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Abstract

Lysozyme amyloid formation is studied as model in-vitro system to find out natural or synthetic inhibitors of amyloidosis. Protein aggregation and amyloid formation is the basic reason for many neurodegenerative diseases. Indigenous medicinal plants are great source of therapeutic molecules and drugs. Ethnobotanical studies have indicated many plants have neuroprotective functions. We have studied three indigenous medicinal plant namely Bacopa monnieri (BM), Trigonella foenum-graecum (TFG) and Tridax procumbens (TP) showing various pharmacological properties as inhibitors of lysozyme aggregation. We isolated leaf proteins and did the protein aggregation studies. Anti-aggregation properties of leaf extract were carried out by following assays -DTT induced lysozyme aggregation assay, Congo red binding Assay, Thiaflavin binding assay and protein stability assay by Tryptophan Fluorescence. BM leaf protein extract showed maximum inhibition followed by Methi (TFG) and then TP.