The role of Bacillus acidophilus in osteoporosis and its roles in osteocyte proliferation and differentiation

Abstract

The microbes play an important role to physiological activities of human. Osteoporosis is one of the most closely relationship diseases with the elderly. In recent years, the studies found that gut microbiota can cause osteoporosis. These microbes may affect activity of osteoclasts and osteoblasts to affect bone metabolism. We selected 5 health people and 10 osteoporosis patients and found that the diversity of gut microbiota in osteoporosis patients are decreased and imbalance with lower abundance of lactobacillus and butylic acid bacteria, meanwhile, 25-hydroxyitamin D and procollagen type I N-terminal peptide (PINP) of osteoporosis patient was significantly lower than the normal group. The proliferation, differentiation and maturity of osteoblasts MC3T3-E1 cells was stimulated, the activity of alkaline phosphatase, concentration of osteocalcin and the expression of RUNX2, WNT2 and CTNNB1 was improved by supernatant of lactobacillus acidophilus and butanoic acids, however, the proliferation, differentiation and maturity, the expression of RANK,WNT2 and CTNNB1 in osteoclasts RAW 264.7 cells was suppressed. There is not a similar significant effect by lactobacillus rhamnosus treatment.

Exploring the bone sparing effects of postbiotics in the post-menopausal rat model

“…Rahman et al exhibited that conjugated linoleic acid inhibits osteoclastogenesis by modulating RANKL signaling [ 48 ]. Chen et al revealed that the supernatant of Lactobacillus acidophilus and butanoic acids stimulated the proliferation, differentiation, and maturity of osteoblasts MC3T3-E1 cells was, increased the activity of alkaline phosphatase, elevated concentration of osteocalcin, and the expression of RUNX2, WNT2 and CTNNB1 [ 49 ].…”

Exploring the bone sparing effects of postbiotics in the postmenopausal rat model

“…Rahman et al exhibited that conjugated linoleic acid inhibits osteoclastogenesis by modulating RANKL signaling (42). Chen et al revealed that the supernatant of Lactobacillus acidophilus and butanoic acids stimulated the proliferation, differentiation, and maturity of osteoblasts MC3T3-E1 cells was, increased the activity of alkaline phosphatase, elevated concentration of osteocalcin, and the expression of RUNX2, WNT2 and CTNNB1 (43).…”