Intracellular
reactive oxygen species and reactive sulfur play
a vital role in regulating redox homeostasis and maintaining cell
functions. Sulfur dioxide (SO2) has emerged as an important
gas signal molecule recently, which is not only a potential reducing
agent but also a potential inductor of oxidative stress in organisms.
Due to high reactivity, peroxynitrite (ONOO–) could
act on many biomolecules, such as proteins, lipids, and nucleic acids,
and cause irreversible damage, eventually leading to cell apoptosis
or necrosis. In order to further illuminate the dichotomous role of
SO2 under oxidative stress induced by ONOO–, we designed the first dual-site fluorescent sensor (NIR-GYf) for separate or continuous detection of SO2 and ONOO–. NIR-GYf was successfully used for cell
imaging of endogenous SO2 and ONOO–.
In addition, western blotting analysis was used to verify the oxidation
and antioxidation of SO2 and its dichotomous biological
influence. Finally, NIR-GYf was integrated with multiple
Boolean logic operations to construct an advanced analysis device,
thereby realizing the direct analysis of SO2 and ONOO– levels.