The resveratrol-enriched rice DJ526 boosts motor coordination and physical strength

Chung, Hea-Jong; Sharma, Satya Priya; Kim, Hyeon Jin; Baek, Seong-Bum; Hong, Seong‐Tshool

doi:10.1038/srep23958

Cited by 21 publications

(30 citation statements)

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“…The tissue integrity and neurodegeneration of the brains and eyes of Drosophila were visualized after hematoxylin and eosin staining (H&E staining). The H&E staining procedure was performed according to a previously described method 46 .…”

Section: Methodsmentioning

confidence: 99%

Oligomeric α-synuclein-specific degradation by HtrA2/Omi to bestow a neuroprotective function

Chung

Mahm

Rahman

et al. 2018

Preprint

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13Running title: HtrA2/Omi to bestow a neuroprotective function Although the malfunction of HtrA2/Omi leads to Parkinson's disease (PD), the underlying 15 mechanism has remained unknown. Here, we showed that HtrA2/Omi specifically removed 16 oligomeric α-Syn but not monomeric α-Syn to protect oligomeric α-Syn-induced 17 neurodegeneration. Experiments using mnd2 mice indicated that HtrA2/Omi degraded oligomeric 18 α-Syn specifically without affecting monomers. Transgenic Drosophila melanogaster 19 experiments of the co-expression α-Syn and HtrA2/Omi and expression of genes individually also 20 confirmed that pan-neuronal expression of HtrA2/Omi completely rescued Parkinsonism in the α-21 Syn-induced PD Drosophila model by specifically removing oligomeric α-Syn. HtrA2/Omi 22 maintained the health and integrity of the brain and extended the life span of transgenic flies. 23 Because HtrA2/Omi specifically degraded oligomeric α-Syn, co-expression of HtrA2/Omi and α-24 Syn in Drosophila eye maintained a healthy retina, while the expression of α-Syn induced retinal 25degeneration. This work showed that the bacterial function of HtrA to degrade toxic misfolded 26 proteins is evolutionarily conserved in mammalian brains as HtrA2/Omi. 27 Parkinson's disease (PD) is one of the most common neurodegenerative diseases, characterized by 29 the progressive loss of dopaminergic neurons in the substantia nigra of the central nervous system 1 . 30The degeneration of the dopaminergic neurons of the substantia nigra results in clinical 31 manifestations such as motor impairments, which involve resting tremor, bradykinesia, postural 32 instability, gait difficulty and rigidity 2 . Although the degeneration of dopaminergic neurons 33 directly leads to the clinical manifestations of PD, the pathogenic mechanism underlying the 34 degeneration of dopaminergic neurons at the molecular level is still unclear. The most manifested 35 pathophysiological feature of dopaminergic neurons of PD is an abnormal accumulation of 36 oligomeric α-Synuclein (α-Syn) in the form of Lewy bodies and Lewy neuritis inside neurons, 37 which represent the major hallmarks of PD 3 . α-Syn is a 140-a.a. presynaptic protein that plays an 38 important role in maintaining a supply of synaptic vesicles in presynaptic terminals 4, 5 . α-Syn 39 performs its normal biological function in neurons if present as a monomer. However, the 40 monomeric form of α-Syn is naturally prone to adopt a β-sheet conformation to form oligomeric 41 aggregates 6 . The oligomeric α-Syn has very strong neurotoxicity such that the aggregation plays a 42 causative role in dopaminergic neuronal degeneration 7, 8 . 43Since the accumulation of misfolded α-Syn is key to the pathology of PD, the question of how 44 misfolded α-Syn is degraded by neurons has been actively investigated. Investigations over the 45 last decades have elucidated that the ubiquitin-proteasome system (UPS) and the autophagy-46 lysosomal pathway (ALP) work in conjunction to degrade α-Syn 9, 10 . However, neither UPS n...

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Section: Methodsmentioning

confidence: 99%

Oligomeric α-synuclein-specific degradation by HtrA2/Omi to bestow a neuroprotective function

Chung

Mahm

Rahman

et al. 2018

Preprint

Self Cite

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“…After 2 weeks of acclimation, 80 mice were randomly divided into the following groups: Normal Formula Diet (Ctrl), NFD supplemented with resveratrol (RS), NFD in which the cornstarch and sucrose were replaced with Dongjin rice (DJ), NFD in which the cornstarch and sucrose were replaced with the resveratrol-enriched rice DJ526 (DJ526). The composition of the diets was described previously 18 . Resveratrol concentration in all the formulized diets was quantified by HPLC (ACQUITY UPLC, Waters, MA, USA) as described previously 16,17 .…”

Section: Methodsmentioning

confidence: 99%

“…We have previously published DJ526’s beneficial effects on aging, as well as the underlying mechanism of action 18 . DJ526 ameliorated age-related deterioration in mice, as demonstrated by inhibition of cutaneous tissue aging, and a boost in motor coordination and physical strength, whereas Dongjin rice or resveratrol on their own showed insignificant effects on age-related deterioration 18 .…”

Section: Background and Summarymentioning

confidence: 99%

“…Results of these data analyses were published in Scientific Reports , focusing on the interpretation of data, as well as elucidating the physiological impact of feeding mice with DJ526, Dongjin rice, and resveratrol 18 . In this manuscript, we describe the raw data from our microarray, blood serum and behavioral assays, which have each been deposited in public repositories.…”

Section: Background and Summarymentioning

confidence: 99%

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Gene expression profiles and physiological data from mice fed resveratrol-enriched rice DJ526

et al. 2016

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The molecular mechanism underlying lifespan extension by resveratrol remains widely discussed. To help study this mechanism, we previously created resveratrol-enriched rice, DJ526, by transferring the resveratrol biosynthesis gene into Dongjin rice. DJ526 accumulates 1.4–1.9 μg g−1 of resveratrol in its grain and can ameliorates age-related deterioration in mice, as compared to control animals, based on assessments of motor coordination, physical strength and cutaneous tissue aging. Here, we present raw data sets, deposited in public repositories, from microarray analysis and physiological data of mice fed with DJ526 and Dongjin rice and treated with resveratrol. We also provide a method to analyze blood serum at micron levels. These data sets may help other researchers find new clues regarding the etiology of the anti-aging process and signaling pathways induced by resveratrol, rice, or DJ526.

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“…The protocol was approved by the Ethics Committee of Chonbuk National University Laboratory Animal Center (Permit Number: CBU 2012-0040) in accordance with the 'Guide for the Care and Use of Laboratory Animals' , published by the National Research Council and endorsed by the ARRIVE Guidelines. The animal care protocol was followed as previously described [11][12][13][14].…”

Section: Animal Study Conditionmentioning

confidence: 99%

Iron-dextran as a thermosensitizer in radiofrequency hyperthermia for cancer treatment

2019

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Radiofrequency hyperthermia is a recently rediscovered oncotherapy rising in popularity. However, lack of a proper thermosensitizer limits current radiofrequency hyperthermia to be only slightly effective, mostly being used as a subsidiary to a standard oncotherapy. Here, we report that iron-dextran delivers iron ion to cancer cells for cancer-selective accumulation of the iron ion, which functions as a thermosensitizer for radiofrequency hyperthermia. Intravenous injection of iron-dextran to tumor-xenografted mice resulted in selective accumulation of iron ion in the targeted cancer cells. The accumulated iron ion in cancer cells dramatically reacted to radiofrequency wave to result in tumorselective dielectric temperature increment without harming the surrounding normal tissue. The oncotherapeutic effect of was evaluated using tumor-xenografted mice. The overall anticancer efficacy of radiofrequency hyperthermia after injection of iron-dextran as a thermosensitizer in breast cancer-bearing mice was much better than the efficacy of paclitaxel, a standard chemotherapy drug for cancer. Moreover, hyperthermia using iron-dextran as a thermosensitizer completely eradicated cancer in the tumor xenografted mice. This work suggests that iron-dextran is an ideal thermosensitizer for radiofrequency hyperthermia. We believe that the application of iron-dextran as a thermosensitizer would be a major progress in hyperthermia cancer treatments.

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The resveratrol-enriched rice DJ526 boosts motor coordination and physical strength

Cited by 21 publications

References 50 publications

Oligomeric α-synuclein-specific degradation by HtrA2/Omi to bestow a neuroprotective function

Oligomeric α-synuclein-specific degradation by HtrA2/Omi to bestow a neuroprotective function

Gene expression profiles and physiological data from mice fed resveratrol-enriched rice DJ526

Iron-dextran as a thermosensitizer in radiofrequency hyperthermia for cancer treatment

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