The role of cyanide‐resistant respiration in Solanum tuberosum L. against high light stress

Abstract

Cyanide-resistant respiration in potato mitochondria is an important pathway for energy dissipation. It can be activated by high light; however, it is unclear what roles cyanide-resistant respiration plays in the response to high light stress in potato.• We designed a CRISPR vector for the functional gene StAOX of the potato cyanide-resistant respiratory pathway. Agrobacterium tumefaciens GV3101 was transformed into potato. Hydrogen peroxide level, MDA content, antioxidant activity and cyanide-resistant respiratory capacity of potato leaves under high light stress were determined. Photosynthetic efficiency and chlorophyll content were determined. In addition, the operation of the malate-oxaloacetate shuttle route and transcription level of photorespiration-related enzymes were also examined.• The results showed that two base substitutions occurred at the sequencing target site on leaves of the transformed potato. Accumulation of ROS and increased membrane lipid peroxidation were detected in the transformed potato leaves and lower photosynthetic efficiency was observed. The transcription level of the malate-oxaloacetate shuttle route and photorespiration-related enzymes also significantly increased.• These results indicate that the cyanide-resistant respiration is an important physiological pathway in potato in response to high light stress. It also suggests that plant cyanide-resistant respiration is closely related to photosynthesis. This implies the unexplored importance of plant cyanide-resistant respiration in plant photosynthesis, energy conversion and carbon skeleton formation.Plant Biology 22 (2020) 425-432

24-Epibrassinolide Reduces Drought-Induced Oxidative Stress by Modulating the Antioxidant System and Respiration in Wheat Seedlings

“…It should be noted that stressed wheat plants were distinguished by a two-fold increase in the capacity of the alternative respiratory pathway relative to the control ( Figure 5 F), which, apparently, is important for plants under oxidative stress due to the detection of antioxidant activity in cyanide-resistant oxidase [ 81 ]. Moreover, alternative oxidase has been considered to have a role in improving plant tolerance to multiple environmental stresses [ 52 , 53 ]. Indeed, it was shown that the alternative respiration rate was significantly increased in stressful conditions, for instance, under salt [ 82 ], cold [ 83 ], aluminum [ 84 ], and drought [ 85 ] stresses.…”

“…In addition to the cytochrome respiratory pathway common to all eukaryotes, plants contain the cyanide-insensitive alternative respiratory pathway catalyzed by the alternative oxidase (AOX) [ 49 , 50 , 51 ]. Several studies have reported the involvement of AOX in the neutralization of an excessive amount of ROS, as well as the critical role of alternative respiratory pathway in the adaptation of plants to different stresses [ 52 , 53 ]. Thus, cyanide-insensitive respiration was shown to be involved in plant protection against thermogenesis in the flowering of the sacred lotus [ 54 ] and Araceae plants [ 55 ].…”

Reactive Oxygen Species and Salicylic Acid Mediate the Responses of Pear to Venturia nashicola Infection

“…In the present study, we found that following V. nashicola infection, a much larger increase in the MDA content occurred in the susceptible cultivar Xuehua than in the resistant cultivar Huangguan. MDA is one of the major products of membrane lipid peroxidation [34]. The V. nashicola-induced increase in MDA indicates that damage to the cell membrane system of pear leaves was more serious in Xuehua than in Huangguan.…”

Employing CRISPR/Cas Technology for the Improvement of Potato and Other Tuber Crops

“…A CRISPR vector for the functional gene StAOX of the potato cyanide-resistant respiratory pathway was designed to examine the role of cyanide-resistant respiration in potato against high-light stress. The results indicated that high-light stress induced expression of AOX and accelerated cyanide-resistant respiration, and that loss of StAOX directly led to inhibition of cyanide-resistant respiration ( Hua et al, 2020 ).…”