The role of carboxyl, guanidine and imidazole groups in catalysis by a midgut trehalase purified from an insect larvae

Antioxidant defenses preserve membrane transport activity in Chironomus riparius larvae exposed to anoxia

“…In order to utilize blood trehalose, insects possess an enzyme, α,α-trehalase, which catalyzes the hydrolysis of 1 molecule of trehalose into 2 molecules of glucose. Trehalase is found both in soluble and particulate forms bound to plasma membranes on the blood side (Thompson, 2003;Silva et al, 2004;Mitsumasu et al, 2005). No comparative information is currently available from the literature on the activity of these forms during oxygen deficiency.…”

Molecular Cloning of cDNA for Trehalase from the European Honeybee,Apis melliferaL., and Its Heterologous Expression inPichia pastoris

“…In rat intestinal trehalase, carboxy and thiol groups involved in the catalytic reaction have been suggested by the kinetic analysis of the interaction between trehalase and various inhibitors; 37) in trehalase of lady beetle Harmonia axyridis, a histidine residue, by modification of the enzyme; 38) and in trehalase of moth larvae, Spodoptera frugiperda, an Arg residue, by pK a and inhibition experiments. 39) As shown in Fig. 3, however, His or Cys residue is not conserved in GH37 enzymes, while some Arg residues are conserved (Arg181, 228, 234, and 339 in honeybee trehalase).…”

Molecular cloning and expression analysis of soluble and membrane-bound trehalase genes in the cotton bollworm, Helicoverpa armigera

“…Soluble trehalase is a cytosolic enzyme involved in the hydrolysis of endogenous trehalose, while membrane-bound trehalase is an extracellular enzyme thought to have a role in the assimilation of exogenous trehalose as a carbon source (de Almeida et al, 2009;Chen et al, 2010). Trehalase was believed to be located on the plasma membrane on the hemolymph side or within the cytoplasm in most tissues, where glucose-related energy metabolism occurs (Becker et al, 1996;Silva et al, 2004;Mitsumasu et al, 2005). Due to its importance in insect physiology, trehalase is also a good target for insect control and studies of this enzyme could lead to new pest control strategies (Asano, 2003;Chen et al, 2010;Bansal et al, 2013).…”