The response and resistance to drugs in ovarian cancer cell lines in 2D monolayers and 3D spheroids

Świerczewska, Monika; Sterzyńska, Karolina; Ruciński, Marcin; Andrzejewska, Małgorzata; Nowicki, Michał; Januchowski, Radosław

doi:10.1016/j.biopha.2023.115152

Cited by 13 publications

(7 citation statements)

References 111 publications

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“…This was also found in our study, as ATC spheroids generated from ATC01, 8505 C, and C643 cells were more resistant to dabrafenib and trametinib combination therapy than monolayer cultured cells. Drug resistance in tumor spheroids may better predict clinical response due to several reasons, including the spheroid structure (dense or loose), cell growth state (necrotic, quiescent, proliferating cells), or the diffusion of drugs into the dense cellular/extracellular matrix structure, and enrichment of cancer stem cell population [ 60 – 62 ]. On the other hand, the tumor spheroid models are enriched with cancer stem cells, this may also closely associate with treatment resistance [ 63 – 65 ].…”

Section: Discussionmentioning

confidence: 99%

Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics

Hu,

Liu,

Ghosh

et al. 2024

J Exp Clin Cancer Res

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Anaplastic thyroid cancer (ATC) is the most aggressive thyroid cancer. Despite advances in tissue culture techniques, a robust model for ATC spheroid culture is yet to be developed. In this study, we created an efficient and cost-effective 3D tumor spheroids culture system from human ATC cells and existing cell lines that better mimic patient tumors and that can enhance our understanding of in vivo treatment response. We found that patient-derived ATC cells and cell lines can readily form spheroids in culture with a unique morphology, size, and cytoskeletal organization. We observed both cohesive (dense and solid structures) and discohesive (irregularly shaped structures) spheroids within the same culture condition across different cell lines. BRAFWT ATC spheroids grew in a cohesive pattern, while BRAFV600E-mutant ATC spheroids had a discohesive organization. In the patient-derived BRAFV600E-mutant ATC spheroids, we observed both growth patterns, but mostly the discohesive type. Histologically, ATC spheroids had a similar morphology to the patient’s tumor through H&E staining and proliferation marker staining. Moreover, RNA sequencing analysis revealed that the gene expression profile of tumor cells derived from the spheroids closely matched parental patient tumor-derived cells in comparison to monolayer cultures. In addition, treatment response to combined BRAF and MEK inhibition in BRAFV600E-mutant ATC spheroids exhibited a similar sensitivity to the patient clinical response. Our study provides a robust and novel ex vivo spheroid model system that can be used in both established ATC cell lines and patient-derived tumor samples to better understand the biology of ATC and to test therapeutics.

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Section: Discussionmentioning

confidence: 99%

Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics

Hu,

Liu,

Ghosh

et al. 2024

J Exp Clin Cancer Res

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“…With advances in stem cell biology and organ regeneration, state-of-the-art 3D cell culture models can more accurately mimic the cellular microenvironment, cellular interactions, and physiological and biochemical reactions than single-layer 2D cell models [ 33 ]. Since the twenty-first century, this technology has gained popularity in many fields and it has been routinely used for the evaluation of cytotoxic T cell attacks, drug resistance, and tumor cell invasion, as well as revelation of their corresponding mechanisms [ [34] , [35] , [36] , [37] ].…”

Section: Models For Evaluation Of the Degree Of Tumor Penetrationmentioning

confidence: 99%

Enhancing drug penetration in solid tumors via nanomedicine: Evaluation models, strategies and perspectives

Shen,

Pan,

Gong

et al. 2024

Bioactive Materials

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“…One of the main advantages of using spheroids in cancer research is the ability to measure drug response. Multiple studies have been conducted involving spheroids derived from various types of cancer, including colorectal cancer [ 39 , 40 , 41 , 42 ], breast cancer [ 41 , 43 , 44 ], lung cancer [ 45 , 46 ], ovarian cancer [ 1 , 47 , 48 ] and even Ewing sarcoma cells [ 49 ]. It is important to note that 3D cultures, particularly spheroids, are a valuable and effective tool for studying cancer biology.…”

Section: 3d Cell Culturesmentioning

confidence: 99%

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Górnicki,

Lambrinow,

Golkar-Narenji

et al. 2024

Nanomaterials

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Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional cell cultivation methods. The most commonly used technique involves cultivating cells on a flat surface in a two-dimensional format due to its simplicity. A three-dimensional (3D) format can provide a microenvironment for surrounding cells. There are two main techniques for obtaining 3D structures based on the presence of scaffolding. Scaffold-free techniques consist of spheroid technologies. Meanwhile, scaffold techniques contain organoids and all constructs that use various types of scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels that are one of the most extensively studied forms of potential scaffolds for 3D culture up to 4D bioprinted biomaterials. 3D bioprinting is one of the most important techniques used to create biomimetic scaffolds. The versatility of this technique allows the use of many different types of inks, mainly hydrogels, as well as cells and inorganic substances. Increasing amounts of data provide evidence of vast potential of biomimetic scaffolds usage in tissue engineering and personalized medicine, with the main area of potential application being the regeneration of skin and musculoskeletal systems. Recent papers also indicate increasing amounts of in vivo tests of products based on biomimetic scaffolds, which further strengthen the importance of this branch of tissue engineering and emphasize the need for extensive research to provide safe for humansbiomimetic tissues and organs. In this review article, we provide a review of the recent advancements in the field of biomimetic scaffolds preceded by an overview of cell culture technologies that led to the development of biomimetic scaffold techniques as the most complex type of cell culture.

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The response and resistance to drugs in ovarian cancer cell lines in 2D monolayers and 3D spheroids

Cited by 13 publications

References 111 publications

Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics

Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics

Enhancing drug penetration in solid tumors via nanomedicine: Evaluation models, strategies and perspectives

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

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