Mechanical Force-Induced Specific MicroRNA Expression in Human Periodontal Ligament Stem Cells

Wei, Fulan; Wang, J H; Ding, Guofu; Yang, Shuangyan; Li, Y; Hu, Yingyao; Wang, S L

doi:10.1159/000369613

Cited by 46 publications

(55 citation statements)

References 35 publications

(32 reference statements)

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“…Several miRNAs have been reported for their expression and roles in PDL and alveolar bones [115][116][117][118]. Under loading, several miRNAs in PDL and alveolar bone respond to the loading force and orientation of forces in different pattern of expression [119][120][121]. miRNA-21 has been shown to have critical roles in PDL, osteoblasts and osteoclasts [120,[122][123][124][125][126][127].…”

Section: Future Of Genetic Manipulation Of Tooth Movementmentioning

confidence: 99%

“…Under loading, several miRNAs in PDL and alveolar bone respond to the loading force and orientation of forces in different pattern of expression [119][120][121]. miRNA-21 has been shown to have critical roles in PDL, osteoblasts and osteoclasts [120,[122][123][124][125][126][127]. In addition, miRNA-21 deficient mouse demonstrated delayed tooth movement compared to the control mice via inhibition of osteoclastogenesis [127].…”

Section: Future Of Genetic Manipulation Of Tooth Movementmentioning

confidence: 99%

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Advances in Orthodontic Tooth Movement: Gene Therapy and Molecular Biology Aspect

Atsawasuwan¹,

Shirazi²

2019

Current Approaches in Orthodontics

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Accelerated orthodontic tooth movement has been recently the topic of interest for orthodontic practitioners. Increased numbers of both clinical and research articles associated with the accelerated orthodontic treatment have been published in peer-reviewed journals in the last couple of years. Biochemical approaches such as administration of drugs, vitamins, and proteins and/or physical approaches such as surgery, vibration, and photobiomodulation have been widely reported and demonstrated the predicted outcome; however, the results are controversial. Very few reports addressed on genetic background of patients or utilization of molecular biological approach on the accelerated orthodontic treatment. In this chapter, we will discuss about biology of tooth movement and how the advances in gene therapy and molecular biology technology would shape the future of orthodontic treatment.

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Section: Future Of Genetic Manipulation Of Tooth Movementmentioning

confidence: 99%

Section: Future Of Genetic Manipulation Of Tooth Movementmentioning

confidence: 99%

Advances in Orthodontic Tooth Movement: Gene Therapy and Molecular Biology Aspect

Atsawasuwan¹,

Shirazi²

2019

Current Approaches in Orthodontics

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show abstract

“…miRNAs bind to the specific mRNA recognition sequences that are located in the 3′ untranslated regions (3′ UTRs) of the target mRNA, which leads to mRNA degradation or protein translation interference [18]. Recent studies have demonstrated that miRNAs are differentially expressed between cyclically-stretched and non-stretched periodontal ligament stem cells (PDLSCs) [18,19,20], and the pattern is closely related to the mRNA expression of osteogenic markers, which indicates the important regulatory roles of miRNA in bone remodeling of orthodontic tooth movements. However, hitherto, the miRNA expression pattern of mature cementoblasts has rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

Screening the Expression Changes in MicroRNAs and Their Target Genes in Mature Cementoblasts Stimulated with Cyclic Tensile Stress

Wang

Cheng

et al. 2016

IJMS

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Cementum is a thin layer of cementoblast-produced mineralized tissue covering the root surfaces of teeth. Mechanical forces, which are produced during masticatory activity, play a paramount role in stimulating cementoblastogenesis, which thereby facilitates the maintenance, remodeling and integrity of cementum. However, hitherto, the extent to which a post-transcriptional modulation mechanism is involved in this process has rarely been reported. In this study, a mature murine cementoblast cell line OCCM-30 cells (immortalized osteocalcin positive cementoblasts) was cultured and subjected to cyclic tensile stress (0.5 Hz, 2000 µstrain). We showed that the cyclic tensile stress could not only rearrange the cell alignment, but also influence the proliferation in an S-shaped manner. Furthermore, cyclic tensile stress could significantly promote cementoblastogenesis-related genes, proteins and mineralized nodules. From the miRNA array analyses, we found that 60 and 103 miRNAs were significantly altered 6 and 18 h after the stimulation using cyclic tensile stress, respectively. Based on a literature review and bioinformatics analyses, we found that miR-146b-5p and its target gene Smad4 play an important role in this procedure. The upregulation of miR-146b-5p and downregulation of Smad4 induced by the tensile stress were further confirmed by qRT-PCR. The direct binding of miR-146b-5p to the three prime untranslated region (3′ UTR) of Smad4 was established using a dual-luciferase reporter assay. Taken together, these results suggest an important involvement of miR-146b-5p and its target gene Smad4 in the cementoblastogenesis of mature cementoblasts.

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“…Moreover, circRNAs have been proved to act as potent miRNAs sponges to regulate relevant genes (Hansen et al, 2013). Interestingly, previous studies revealed that 53 specific miRNAs were differentially expressed with MF, which implied that miRNAs may play a crucial part in the osteogenic differentiation of PDLSCs (F. L. Wei et al, 2014). They also found that the time-dependent increased miR-21 with MF could mediate the stretch-induced osteogenic differentiation of PDLSCs (F. Wei, et al, 2015).…”

mentioning

confidence: 99%

Identification and characterization of circular RNAs involved in mechanical force‐induced periodontal ligament stem cells

Wang

Cheng

Jin

et al. 2018

Journal Cellular Physiology

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Circular RNAs (circRNAs) play critical roles in signal transduction during cell proliferation, differentiation, and apoptosis in a posttranscriptional manner. Recently, circRNAs have been proved to be a large class of animal RNAs with regulatory potency. However, whether circRNAs can respond to mechanical force (MF) and impact on human periodontal ligament stem cells (PDLSCs) and the orthodontic tooth movement (OTM) process remain unknown. Here, we investigated the circRNAs expression patterns in PDLSCs induced by MF and found that circRNAs were responsive to the MF in PDLSCs. Through the valid reads’ distribution analysis, we found that the majority of reads in both the control PDLSCs and the MF‐induced PDLSCs were distributed in exons. Then we analyzed Gene Ontology terms of genes that overlap with or are neighbors of the stress‐responsive circRNAs and found unique enrichment patterns in biological processes, molecular function, and cellular component of PDLSCs. Next, we predicted the possible functions of circRNAs through circRNAs–miRNAs networks. We found that one circRNA may regulate one or several miRNA/miRNAs and one miRNA may interact with one or multiple circRNA/circRNAs. Importantly, a number of circRNAs were predicted to directly or indirectly regulate miRNAs‐mediated osteogenic differentiation in mesenchymal stem cells. For instance, circRNA3140 was highly and widely associated with microRNA‐21, which plays a critical role in MF‐induced osteogenic differentiation of PDLSCs. Taken together, these findings reveal a previously unrecognized mechanism that MF can induce the expression changes of circRNAs in PDLSCs, which may modulate the OTM process and the alveolar bone remodeling.

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Mechanical Force-Induced Specific MicroRNA Expression in Human Periodontal Ligament Stem Cells

Cited by 46 publications

References 35 publications

Advances in Orthodontic Tooth Movement: Gene Therapy and Molecular Biology Aspect

Advances in Orthodontic Tooth Movement: Gene Therapy and Molecular Biology Aspect

Screening the Expression Changes in MicroRNAs and Their Target Genes in Mature Cementoblasts Stimulated with Cyclic Tensile Stress

Identification and characterization of circular RNAs involved in mechanical force‐induced periodontal ligament stem cells

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