The RNA Processing Enzyme Polynucleotide Phosphorylase Negatively Controls Biofi lm Formation by Repressing Poly-N-Acetylglucosamine (PNAG) Production in Escherichia coli C

Sayen, Stéphanie

doi:10.1201/b16593-8

Cited by 12 publications

(19 citation statements)

References 49 publications

(66 reference statements)

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“…Furthermore, the observed Rr values indicate similar 'carrying capacities' of the biofilms during all seasons, which are comparable with the findings of Lubarsky et al (2012). Moreover, the complexity of biostabilisation was demonstrated by the fact that the seasonal changing biofilm stability was not mirrored by seasonal differences in microbial biomass.…”

Section: Factors Driving Biofilm Growth and Biostabilisationsupporting

confidence: 83%

The effect of seasonality upon the development of lotic biofilms and microbial biostabilisation

Schmidt

Thom

King³

et al. 2016

Freshwater Biology

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Summary Fine sediments (fine sands or silts) are significantly impacted by microbial biostabilisation. This process complicates precise modelling solutions for sediment dynamics and management strategies for riverine sediment. The present publication investigates the effect of seasonality upon biostabilisation. In total, five straight flume experiments with fluvial water were performed during spring, summer and autumn under identical boundary conditions. The extracellular polymeric substances (EPS), microbial biomass and microbial community composition of the developing biofilms were analysed. In addition, biofilm adhesiveness was measured. Highest biostabilisation occurred during spring (up to six times greater than during autumn) and coincided with maximal EPS production – especially extracellular proteins indicating the essential role of adhesive proteins for the stability of the biofilm matrix. Furthermore, not biomass but microbial community composition significantly differed between seasons. For instance, during minimal biostabilszation in autumn, the diatom community was dominated by the motile diatoms Nitzschia fonticola and Nitzschia dissipata var. dissipata. Concurrently, the highest rates of change within the bacterial community were detected. This suggests a disruptive impact of diatom movement upon the biofilm matrix and overall biofilm stability. These findings emphasise the importance of detailed analyses of the microbial community and demonstrate the complex interactions of distinct biofilm features influencing the functionality of the overall biofilm system.

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Section: Factors Driving Biofilm Growth and Biostabilisationsupporting

confidence: 83%

The effect of seasonality upon the development of lotic biofilms and microbial biostabilisation

Schmidt

Thom

King³

et al. 2016

Freshwater Biology

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“…Proia et al suggested that the triclosan effects on algae could be indirect effects resulting from triclosan toxicity to bacteria. However, this suggestion is contradicted by Lawrence et al , who showed that 35 nM of triclosan caused freshwater periphyton to become more heterotrophic.…”

Section: Resultsmentioning

confidence: 90%

Long‐term effects of the antibacterial agent triclosan on marine periphyton communities

Eriksson

Johansson

Fihlman

et al. 2015

Enviro Toxic and Chemistry

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Triclosan is a widely used antibacterial agent that has become a ubiquitous contaminant in freshwater, estuary, and marine environments. Concerns about potential adverse effects of triclosan have been described in several recent risk assessments. Its effects on freshwater microbial communities have been well studied, but studies addressing effects on marine microbial communities are scarce. In the present study, the authors describe short- and long-term effects of triclosan on marine periphyton (microbial biofilm) communities. Short-term effects on photosynthesis were estimated after 60 min to 210 min of exposure. Long-term effects on photosynthesis, chlorophyll a fluorescence, pigment content, community tolerance, and bacterial carbon utilization were studied after exposing periphyton for 17 d in flow-through microcosms to 0.316 nM to 10,000 nM triclosan. Results from the short-term studies show that triclosan is toxic to periphyton photosynthesis. Half maximal effective concentration (EC50) values of 1080 nM and 3000 nM were estimated using (14)CO2-incorporation and pulse amplitude modulation (PAM) fluorescence measurements, respectively. After long-term triclosan exposure in flow-through microcosms, photosynthesis estimated using PAM fluorometry was not inhibited by triclosan concentrations up to 1000 nM but instead increased with increasing triclosan concentration. Similarly, at exposure concentrations of 31.6 nM and higher, triclosan caused an increase in photosynthetic pigments. At 316 nM triclosan, the pigment amounts were increased by a factor of 1.4 to 1.9 compared with the control level. Pollution-induced community tolerance was observed for algae and cyanobacteria at 100 nM triclosan and higher. Despite the widespread use of triclosan as an antibacterial agent, the compound did not have any effects on bacterial carbon utilization after long-term exposure.

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“…Previous studies have revealed the effect of the repeated exposure of sublethal concentrations of TCS on biofilm of bacteria (Lubarsky et al . ), lipid synthesis (Nielsen et al . ) and some resistance mechanisms like efflux pumps (Braoudaki and Hilton ).…”

Section: Discussionmentioning

confidence: 99%

Impact of triclosan adaptation on membrane properties, efflux and antimicrobial resistance of Escherichia coli clinical isolates

et al. 2019

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Aim Analysing the effect of step‐wise exposure of Escherichia coli isolates to sublethal concentrations of triclosan (TCS) that is widely used as an antiseptic, preservative and disinfectant. Methods and Results Changes in the tolerance to the biocide itself and the cross‐resistance to clinically important antibiotics were analysed. The involvement of efflux mechanism was studied as well as the possible implication of modifications in cytoplasmic membrane properties including integrity, permeability, potential and depolarization in the resistance mechanisms. Most of E. coli isolates that were adapted to TCS showed increased antimicrobial resistance, lower outer and inner membrane permeability, higher membrane depolarization, more negative membrane potential and enhanced efflux activity using qRT‐PCR. Nonsignificant change in membrane integrity was found in the adapted cells. Conclusion This study suggests that the extensive use of TCS at sublethal concentrations contributed to the emergence of antibiotic resistance in E. coli clinical isolates, by inducing changes in bacterial membrane properties and enhancing the efflux system. Significance and Impact of the Study The extensive usage of TCS has a deleterious effect on the spread of antibiotic resistance, and more studies are needed to investigate the molecular mechanisms underlying the effects of TCS.

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The RNA Processing Enzyme Polynucleotide Phosphorylase Negatively Controls Biofi lm Formation by Repressing Poly-N-Acetylglucosamine (PNAG) Production in Escherichia coli C

Cited by 12 publications

References 49 publications

The effect of seasonality upon the development of lotic biofilms and microbial biostabilisation

The effect of seasonality upon the development of lotic biofilms and microbial biostabilisation

Long‐term effects of the antibacterial agent triclosan on marine periphyton communities

Impact of triclosan adaptation on membrane properties, efflux and antimicrobial resistance of Escherichia coli clinical isolates

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