The response of skeletal muscle to alcohol abuse: Gender differences

Шенкман, Борис; Zinovyeva, O. E.; Belova, Svetlana P.; Самхаева, Н. Д.; Shcheglova, N. S.; Mirzoev, Timur M.; Vilchinskaya, Natalia A.; Altaeva, E. G.; Turtikova, O. V.; Nemirovskaya, T. L.

doi:10.1134/s0006350916050262

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…Interestingly, at 24 hours post alcohol, muscle fatigue was still enhanced in males, while females had completely recovered, indicating a sexually dimorphic response. This occurred despite reports of an increased propensity for females to develop chronic alcoholic injuries at lower cumulative doses of alcohol [ 10 , 21 , 22 ]. BAC’s were not different between male and female mice presently, indicating an equal dose of alcohol was administered to each sex.…”

Section: Discussionmentioning

confidence: 99%

Effects of alcohol on skeletal muscle contractile performance in male and female mice

et al. 2021

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Background Acute and chronic alcohol use can cause skeletal muscle myopathy in concert with impairments in skeletal muscle strength, function and fatigue resistance. However, the fundamental contractile deficits induced in the presence of alcohol versus those observed in the recovery period following the clearance of alcohol have not yet been characterized nor is it known whether sex influences these outcomes. Methods Male and female mice received an intraperitoneal injection of either saline (Control) or ethanol (EtOH; 5g/kg body weight). Muscle force, fatigue, fatigue recovery and twitch characteristics of the posterior crural muscle complex were measured in situ 1 hour and 24 hours post alcohol. Results In the presence of alcohol (1-hour post treatment) absolute and normalized force generated at 80–150 Hertz was decreased in male and female mice with concurrent reductions in the rate of force development and increases in ½ relaxation time. When expressed as a percentage of maximum force, both males and females also displayed an alcohol-induced leftward shift in the force frequency curve indicative of a type I contractile phenotype. Alcohol enhanced fatigue in both males and females but had no effect on force recovery. Following clearance of alcohol (24-hour post treatment), contractile function was completely restored in females while alcohol treated males experienced sustained reductions in absolute force and had enhanced fatigue compared with male controls. Conclusions In the presence of alcohol, both males and females exhibited significant declines in muscle force production and enhanced fatigue; however, following complete clearance of the alcohol, females recovered all functional parameters, while males did not.

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Section: Discussionmentioning

confidence: 99%

Effects of alcohol on skeletal muscle contractile performance in male and female mice

et al. 2021

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“…Mice were housed on a 12:12 light-dark cycle in a temperature-controlled (25°C) environment with ad libitum access to food and water. Female mice were chosen for experimentation as females have an increased propensity to develop chronic alcohol related injuries at lower cumulative doses of alcohol (Urbano-Marquez et al, 1995; Shenkman et al, 2016; DeGroat et al, 2018) and because the acute circadian studies had been performed in females allowing for comparisons to be made (Tice et al, 2021). This substrain of C57BL/6 mice was used to match our acute alcohol circadian experiments and we are not aware of any differences across substrain in skeletal muscle circadian response to alcohol to validate use of one substrain over another.…”

Section: Methodsmentioning

confidence: 99%

Chronic Alcohol Consumption Disrupts the Skeletal Muscle Circadian Clock in Female Mice

et al. 2022

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The intrinsic skeletal muscle core clock has emerged as a key feature of metabolic control and influences several aspects of muscle physiology. Acute alcohol intoxication disrupts the core molecular clock, but whether chronic consumption, like that leading to alcoholic myopathy, is also a zeitgeber for skeletal muscle remains unknown. The purpose of this work was to determine whether chronic alcohol consumption dysregulates the skeletal muscle core molecular clock and clock-controlled genes (CCGs). C57BL/6Hsd female mice (14 weeks old) were fed a control (CON) or alcohol (EtOH) containing liquid diet for 6 weeks. Gastrocnemius muscles and serum were collected from CON and EtOH mice every 4-h for 24-h. Chronic alcohol consumption disrupted genes of the core clock including suppressing the rhythmic peak of expression of Bmal1, Per1, Per2, and Cry2. Genes involved in the regulation of Bmal1 also exhibited lower rhythmic peaks including Reverb α and Myod1. The CCGs, Dbp, Lpl, Hk2, and Hadh were also suppressed by alcohol. The nuclear expression patterns of MYOD1, DBP, and REVERBα were shifted by alcohol, while no change in BMAL1 was detected. Overall, these data indicate that alcohol disrupted the skeletal muscle core clock but whether these changes in the core clock are causative or a consequence of alcoholic myopathy requires future mechanistic confirmation.

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