The revised COLIPA in vitro UVA method

Abstract

A multicentred study derived from the COLIPA in vitro UVA method was performed to assess the influence of test conditions on UVA protection factor (UVAPF) values in terms of amplitude, reproducibility between laboratories and correlation with in vivo UVA results. Eight products with a range of in vivo UVAPF from three to 29 were used. Two different types of plates, namely high-roughness (5 μm) and low-roughness (2 μm) plates, were used with a different application rate for each (1.3 mg cm(-2) and 0.75 mg cm(-2) respectively). The UVR dose applied to both plate types followed the same principle as the original test (1.2 J. cm(-2) × UVAPF0). Strong, significant correlations between in vitro and in vivo UVAPF values were observed for both plate types (Pearson correlation > 0.9, P ≤ 0.01). The correlation and slope obtained with the low-roughness plates confirmed the previous results obtained by COLIPA. Across all laboratories, higher UVAPF values were obtained on the high-roughness plates (P < 0.01). Reproducibility of UVAPF values between laboratories was comparable between the two plate roughness values (low roughness, COV = 8%; high roughness, COV = 12%). Considering the in vitro/in vivo comparisons, a regression slope of 0.83 was observed for the low-roughness plates, in comparison with a value of 1.05 for the high-roughness plates. The accuracy of the method was improved, therefore, with the use of the high-roughness plates. With a constraint to recommend the use of only one plate type in the COLIPA UVA in vitro Test, the high-roughness plate was selected on an on-going basis to limit variability of results and to provide better accuracy with in vivo data.

In vitro photoprotection and antioxidant capacity of Sphagnum meridense extracts, a novel source of natural sunscreen from the mountains of Colombia

“…In vitro photoprotection efficacy was calculated according to the following parameters: UVB efficacy by estimating sun protection factor (SPF); UVA efficacy by UVAPF and UVA/UVB ratio; and critical wavelength (λ c ). Measurements were read six times for each sample [19][20][21].…”

Novel Nanostructured Lipid Carriers with Photoprotective Properties Made from Carnauba Wax, Beeswax, and Kenaf Seed Oil

“…The European Cosmetic and Perfumery Association (COLIPA) method was applied as reported by Moyal et al () to perform the test to determine the UV absorbing properties. The NLC sample (31.0 ± 0.5 mg cm −2 ) was pipetted out by using positive displacement automatic pipettes and applied to the roughened side of polymethylmethacrylate (PMMA) plates (HD6 with dimensions 50 mm × 50 mm) (HelioScreen Labs, France) as a number of small droplets of approximately the same volume were distributed evenly by using a covered fingertip over the plate surface.…”

Photostability study of commercial sunscreens submitted to artificial UV irradiation and/or fluorescent radiation

“…An application density of 0.75 mg/cm 2 is recommended for the 2 μm plates (for a total mass of 18.75 mg), and an application density of 1.3 mg/cm 2 for the 5 μm plates (total mass 28.7 mg) [33,36]. In this study, a higher amount of product was used for each plate application, with the purpose of facilitating the application process as well as reducing errors.…”

“…Each product was applied on a PMMA plate using a micropipette and spread with the aid of a latex finger cot; then the plates were dried for 15 min, in an environment with no incident light from either natural or artificial sources, prior to performing the first analysis as previously described [31,33]. A reference plate was also prepared by spreading 33 mg of glycerine on the roughened side of a PMMA plate.…”