Employing CRISPR/Cas Technology for the Improvement of Potato and Other Tuber Crops

Tussipkan, Dilnur; Manabayeva, Shuga A.

doi:10.3389/fpls.2021.747476

Cited by 20 publications

(8 citation statements)

References 142 publications

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“…Based on this, scientists can further target genes directly associated with pathogen resistance, such as genes encoding disease resistant proteins or regulatory factors involved in plant immune responses. By precisely knocking out, modifying, or introducing new gene fragments, new varieties that can resist specific diseases can be created, such as those that can effectively combat cassava mosaic virus or African cassava mosaic virus (Tussipkan and Manabayeva, 2021).…”

Section: Application Examples Of Crispr/cas Technology In Cassava Dis...mentioning

confidence: 99%

Genetic Mechanism of Cassava Disease Resistance: From Traditional Breeding to CRISPR/Cas Application

Huang,

Hong

2024

gab

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The escalating threat of plant diseases to cassava (Manihot esculenta) production necessitates innovative strategies for developing disease-resistant varieties. Traditional breeding has been instrumental in enhancing cassava's resistance to various pathogens, but it is often limited by the complexity of genetic traits and the lengthy timeframes required. The advent of CRISPR/Cas genome editing technology has revolutionized the field of plant breeding by enabling precise modifications of plant genomes. This systematic review provides a comprehensive analysis of the genetic mechanisms underlying cassava disease resistance and the transition from conventional breeding techniques to the cutting-edge CRISPR/Cas applications. We examine the current state of knowledge on plant-pathogen interactions in cassava and discuss how CRISPR/Cas-mediated genome editing has been employed to disrupt these interactions by targeting susceptibility factors within the plant genome. Furthermore, we explore the advancements in genome editing tools, such as base editing and prime editing, that have broadened the scope of generating disease-resistant cassava varieties. The review also highlights the potential of CRISPR/Cas9 in enhancing disease resistance through multiplexed gene editing and trait stacking, which is particularly relevant for complex traits like disease resistance. By synthesizing insights from recent developments in CRISPR/Cas applications across various crops, we aim to provide a roadmap for future research and the development of cassava varieties with improved resistance to a spectrum of diseases, thereby contributing to global food security.

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Section: Application Examples Of Crispr/cas Technology In Cassava Dis...mentioning

confidence: 99%

Genetic Mechanism of Cassava Disease Resistance: From Traditional Breeding to CRISPR/Cas Application

Huang,

Hong

2024

gab

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“…This tool exploit is designed to guide RNAs that identify a protospacer adjacent motif (PAM) sequence occurring downstream of the target-DNA [ 41 ]. The CRISPR/Cas 9 system was successfully implemented to engineer genomes of various plants, including crops showing resistance to environmental stresses, nutrient content enrichment, and yield improvement [ 42 ]. Most recently, the CRISPR/Cas9 strategy was used to edit DCL2 and DCL4 genes dicer-like in N. benthamiana plants.…”

Section: Molecular Tools For Therapeutic Protein Production In Crop P...mentioning

confidence: 99%

Compendium on Food Crop Plants as a Platform for Pharmaceutical Protein Production

Gerszberg

Hnatuszko-Konka

2022

IJMS

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Tremendous advances in crop biotechnology related to the availability of molecular tools and methods developed for transformation and regeneration of specific plant species have been observed. As a consequence, the interest in plant molecular farming aimed at producing the desired therapeutic proteins has significantly increased. Since the middle of the 1980s, recombinant pharmaceuticals have transformed the treatment of many serious diseases and nowadays are used in all branches of medicine. The available systems of the synthesis include wild-type or modified mammalian cells, plants or plant cell cultures, insects, yeast, fungi, or bacteria. Undeniable benefits such as well-characterised breeding conditions, safety, and relatively low costs of production make plants an attractive yet competitive platform for biopharmaceutical production. Some of the vegetable plants that have edible tubers, fruits, leaves, or seeds may be desirable as inexpensive bioreactors because these organs can provide edible vaccines and thus omit the purification step of the final product. Some crucial facts in the development of plant-made pharmaceuticals are presented here in brief. Although crop systems do not require more strictly dedicated optimization of methodologies at any stages of the of biopharmaceutical production process, here we recall the complete framework of such a project, along with theoretical background. Thus, a brief review of the advantages and disadvantages of different systems, the principles for the selection of cis elements for the expression cassettes, and available methods of plant transformation, through to the protein recovery and purification stage, are all presented here. We also outline the achievements in the production of biopharmaceuticals in economically important crop plants and provide examples of their clinical trials and commercialization.

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“…Genome editing systems, such as CRISPR/Cas, have been used for trait improvement of tubers, fibers, and cereal crops (Gao et al, 2017;Liu et al, 2021;Tussipkan and Manabayeva 2021). To our knowledge, there are few studies on using the CRISPR/Cas9 system for the enhancement of phytoremediation efficiency.…”

Section: Genome Editing Plants For Phytoremediationmentioning

confidence: 99%

Alfalfa (Medicago Sativa L.): Genotypic Diversity and Transgenic Alfalfa for Phytoremediation

Tussipkan

Manabayeva

2022

Front. Environ. Sci.

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Soil contamination caused by industrial and agricultural activities is an environmental problem that poses a serious risk to human health and the ecosystem. Persistent organic pollutants (POPs) are organic chemicals that persist in the environment for long periods because of their high resistance to photolytic, chemical, and biological degradation. Besides POPs, high concentrations of non-essential heavy metals and metalloids, such as arsenic, cadmium, and lead, are increasingly becoming a problem worldwide. Remediation strategies for organic and inorganic pollutants in the environment have received global attention. For organic or inorganic contaminants, phytoremediation is the strategy of choice because of a green technology that uses plants and solar energy to clean hyper-accumulated toxic pollutants from the environment. Some plant species have a high capacity to grow and survive in elevated levels of contaminants. With a long cultivation history and adaptability to a wide range of territories, alfalfa has not only widely been used for animal feed and a medicinal herb but is also an ideal natural resource and model plant for remediation of contaminated soils, offering a variety of elite characteristics. This review provides, firstly, abundant genomic information on the genetic diversity and population structure of alfalfa. Secondly, we focused on the transgenic alfalfa plants for enhanced phytoremediation of POPs, such as atrazine, polychlorinated biphenyl (PCB), and trichloroethylene (TCE), as well as phytoremediation of petroleum and heavy metals. Thirdly, the future perspective of enhancement of phytoremediation efficiency was discussed in depth. This review is intended to provide a comprehensive overview of the phytoremediation capabilities of transgenic alfalfa plants, presenting fundamental information for future research studies for enhancing phytoremediation efficiency.

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Employing CRISPR/Cas Technology for the Improvement of Potato and Other Tuber Crops

Cited by 20 publications

References 142 publications

Genetic Mechanism of Cassava Disease Resistance: From Traditional Breeding to CRISPR/Cas Application

Genetic Mechanism of Cassava Disease Resistance: From Traditional Breeding to CRISPR/Cas Application

Compendium on Food Crop Plants as a Platform for Pharmaceutical Protein Production

Alfalfa (Medicago Sativa L.): Genotypic Diversity and Transgenic Alfalfa for Phytoremediation

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