The Rice Stripe Virus Group

The entire 1317 nucleotide sequence of the maize stripe virus (MStV) RNA5 was determined. Only one open reading frame (ORF) was identified and was found in the viral complementary RNA (vcRNA). This ORF appears to encode a protein ofM r 44237, hereafter referred to as NS 5. In vitro translation of transcripts representing nearly full-length RNA5 vcRNA yielded products of the predicted size, as well as some smaller, less prominent products. No products were identified from transcripts of viral polarity. RNA hybridization analyses of MStVinfected Zea mays revealed RNAs corresponding only to full-length RNA5, but both positive and negative polarity RNAs were abundant. Analysis of the NS 5 amino acid sequence revealed that it is extremely basic, containing 21% arginine and lysine. Database comparisons showed that NS 5 had no significant similarity to other protein sequences.

Section: Introductionmentioning

confidence: 99%

“…Filamentous ribonucleoprotein particles (RNPs) which contain four or five distinct RNAs can be isolated from tenuivirus-infected plants (Falk & Tsai, 1984;Falk et al, 1989;Gingery, 1988;Ishikawa et aL, 1989;Toriyama & Watanabe, 1989;Ramirez et al, 1992). The estimated sizes of these RNAs range from approx.…”

Section: Introductionmentioning

confidence: 99%

Maize stripe virus RNA5 is of negative polarity and encodes a highly basic protein

Huiet

Tsai²,

Falk³

1993

“…It is transmitted by the planthopper Sogatodes oryzicola Muir, and belongs to the tenuivirus group, which also includes (Gingery, 1987) rice stripe virus (RSV, the type member), maize stripe virus (MStV), European wheat striate mosaic virus and rice grassy stunt virus (RGSV).…”

Section: Introductionmentioning

confidence: 99%

“…Tenuiviruses exhibit properties that are quite different from those of other plant RNA viruses (Gingery, 1987). Some of these properties are: (i) virus particles appear as fine filaments 3 to 8 nm wide and of variable length that may adopt a circular configuration (Gingery et al, 1981 ;Toriyama, 1982;Morales & Niessen, 1983;Hibino et al, 1985); (ii) they are persistently transmitted by a delphacid planthopper in which they are also transovarially passed (Zeigler & Morales, 1990); (iii) a single species of nucleocapsid (NC) protein of 31K to 34K is part of the infectious nucleoprotein (Gingery et al, 1981 ;Toriyama, 1982;Morales & Niessen, 1983;Hibino et al, 1985); (iv) they induce the accumulation in infected plants of large amounts of a 16K to 21K non-structural (NS) protein, also designated non-capsid protein (Gin-1" Permanent address: Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San Jos6, Costa Rica.…”

Section: Introductionmentioning

confidence: 99%

Rice hoja blanca virus genome characterization and expression in vitro

Ramirez

Macaya

Calvert

et al. 1992

No information exists on the organization and mechanisms of expression of the genome of rice hoja blanca virus (RHBV), a member of the tenuivirus group, but here we describe the first steps in its characterization. RHBV contains four ssRNA and three dsRNA species, the sizes of which were estimated by native and denaturing gel electrophoresis. Hybridization analyses using 32p-labelled riboprobes of viral and viral complementary polarities showed that unequal amounts of the two polarities of at least the smallest RNA are present in the virion, and indicated that the dsRNA species contain the same information as the ssRNA species of corresponding size. Total RHBV RNA directs the synthesis of two major proteins of 23K and 21K in vitro. RNA3 directs the synthesis of a 23K protein designated NS3, and RNA4 of a 21K protein designated NS4. The NS4 protein corresponds to the non-structural protein that accumulates in RHBV-infected rice tissue. The nucleocapsid protein is not translated from either total RHBV RNA or any individual RHBV RNA in vitro.

“…MStpV has several properties in common with a group of other planthopper-borne viruses, including rice hoja blanca virus (RHBV) (Morales & Niessen, 1983), rice stripe virus (RSV) (Toriyama, 1982;Gingery et al, 1983), European wheat striate mosaic virus (Ammar et al, 1985) and rice grassy stunt virus (Hibino et al, 1985). These viruses have been tentatively grouped in the RSV group (Gingery, 1987).…”

Section: Introductionmentioning

confidence: 99%

Differences in Levels of Detection for the Maize Stripe Virus Capsid and Major Non-capsid Proteins in Plant and Insect Hosts

Falk

Tsai

Lommel

1987

SUMMARYAntisera to the Mr 32000 (32K) capsid and Mr 16500 (16"5K) major non-capsid proteins were used for immunological analyses of extracts from maize stripe virus (MStpV)-infected maize (Zea mays) plants and from inoculative Peregrinus maidis, the MStpV planthopper vector. The 32K protein was easily detected in extracts of both MStpV-infected plants and inoculative P. maidis by ELISA and by immunological analysis of Western blots. In a time course study, no 32K protein was detected in P. maidis until 8 days after the beginning of a 5 day acquisition access period on MStpVinfected plants. The percentage of MStpV-positive P. maidis increased with time indicating multiplication of MStpV in P. maMis. The 32K protein was detected only in individual P. maidis that also transmitted MStpV to plant hosts. The 16.5K protein was also detected in MStpV-infected plant hosts but not in extracts of groups or of individual MStpV-inoculative P. rnaidis. In vitro translation of MStpV virion RNAs in rabbit reticulocyte lysates showed that both the 32K and 16.5K proteins were present in the translation products. The ready detection of the MStpV-coded 32K protein in both plant and insects and detection of the 16.5K protein in only plant hosts is discussed.