The Role of an Anaerobic Stage on Biological Phosphorus Removal

Fukase, T.; Shibata, Masahide; Miyaji, Y.

doi:10.2166/wst.1985.0120

Cited by 66 publications

(33 citation statements)

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“…GAOs were shown to dominate laboratory-scale cultures fed with acetate (12,48,72) and usually are associated with the deterioration of EBPR systems (46). Some operational factors, such as a long SRT (27), excessive aeration (7), high temperatures (39), and a low phosphorus/carbon ratio in the feed (36,37) are described in literature and favor growth of GAOs over PAOs. In our study, the dominance of GAOs at the expense of decreasing numbers of PAOs was directly related to the increase in salt concentration.…”

Section: Effect Of Increasing Nacl Concentrations On Pao-gao Competitmentioning

confidence: 99%

Effect of Elevated Salt Concentrations on the Aerobic Granular Sludge Process: Linking Microbial Activity with Microbial Community Structure

Bassin

Pronk

Muyzer

et al. 2011

Appl Environ Microbiol

168

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The long-and short-term effects of salt on biological nitrogen and phosphorus removal processes were studied in an aerobic granular sludge reactor. The microbial community structure was investigated by PCR-denaturing gradient gel electrophoresis (DGGE) on 16S rRNA and amoA genes. PCR products obtained from genomic DNA and from rRNA after reverse transcription were compared to determine the presence of bacteria as well as the metabolically active fraction of bacteria. Fluorescence in situ hybridization (FISH) was used to validate the PCR-based results and to quantify the dominant bacterial populations. The results demonstrated that ammonium removal efficiency was not affected by salt concentrations up to 33 g/liter NaCl. Conversely, a high accumulation of nitrite was observed above 22 g/liter NaCl, which coincided with the disappearance of Nitrospira sp. Phosphorus removal was severely affected by gradual salt increase. No P release or uptake was observed at steady-state operation at 33 g/liter NaCl, exactly when the polyphosphate-accumulating organisms (PAOs), "Candidatus Accumulibacter phosphatis" bacteria, were no longer detected by PCR-DGGE or FISH. Batch experiments confirmed that P removal still could occur at 30 g/liter NaCl, but the long exposure of the biomass to this salinity level was detrimental for PAOs, which were outcompeted by glycogen-accumulating organisms (GAOs) in the bioreactor. GAOs became the dominant microorganisms at increasing salt concentrations, especially at 33 g/liter NaCl. In the comparative analysis of the diversity (DNA-derived pattern) and the activity (cDNA-derived pattern) of the microbial population, the highly metabolically active microorganisms were observed to be those related to ammonia (Nitrosomonas sp.) and phosphate removal ("Candidatus Accumulibacter").Numerous wastewaters, like those generated in seafood canning, pickling, and cheese-processing industries, can contain significant amounts of inorganic dissolved salts (14). The use of seawater for toilet flushing in ships, offshore installations, and regions with problems related to water supply also introduces salts into freshwater. Wastewaters with a high salt content require the activity of salt-tolerant microorganisms which may not be present in high numbers in microbial inocula from sewage treatment plants. Salt ions exert high osmotic pressure on the microorganisms, and most of the freshwater-based microbial populations are unable to survive at these high osmotic pressures and either die or become dormant under these conditions (40). Besides that, inorganic salts can affect the structure and settling properties of microbial flocs as well as the maximum solubility of oxygen and its transfer to the liquid phase, which can lead to oxygen limitation. Consequently, high salt concentrations have a negative influence on existing biological wastewater treatment plants, affecting organic matter, nitrogen, and phosphorus removal (17). So far, most studies on chemical oxygen demand (COD), N, and P removal from saline wastewa...

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Section: Effect Of Increasing Nacl Concentrations On Pao-gao Competitmentioning

confidence: 99%

Effect of Elevated Salt Concentrations on the Aerobic Granular Sludge Process: Linking Microbial Activity with Microbial Community Structure

Bassin

Pronk

Muyzer

et al. 2011

Appl Environ Microbiol

168

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“…Microbiol. ; Fukase et al, 1984;Liu et al, 1994;Satoh et al, 1994). In P-removing sludge it is thought the ability of PAO to take up volatile fatty acids (VFA) in the anaerobic stage provides the selective advantage for these bacteria in EBPR reactors.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic phosphate release from activated sludge with enhanced biological phosphorus removal. A possible mechanism of intracellular pH control

Bond

Keller

Blackall

1999

Biotechnol. Bioeng.

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“…The anaerobic reactor was an excellent zone for material exchange. Organic carbon source was offered to microorganisms and phosphorus was released into liquid by phosphorus accumulating organisms [12]. The sludge released phosphorus which was absorbed from aerobic zone to the supernatant liquid in anaerobic zone, and then it was delivered back to the MBR.…”

Section: Resultsmentioning

confidence: 99%

Phosphorus removal and recovery from domestic wastewater in a membrane bioreactor with external recycle anaerobic process of sludge

Feng

Wang

et al. 2010

2010 International Conference on Mechanic Automation and Control Engineering

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The removal of phosphorus from domestic wastewater in a membrane bioreactor with external recycle anaerobic process of sludge was investigated. More than 85% of phosphorus removal efficiency could be achieved steadily with external recycle of sludge. In the anaerobic reactor, the sufficiency of phosphorus released from the phosphorus-enriched sludge was an important factor for phosphorus removal from the system. Sodium acetate as the external carbon source was much better than the real domestic wastewater for the release of phosphorus during the anaerobic process. And the release quantities of phosphorus increased with improving the dose of sodium acetate in some extent. The experimental data showed that one millimole phosphorus releasing required about 1.048mmol sodium acetate. Moreover, the recovery of phosphorus in the supernatant was also studied by batch tests. Under the conditions of [Mg 2+ ]:[NH 3 -N]:[P] =2:2:1 and the initial phosphorus concentration of 49.0mg/L, the optimal pH value was about 9.0 considering the struvite as the dominant product. Although increasing pH value could improve the phosphorus recovery efficiency, the precipitate transformed from struvite to magnesium phosphate as the pH value above 9.0. During struvite precipitation process, increasing the concentration of NH 3 -N to improve struvite production was more suitable than increasing the dose of [Mg 2+ ]. In addition, calcium ions in the domestic wastewater had competition effect on struvite precipitation.

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The Role of an Anaerobic Stage on Biological Phosphorus Removal

Cited by 66 publications

References 0 publications

Effect of Elevated Salt Concentrations on the Aerobic Granular Sludge Process: Linking Microbial Activity with Microbial Community Structure

Effect of Elevated Salt Concentrations on the Aerobic Granular Sludge Process: Linking Microbial Activity with Microbial Community Structure

Anaerobic phosphate release from activated sludge with enhanced biological phosphorus removal. A possible mechanism of intracellular pH control

Phosphorus removal and recovery from domestic wastewater in a membrane bioreactor with external recycle anaerobic process of sludge

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