The respiration of yeast at different concentrations of glucose

Aldous, J. G.; Fisher, Kenneth C.; Stern, Joseph R.

doi:10.1002/jcp.1030350302

Cited by 10 publications

(2 citation statements)

References 8 publications

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“…Aerobic fermentation was negligible below 0.1 per cent and above this concentration became quite significant, although for 1.0 per cent glucose the carbon dioxide produced by fermentation was only about 20 per cent of the oxygen consumed by respiration. The results of Aldous et al (17) and of Stickland (10) differ from those of the present report in which the rate of aerobic fermentation is seen to be five times that of respiration and independent of the concentration of glucose. In neither of these earlier papers are there presented time-course curves of aerobic fermentation and the duration of this process for any concentration of glucose is not given.…”

Section: Discussioncontrasting

confidence: 94%

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Aerobic Fermentation and the Depletion of the Amino Acid Pool in Yeast Cells

Swenson

Betts

1963

The Journal of General Physiology

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The amino acid pool of yeast ceils, Saccharomyces cerevisiae, incubated with galactose remains at a constant level for 100 minutes. This is 30 minutes beyond the time at which the oxidative phase of the induced-enzyme formation begins. Washed yeast cells, the pools of which have been depleted 60 per cent by incubation with glucose, do not replenish their pools as do washed cells incubated without a substrate. These facts indicate that the induced enzymes are formed at least partially from pool-replenishing amino acids. The time of onset of pool depletion is the time at which the aerobic fermentation phase of induced-enzyme formation begins for cells incubated with galactose. With 0.1 per cent galactose the respiratory phase begins at 100 minutes but no aerobic fermentation nor pool depletion occurs. The rates of respiration and aerobic fermentation are constant for four glucose concentrations from 0.1 to 1.0 per cent. The amount of aerobic fermentation is proportional to the initial concentration of glucose. Amino acid pool depletion occurs for all concentrations but depletion ceases and is followed by pool replenishment after aerobic fermentation is complete. Ultraviolet radiations, which delay the appearance of the respiratory phase of induced-enzyme formation, completely eliminate both the appearance of aerobic fermentation and pool depletion. The results indicate an intimate association between aerobic fermentation and amino acid pool depletion.Previous work by Swenson and Dott (1) has shown that in the absence of an exogenous energy source, amino acids leak slowly from yeast cells and that accompanying this leakage there is a slow increase in the free amino acid pool level of the cells. The addition of glucose greatly increases the metabolic rate, resulting in the leakage of fewer amino acids and in the depletion of the amino acid pool by about 50 per cent after 2 hours. Pool depletion during glucose metabolism was interpreted to be the result of protein synthesis. If glucose is removed from the suspending medium after the pool level has been

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Section: Discussioncontrasting

confidence: 94%

“…Aldous et al (17) were the first to make concurrent measurements in yeast cells of oxygen consumption and carbon dioxide production using different concentrations of glucose. They found that with concentrations of 0.1 per cent glucose and below, no aerobic fermentation occurred.…”

Section: Discussionmentioning

confidence: 99%

Aerobic Fermentation and the Depletion of the Amino Acid Pool in Yeast Cells

Swenson

Betts

1963

The Journal of General Physiology

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Photodynamic effects on metabolism and reproduction in yeast

Freeman

Giese

1952

J. Cell. Comp. Physiol.

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Effects of anesthetics on oxygen consumption and on synaptic transmission in sympathetic ganglia

Larrabee

Ramos

Bülbring

1952

J. Cell. Comp. Physiol.

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EDITH BULBRINGPre-eminent among contemporary hypotheses concerning the mode of action of anesthetics on nerve cells is one which supposes that the depressant effects of these substances are due to an interference with cellular metabolism. This interference is assumed to result from inactivation of certain enzymes, which are as yet only partly identified. It is appropriate that this hypothesis receive extensive consideration and test a t a time when considerable effort is being devoted to elucidation of the role of enzymes in controlling the chemical reactions and functional properties of living cells.Experimental evidence that anesthetics interfere with metabolism has been obtained mostly from measurements of oxygen consumption. The observations of Quastel and his colleagues, and subsequent confirmations and extensions by other laboratories, have abundantly demonstrated that a wide

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The respiration of yeast at different concentrations of glucose

Cited by 10 publications

References 8 publications

Aerobic Fermentation and the Depletion of the Amino Acid Pool in Yeast Cells

Aerobic Fermentation and the Depletion of the Amino Acid Pool in Yeast Cells

Photodynamic effects on metabolism and reproduction in yeast

Effects of anesthetics on oxygen consumption and on synaptic transmission in sympathetic ganglia

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