The Role of Buffers in Wild-Type HEWL Amyloid Fibril Formation Mechanism: A Methodological Approach

Brudar, Sandi; Hribar-Lee, Barbara

doi:10.1007/978-1-0716-2597-2_19

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Similar curves have been observed in other studies of HEWL fibrils [ 50 , 51 ]. In literature, many different protocols of fibril growing can be found [ 52 , 53 , 54 ]. The exact kinetic parameters of fibril formation are heavily dependent on experimental conditions, such as temperature, protein concentration, stirring rate, or the presence of other substances [ 55 , 56 ].…”

Section: Resultsmentioning

confidence: 99%

The Ability of Some Polysaccharides to Disaggregate Lysozyme Amyloid Fibrils and Renature the Protein

et al. 2023

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The deposition of proteins in the form of insoluble amyloid fibril aggregates is linked to a range of diseases. The supramolecular architecture of such deposits is governed by the propagation of β-strands in the direction of protofilament growth. In the present study, we analyze the structural changes of hen egg-white lysozyme fibrils upon their interactions with a range of polysaccharides, using AFM and FTIR spectroscopy. Linear anionic polysaccharides, such as κ-carrageenan and sodium alginate, are shown to be capable to disaggregate protofilaments with eventual protein renaturation. The results help to understand the mechanism of amyloid disaggregation and create a platform for both the development of new therapeutic agents for amyloidose treatment, and the design of novel functional protein–polysaccharide complex-based nanomaterials.

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Section: Resultsmentioning

confidence: 99%

The Ability of Some Polysaccharides to Disaggregate Lysozyme Amyloid Fibrils and Renature the Protein

et al. 2023

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“…That means BM protein extract is increasing the stability of the HEWL to protect it from the chemical denaturation caused by GuHCl as higher concentration of Gu-Hcl is needed to denature half of the HEWL proteins when BM protein extract is added. CONCLUSION: Lysozyme amyloidosis has been studied in different contexts and proven to be a very good model system to identify novel molecules of interest from natural and synthetic sources 5,[20][21][22][23][24] . A comparative study from three medicinal plants showed that BM leaf protein extract can inhibit lysozyme fibrillation.…”

Section: Dtt-induced Aggregation Is Inhibited In the Presence Of Bm L...mentioning

confidence: 99%

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2023

IJPSR

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Lysozyme amyloid formation is studied as model in-vitro system to find out natural or synthetic inhibitors of amyloidosis. Protein aggregation and amyloid formation is the basic reason for many neurodegenerative diseases. Indigenous medicinal plants are great source of therapeutic molecules and drugs. Ethnobotanical studies have indicated many plants have neuroprotective functions. We have studied three indigenous medicinal plant namely Bacopa monnieri (BM), Trigonella foenum-graecum (TFG) and Tridax procumbens (TP) showing various pharmacological properties as inhibitors of lysozyme aggregation. We isolated leaf proteins and did the protein aggregation studies. Anti-aggregation properties of leaf extract were carried out by following assays -DTT induced lysozyme aggregation assay, Congo red binding Assay, Thiaflavin binding assay and protein stability assay by Tryptophan Fluorescence. BM leaf protein extract showed maximum inhibition followed by Methi (TFG) and then TP.

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Wheat gluten amyloid fibrils: Conditions, mechanism, characterization, application, and future perspectives

Zhang,

Lv,

Jin

et al. 2023

International Journal of Biological Macromolecules

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The Role of Buffers in Wild-Type HEWL Amyloid Fibril Formation Mechanism: A Methodological Approach

Cited by 3 publications

References 12 publications

The Ability of Some Polysaccharides to Disaggregate Lysozyme Amyloid Fibrils and Renature the Protein

The Ability of Some Polysaccharides to Disaggregate Lysozyme Amyloid Fibrils and Renature the Protein

Untitled

Wheat gluten amyloid fibrils: Conditions, mechanism, characterization, application, and future perspectives

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