The Role of Curcuma Longa Rhizomes Ethanolic Extract on Human Lymphocytes Treated by Bickel by Using G Banding Technique and Karyotyping

Abstract

Curcuma longa L., which belongs to the Zingiberaceae family, is a perennial herb distributed throughout tropical and subtropical regions of the world, being widely cultivated in Asiatic countries, Curcuminoids are inherent compounds of the species C. longa, there are three main compounds of this pigmented curcuminoid complex, namely, curcumin, demethoxycurcumin, and bisdemethoxycurcumin. Curcumin is the major component of this plant, being responsible for the activities. Crude extract of Curcuma longa rhizomes was prepared by using ethanol and Chemical detection was performed in order to investigate the secondary metabolites compounds of ethanolic crude extract. Reversed high performance liquid chromatography carried out for the detection and estimation of curcumin in the crude extract. Otherwise cytogenetic study of the effect of C. longa crude extract and standard curcumin on the normal human lymphocyte blood cells done after exposure to nickel chloride (NiCl2), furthermore, karyotyping was performed by using MetasystemIkaros. Chemical detection of crude extract showed positive result for alkaloids, tannins, glycosides, phenols, Terpens, flavonoids, cumarines and resins while showed negative result for Saponins and Steroids. While the results of the cytogenetic study revealed that the effect of C. longa crude extract and standard curcumin reduced the genotoxic effect of nickel chloride (NiCl2) on the normal human lymphocyte blood cells. Karyotyping showed that genotoxic effect of nickel chloride appeared as aberration in chromosome number 18. While lymphocytes treated with C. longa crude extract or standard curcumin showed no chromosomal aberration, at the same time no chromosomal aberration were observed after treatment with combination of NiCl2 and crude extract or combination of NiCl2 and standard curcumin which indicated the ability of standard curcumin and C. longa crude extract to prevent the effect of nickel chloride. Further medical cytogenetic and molecular testing to get better understanding the protective role of Curcuma longa rhizomes, crude extract against heavy metal induced chromosomal aberrations in human blood cultures.

Curcumin assisted green synthesis of silver and zinc oxide nanostructures and their antibacterial activity against some clinical pathogenic multi-drug resistant bacteria

“…In the present study, the extraction process of curcumin from the Curcuma longa plant was carried out by following the previously reported extraction protocol by Pothitirat and Gritsanapan 35 with a bit of modification. 36,37 Briefly, about 100 g of Curcuma longa powder was mixed in 1000 ml of absolute ethanol under vigorous stirring and reflux at 60C for 24 h. Then the mixture was filtered using Whatman filter paper. The net yield of curcumin extracted from Curcuma longa powder was calculated by obtaining the precipitate from the extraction process was dried at room temperature and then weighted to obtain the yield of extracted curcumin as follows;Extracted curcumin (g) = the initial used Curcuma longa – the obtained precipitate powder…”

Anticancer potential of turmeric (Curcuma longa) ethanol extract and prediction of its mechanism through the Akt1 pathway

“…The filtrate was evaporated using a rotary evaporator (Buchi R-114) at a temperature of 50-60°C. [20][21][22] Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis LC-HRMS analysis was conducted at Central Laboratory of Life Sciences, Brawijaya University. The ethanol extract of turmeric was dissolved in dimethyl sulfoxide (DMSO) (Merck) to make a final volume of 1300 μL.…”