Molecular Cloning of cDNA for Trehalase from the European Honeybee,Apis melliferaL., and Its Heterologous Expression inPichia pastoris

Catalytic Reaction Mechanism Based on α-Secondary Deuterium Isotope Effects in Hydrolysis of Trehalose by European Honeybee Trehalase

“…In our previous studies, 4,20) the bound-type trehalase was purified from European honeybees, and the kinetic properties of the hydrolytic reaction, molecular cloning of the cDNA, and the gene structure in chromosome were examined.…”

cDNA cloning and characterization of two trehalases from Spodoptera litura (Lepidoptera; Noctuidade)

“…To date, 2 forms of trehalase have been found and cloned in several insect species (Wyatt, 1967), including Apis mellifera (Lee et al, 2007), Bombyx mori (Mitsumasu et al, 2005), Omphisa fuscidentalis (Tatun et al, 2008), Pimpla hypochondriaca (Parkinson et al, 2003), Spodoptera exigua (Tang et al, 2008), and Tenebrio molitor (Takiguchi et al, 1992). Initial studies of trehalose and protein purification have found that the main distribution of soluble trehalase (Treh1) and membrane-bound trehalase (Treh2) sometimes differed (Tang et al, 2008), which might indicate different functions.…”

Molecular cloning and expression analysis of soluble and membrane-bound trehalase genes in the cotton bollworm, Helicoverpa armigera

“…The first insect trehalase gene, a soluble trehalase gene from Tenebrio molitor was reported in 1992 (Takiguchi et al, 1992), but a membrane-bound trehalase gene was not reported until 2005 (Mitsumasu et al, 2005). So far, two forms of trehalases have been found and cloned in many insect species, including Apis mellifera (Lee et al, 2007), Aphis glycines (Bansal et al, 2013), B. mori (Mitsumasu et al, 2005), O. fuscidentalis (Tatun et al, 2008), P. hypochondriaca (Parkinson et al, 2003), S. exigua (Tang et al, 2008) and S. litura (Zou et al, 2013). In this study, we successfully cloned the two types of trehalase genes in H. armigera, and the analysis of the deduced amino acid sequence demonstrated that HaTreh-1 and HaTreh-2 coded for soluble and membrane-bound trehalase, respectively.…”

“…Despite the importance of trehalase in insects, genes encoding Trehs have been cloned and characterized in only a few species. To date, two forms of trehalase have been cloned and/or characterized in species belonging to Hemiptera, Coleoptera, Lepidoptera, Diptera and Hymenoptera (Takiguchi et al, 1992;Mitsumasu et al, 2005;Lee et al, 2007;Tang et al, 2008;Tatun et al, 2008b;Gu et al, 2009;Silva et al, 2009;Bansal et al, 2013;Zou et al, 2013;Wang et al, 2014). Within lepidopteran insects, Trehs have been studied in Bombyx mori (Yamashita et al, 1974;Sumida and Yamashita, 1977;Azuma and Yamashita, 1985a,b;Mitsumasu et al, 2005), Spodoptera exigua (Tang et al, 2008;Chen et al, 2010), Spodoptera litura (Zou et al, 2013) and Spodoptera frugiperda ).…”