, sample preparation and analysis time.…”

“…In addition, all these factors may contribute to an increase in standard deviation and process cost. 16 For toxicological and environmental monitoring, several countries and organizations have established significantly different maximum residue limits (MRLs) for glyphosate in drinking water such as 700 μg L -1 by the US EPA, 17 and less than 0.1 μg L -1 by European legislation 18 for this matrix. Therefore, several analytical methods have been developed to determine glyphosate at concentrations below these limits.…”

Studying method of underivatized determination glyphosate, glufosinate and their metabolites in drinking water

“…According to international research studies, determining glyphosate, glufosinate and their metabolites concentration has been investigated in different kinds of samples, whereas direct determination of these compounds in environmental samples has a number of difficulties such as simple structure of the compounds, few functional groups, high polarity and easily complexing with components presenting in the samples. [4][5][6] Chromatography is an useful method to determine the residual quantities of glyphosate, glufosinate and their metabolites in food and the environment. Moreover, an indirect analysis method with the help of the derivation technique has overcome problems associated with the compound structures, and 9-fluorenylmethylchloroformate (FMOC-Cl) is a derivative agent intensively utilized in liquid chromatography because this substance brings about stability of retention time when analyzing compounds.…”