The resorption of nanocrystalline calcium phosphates by osteoclast-like cells

Detsch, Rainer; Hagmeyer, Daniel; Neumann, Markus; Schaefer, S.; Vortkamp, Andrea; Wuelling, Manuela; Ziegler, G.; Epple, Matthias

doi:10.1016/j.actbio.2010.03.003

Cited by 88 publications

(75 citation statements)

References 54 publications

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“…Interestingly, but in spite on the fact that the biological apatite of bones contains the substantial amount of carbonates, among investigated samples of nanocrystalline apatites, osteoclastic differentiation was found to be constrained on carbonate-rich samples, leading to smaller numbers of osteoclast-like cells and fewer resorption pits. Furthermore, the highest resorption rate was found for nanodimensional HA with a low carbonate content, which strongly stimulated the differentiation of osteoclast-like cells on its surface [674].…”

Section: Nanodimensional and Nanocrystalline Calcium Orthophosphates mentioning

confidence: 98%

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

AJBE

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Nano-sized particles and crystals play an important role in the formation of calcified tissues of various animals. For example, nano-sized and nanocrystalline calcium orthophosphates in the form of apatites of biological origin represent the basic inorganic building blocks of bones and teeth of mammals. Namely, according the recent developments in biomineralization, tens to hundreds nanodimensional crystals of a biological apatite are self-assembled into these complex structures. This process occurs under a strict control by bioorganic matrixes. Furthermore, both a greater viability and a better proliferation of various types of cells have been detected on smaller crystals of calcium orthophosphates. Thus, the nano-sized and nanocrystalline forms of calcium orthophosphates have a great potential to revolutionize the hard tissue-engineering field, starting from bone repair and augmentation to controlled drug delivery systems. This review reports on current state of the art and recent developments on the subject, starting from synthesis and characterization to biomedical and clinical applications. Furthermore, the review also discusses possible directions for future research and development.

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Section: Nanodimensional and Nanocrystalline Calcium Orthophosphates mentioning

confidence: 98%

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

AJBE

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“…Resorption implies an initial tight attachment of osteoclasts to the disk surface to create the "sealing zone", rich in F-actin, which isolates the resorptive space from the surrounding material. 11,12 The ruffled border is formed by fusion of intracellular acidic vesicles which contain enzymes (such as cathepsin K), Cl − and H + ions which are released into the resorption lacunae for the acidification to a pH of around 4.5. [13][14][15] This process produces the dissolution of the material, which is then endocytosed from the resorption cavity and transported to the secretory domain for releasing into the extracellular environment.…”

Section: Intracellular Ros and Camentioning

confidence: 99%

“…The osteoclast thus isolates the resorptive space from the surrounding bone. 11,12 The ruffled border is formed by fusion of intracellular acidic vesicles which form finger-like projections inside the sealing zone. The vesicles contain a cocktail of matrix-degrading enzymes (such as cathepsin K), hydrogen ions (H + ) and chloride ions (Cl − ) which are released into the resorption lacunae and are responsible for acidification to a pH of around 4.5.…”

Section: Introductionmentioning

confidence: 99%

Nanocrystalline silicon substituted hydroxyapatite effects on osteoclast differentiation and resorptive activity

Matesanz¹,

Linares

Lilue³

et al. 2014

J. Mater. Chem. B

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In the present study, the effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline Si-substituted hydroxyapatite (nano-SiHA) on osteoclast differentiation and resorptive activity have been evaluated in vitro using osteoclast-like cells. The action of these materials on proinflammatory and reparative macrophage populations was also studied. NanoSiHA disks delayed the osteoclast differentiation and decreased the resorptive activity of these cells on their surface, as compared to nano-HA samples, without affecting cell viability. Powdered nano-SiHA also induced an increase of the reparative macrophage population. These results along with the beneficial effects on osteoblasts previously observed with powdered nano-SiHA suggest the potential of this biomaterial for modulating the fundamental processes of bone formation and turnover, preventing bone resorption and enhancing bone formation at implantation sites in treatment of osteoporotic bone and in bone repair and regeneration.

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“…U-937 and RAW 264.7 monocytic-macrophage leukemia cells, fibroblasts, endothelial cells and primary osteoclast progenitors have been carried out with several of the above bone substitute materials to not only assess osteoclast differentiation and osteoclast activity but also secretion of cellular factors [2]. Specifically, the RAW 264.7 murine leukemia cell line has been used as an important in vitro cell culture model for studying activation and differentiation into osteoclastlike cells [9,10]. For example, when RAW 264.7 cells were grown for 14 days on HAp and stimulated with RANKL, differentiation via cell fusion occurred into osteoclast-like multinucleated cells [9].…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, the RAW 264.7 murine leukemia cell line has been used as an important in vitro cell culture model for studying activation and differentiation into osteoclastlike cells [9,10]. For example, when RAW 264.7 cells were grown for 14 days on HAp and stimulated with RANKL, differentiation via cell fusion occurred into osteoclast-like multinucleated cells [9]. Additionally, 3D cell culture scaffolds composed of biphasic calcium phosphate (BCP) biomaterial, human plasma clots, and RAW 264.7 cells stimulated for 2 days in the presence of a transfected overexpression vector containing a glutathione-S transferase (GST)-RANKL fusion protein demonstrated significantly increased expression of Vegfa and Vegfc compared to untreated controls [11].…”

Section: Introductionmentioning

confidence: 99%

Nanoscale Bioactive Glass Activates Osteoclastic Differentiation of RAW 264.7 Cells

Detsch

Rübner

Strissel

et al. 2016

Nanomedicine (Lond.)

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(215 words)Key words: osteoclasts; biomaterials; 45S5 nanoscaled (n) Bioactive glass (BG) 2 AbstractPresently, there is limited knowledge regarding the differentiation of osteoclasts in the presence of nanoscale bioactive glasses (nBG). This investigation examined increasing concentrations of 45S5 nBG and its influence on osteoclast differentiation. After 14 days of culturing cells with 500 µg/ml nBG viability of ~100% was detected, however DNA synthesis was reduced supporting the differentiation into osteoclast-like cells. RNA activation of nine genes occurred in a nBG concentration dependent manner. Low concentrations of nBG increased expression of genes involved in commitment to cell fusion. High concentration of nBG increased gene expression supporting osteoclast-like differentiation. We conclude that nBG enhances RAW 264.7 osteoclast differentiation especially controlling gene expression in a concentration dependent manner, which could reflect normal regulation during bone growth.Background: There is limited knowledge regarding differentiation of osteoclasts in the presence of nanoscale bioactive glasses (nBG). This investigation examined increasing concentrations of 45S5 nBG and its influence on osteoclast differentiation. Materials and Methods:Different concentrations of 45S5 nBG were cultured up to 14 days with the murine RAW264.7 cell line and human primary monocytes and M-CSF and RANKL.

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The resorption of nanocrystalline calcium phosphates by osteoclast-like cells

Cited by 88 publications

References 54 publications

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Nanocrystalline silicon substituted hydroxyapatite effects on osteoclast differentiation and resorptive activity

Nanoscale Bioactive Glass Activates Osteoclastic Differentiation of RAW 264.7 Cells

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