The respiratory syncytial virus SH protein is incorporated into infectious virus particles that form on virus-infected cells

Huong, Tra Nguyen; Iyer, Laxmi; Lui, Jing; Wang, De Yun; Tan, Boon Huan; Sugrue, Richard J.

doi:10.1016/j.virol.2023.01.013

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…Indeed, the respiratory syncytial virus small hydrophobic (SH) protein has been shown to be modified with polylactosamines [26]. The function of this protein remains unclear, but it has been shown to be incorporated into viral particles [27]. Influenza B virus encodes a unique NB protein that has also been shown to harbour polylactosamine modifications and to be incorporated into the virion [28,29].…”

Section: Discussionmentioning

confidence: 99%

MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains

Juckel,

Desmarets,

Danneels

et al. 2023

Journal of General Virology

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Coronaviruses are positive-stranded RNA enveloped viruses. The helical nucleocapsid is surrounded by a lipid bilayer in which are anchored three viral proteins: the spike (S), membrane (M) and envelope (E) proteins. The M protein is the major component of the viral envelope and is believed to be its building block. The M protein of Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contains a short N-terminal domain with an N-glycosylation site. We investigated their N-glycosylation and show that polylactosamine chains are conjugated to SARS-CoV-2 and MERS-CoV M proteins in transfected and infected cells. Acidic residues present in the first transmembrane segments of the proteins are required for their glycosylation. No specific signal to specify polylactosamine conjugation could be identified and high mannose-conjugated protein was incorporated into virus-like particles.

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Section: Discussionmentioning

confidence: 99%

MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains

Juckel,

Desmarets,

Danneels

et al. 2023

Journal of General Virology

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“…Several viral pathogens, such as influenza virus, rhinovirus, metapneumovirus, coronavirus (including SARS-CoV-2), respiratory syncytial virus and some bacterial pathogens, primarily infect the host through the respiratory route. Respiratory pathogens cause ciliary impairments such as ciliated epithelial cell shedding, ciliary motility disruption, loss of cilia, other types of ciliary dysfunction and ciliogenesis suppression [12][13][14][15][16]. Viruses can enter host cells by recognizing various sialic acid receptors of airway epithelium cells [17,18].…”

Section: Introductionmentioning

confidence: 99%

Concurrent loss of ciliary genes WDR93 and CFAP46 in phylogenetically distant birds

Salve,

Kurian,

Vijay

2023

R. Soc. open sci.

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The respiratory system is the primary route of infection for many contagious pathogens. Mucociliary clearance of inhaled pathogens is an important innate defence mechanism sustained by the rhythmic movement of epithelial cilia. To counter host defences, viral pathogens target epithelial cells and cilia. For instance, the avian influenza virus that targets ciliated cells modulates the expression of WDR93 , a central ciliary apparatus C1d projection component. Lineage-specific prevalence of such host defence genes results in differential susceptibility. In this study, the comparative analysis of approximately 500 vertebrate genomes from seven taxonomic classes spanning 73 orders confirms the widespread conservation of WDR93 across these different vertebrate groups. However, we established loss of the WDR93 in landfowl, geese and other phylogenetically independent bird species due to gene-disrupting changes. The lack of WDR93 transcripts in species with gene loss in contrast to its expression in species with an intact gene confirms gene loss. Notably, species with WDR93 loss have concurrently lost another C1d component, CFAP46 , through large segmental deletions. Understanding the consequences of such gene loss may provide insight into their role in host–pathogen interactions and benefit global pathogen surveillance efforts by prioritizing species missing host defence genes and identifying putative zoonotic reservoirs.

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“…Several viral pathogens, such as influenza, rhinovirus, metapneumovirus, coronavirus (including SARS-CoV-2), respiratory syncytial virus (RSV), and some bacterial pathogens, primarily infect the host through the respiratory route. Respiratory pathogens cause ciliary impairments such as ciliated epithelial cell shedding, ciliary motility disruption, loss of cilia, other types of ciliary dysfunction, and ciliogenesis suppression [12][13][14][15][16]. Viruses can enter host cells through various sialic acid receptors of airway epithelium cells [17,18].…”

Section: Introductionmentioning

confidence: 99%

Concurrent loss of ciliary genesWDR93andCFAP46in phylogenetically distant birds

Girish,

Kurian,

Vijay

2023

Preprint

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The respiratory system is the primary route of infection for many contagious pathogens. Mucociliary clearance of inhaled pathogens is an important innate defense mechanism sustained by the rhythmic movement of epithelial cilia. To counter host defenses, viral pathogens target epithelial cells and cilia. For instance, the influenza virus modulates the expression of WDR93, a central ciliary apparatus C1d projection component. Lineage-specific prevalence of such host defense genes results in differential susceptibility. Our comparative analysis of ~500 vertebrate genomes supports the widespread conservation of WDR93. However, we identified loss of the WDR93 in landfowl, geese, and other phylogenetically-independent bird species. Notably, species with WDR93 loss have concurrently lost another C1d component, CFAP46, through large segmental deletions. Understanding the consequences of such gene loss may provide insight into their role in host-pathogen interactions and benefit global pathogen surveillance efforts by prioritizing species missing host defense genes and identifying putative zoonotic reservoirs.

show abstract

The respiratory syncytial virus SH protein is incorporated into infectious virus particles that form on virus-infected cells

Cited by 4 publications

References 38 publications

MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains

MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains

Concurrent loss of ciliary genes WDR93 and CFAP46 in phylogenetically distant birds

Concurrent loss of ciliary genesWDR93andCFAP46in phylogenetically distant birds

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