The response of human anulus fibrosus cells to cyclic tensile strain is frequency‐dependent and altered with disc degeneration

Gilbert, Hamish T. J.; Hoyland, Judith A.; Millward-Sadler, S J

doi:10.1002/art.27643

Cited by 62 publications

(110 citation statements)

References 40 publications

(60 reference statements)

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“…Similarly, some other studies have indicated that aggrecan is down-regulated in degenerated discs (Gruber et al, 2011;Park et al, 2011). In response to 1.0 Hz cyclic tensile stimulation (CTL), aggrecan gene expression did not change in degenerated IVDs after 1 or 3 h but demonstrated a sixfold decrease in gene expression after 24 h of loading (Gilbert et al, 2010). In a rabbit annular stab model of IVDD, degeneration associated with annular puncture resulted in a reduction in aggrecan mRNA to 35.5% at 3 weeks, which did not recover at subsequent time points (Sobajima et al, 2005).…”

Section: Aggrecanmentioning

confidence: 72%

“…In contrast, MMP9 gene expression increases in response to 0.33 Hz frequency. In contrast to anabolic genes, catabolic gene expression increases in response to loading at 0.33 Hz, which triggered the up-regulation of MMP13 in degenerated discs 3 h after loading with a return to normal expression levels after 24 h (Gilbert et al, 2010).…”

Section: Other Mmp Collaboration Findingsmentioning

confidence: 99%

“…In addition, there may be an up-regulation in ADAMTS28 and a down-regulation of ADAMTS17 in peripheral blood mononuclear cells (PBMCs) of degenerated discs (Zhang et al, 2010). Moreover, CTL causes a down-regulation in ADAMTS4 gene of nondegenerated AF cells (Gilbert et al, 2010).…”

Section: Aggrecanmentioning

confidence: 99%

“…A rabbit model demonstrated a decrease in collagen IIα mRNA 3 weeks after annular stab, which causes irreversible loss of NP cells (Sobajima et al, 2005). However, no significant differences have been shown between collagen II expression levels in normal and damaged human AF cells in response to cyclic tensile stain (Gilbert et al, 2010).…”

Section: Collagen IImentioning

confidence: 99%

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Genetic aspects of intervertebral disc degeneration

Hanaei

Abdollahzade

Khoshnevisan

et al. 2015

Reviews in the Neurosciences

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AbstractIntervertebral disc degeneration (IVDD) is one of the common causes of low back pain. Similar to many other multifactorial diseases, it is affected by environmental and genetic factors. Although not completely understood, genetic factors include a wide spectrum of variations, such as single nucleotide polymorphisms, which could play a significant role in the etiology of this disease. Besides, the interactions with environmental factors could make the role of genetic factors more complicated. Genetic variations in disc components could participate in developing degenerative disc disease through altering the normal homeostasis of discs. Gene polymorphisms in disc proteins (collagens I, II, III, IX, and XI), proteoglycans (aggrecan), cytokines (interleukins I, VI, and X), enzymes (matrix metalloproteinases II, III, and IX), and vitamin D receptor seem to play considerable roles in the pathology of this disease. There are also many other investigated genes that could somehow take part in the process. However, it seems that more studies are needed to clarify the exact role of genetics in IVDD.

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Section: Aggrecanmentioning

confidence: 72%

Section: Other Mmp Collaboration Findingsmentioning

confidence: 99%

Section: Aggrecanmentioning

confidence: 99%

Section: Collagen IImentioning

confidence: 99%

See 2 more Smart Citations

Genetic aspects of intervertebral disc degeneration

Hanaei

Abdollahzade

Khoshnevisan

et al. 2015

Reviews in the Neurosciences

View full text Add to dashboard Cite

show abstract

“…Thus, the loading regime in vitro was chosen to be within the physiological range. According to previous studies [Hamilton et al, 2004;Gilbert et al, 2010], we imposed 10% equibiaxial strain at 1.0 Hz for 12 h. Control PDLSCs were cultured without stretching.…”

Section: Application Of Mechanical Forcementioning

confidence: 99%

Mechanical Force-Induced Specific MicroRNA Expression in Human Periodontal Ligament Stem Cells

Wei

Wang

Ding

et al. 2014

Cells Tissues Organs

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It remains unclear how the expression of microRNAs (miRNAs) in human periodontal ligament stem cells (PDLSCs) might respond to mechanical stretch. To investigate specific miRNA expression in stretched PDLSCs, we used a Flexcell® FX-5000™ tension system to achieve external mechanical stimulation. Then, a custom-designed microarray assay was performed to investigate and describe the genome-wide differential expression of miRNAs in normal and stretched PDLSCs. Finally, we implemented integrative miRNA target prediction and network analysis approaches to construct an interaction network of the key miRNAs and their putative targets. We found that stretching induced morphological changes and increased alkaline phosphatase (ALP) activity, runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and bone sialoprotein (BSP) expression in PDLSCs. The microarray data showed that 53 miRNAs were differentially expressed with stretching. With an interaction network, we examined the connections between 10 selected key miRNAs and their putative target genes, which were related to mechanical force. The results from the interaction network provided a basis for postulating the functional roles of miRNAs in PDLSCs.

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Acute Vibration Induces Transient Expression of Anabolic Genes in the Murine Intervertebral Disc

McCann

Patel

Beaucage

et al. 2013

Arthritis & Rheumatism

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Objective. Low-amplitude whole-body vibration has been adopted for the treatment of back pain and spinal disorders. However, there is limited knowledge of the impact of vibration on the intervertebral disc (IVD). This study was undertaken to examine the effects of acute vibration on anabolic and catabolic pathways in the IVD and to characterize the dependence of these changes on time and frequency.Methods. Custom-designed platforms were developed to apply acute vibration to ex vivo and in vivo mouse models. Spinal segments (ex vivo) or mice (in vivo) were subjected to vibration (for 30 minutes at 15-90 Hz with peak acceleration at 0.3g), and IVDs were examined at specific time points after vibration. Gene expression was quantified using real-time polymerase chain reaction, and protein levels were examined by quantitative mass spectrometry and immunofluorescence.Results. In the ex vivo model, acute vibration at 15 Hz induced expression of anabolic genes (aggrecan, biglycan, decorin, type I collagen, and Sox9) and suppressed expression of Mmp13, with the most pronounced changes detected 6 hours following vibration. These beneficial effects were frequency dependent and were no longer evident between 45 and 90 Hz. In vivo, the effects on anabolic gene expression were even more robust and were accompanied by decreased expression of Adamts4, Adamts5, and Mmp3. Moreover, significant increases in the protein levels of aggrecan, biglycan, decorin, and type I collagen were detected in vivo.Conclusion. These findings demonstrate dramatic anabolic effects of acute vibration on IVD tissue, responses that are dependent on frequency. The similarity of the in vivo and ex vivo responses indicates that at least some effects of vibration are tissue autonomous.Back pain is the second most common chronic condition, with a reported lifetime incidence of 84% in industrialized countries (1). Degenerative disc disease is a major cause of back pain and results from changes in the composition of the extracellular matrix (ECM) within the intervertebral disc (IVD) (2). The IVD is maintained by specialized cell types that adjust their metabolism, and subsequently ECM composition, in response to external factors including nutritional, genetic, and biomechanical influences (3). The annulus fibrosus (AF) is composed of concentric lamellae formed by parallel bundles of type I collagen fibers, which provide tensile strength. In contrast, the central nucleus pulposus (NP) is composed of an irregular network of type II collagen (4), large aggregating proteoglycans (aggrecan), versican, and small proteoglycans

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The response of human anulus fibrosus cells to cyclic tensile strain is frequency‐dependent and altered with disc degeneration

Abstract: Conclusion. The response of human AF cells to CTS is frequency-dependent and is altered by degeneration.

Cited by 62 publications

References 40 publications

Genetic aspects of intervertebral disc degeneration

Genetic aspects of intervertebral disc degeneration

Mechanical Force-Induced Specific MicroRNA Expression in Human Periodontal Ligament Stem Cells

Acute Vibration Induces Transient Expression of Anabolic Genes in the Murine Intervertebral Disc

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