The requirement of chrysobactin dependent iron transport for virulence incited by Erwinia chrysanthemi on Saintpaulia ionantha

Enard, Corine; Franza, Thierry; Neema, Claire; Gill, Paul R.; Persmark, Magnus; Neilands, J. B.; Expert, Dominique

doi:10.1007/bf00011882

Cited by 17 publications

(3 citation statements)

References 37 publications

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“…While siderophore-mediated iron-uptake is involved in phytopathogenesis of Erwinia chrysanthemi (Enard et al, 1988(Enard et al, , 1991Masclaux and Expert, 1995;Neema et al, 1993) and apparently in E. rhapontici and E. persicinus (Feistner et al, 1996) it has not been implicated in the phytopathogeneses of Agrobacterium tumefaciens (Leong and Neilands, 1981), and E. carotovora subsp. carotovora, (Bull and Loper, 1996) or U. maydis (Leong et al, 1988;Mei et al, 1993;Mei and Leong, 1994).…”

Section: Introductionmentioning

confidence: 98%

Siderophore accumulation and phytopathogenicity in Microbotryum violaceum

Birch

Ruddat

2005

Fungal Genetics and Biology

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Section: Introductionmentioning

confidence: 98%

Siderophore accumulation and phytopathogenicity in Microbotryum violaceum

Birch

Ruddat

2005

Fungal Genetics and Biology

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“…Ferric chrysobactin is transported back into the bacterial cell via its specific TonB-dependent outer membrane receptor Fct and a cytoplasmic membrane permease. − Moreover, ferric enterobactin is used as an exogenous iron carrier by E. chrysanthemi cells, and its transport occurs via another TonB-dependent receptor immunologically related to the Escherichia coli ferric Enterobactin receptor FepA . On the other hand, the same permease mediates the transport of the ferric complexes of these two siderophores, indicating a low specificity for the passage of these chelates through the cytoplasmic membrane.…”

Section: Introductionmentioning

confidence: 99%

Iron(III) Uptake and Release by Chrysobactin, a Siderophore of the Phytophatogenic Bacterium Erwinia chrysanthemi

et al. 2008

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The plant pathogenic enterobacterium Erwinia chrysanthemi causes important soft-rot disease on a wide range of plants including vegetables and ornamentals of economic importance. It produces a major mono(catecholate) siderophore, chrysobactin (alpha-N-(2,3-dihydroxybenzoyl)-D-lysyl-L-serine). To unravel the role of chrysobactin in the virulence of E. chrysanthemi, its iron(III) coordination properties were thus investigated in aqueous solutions using electrospray ionization mass spectrometric, potentiometric, and spectrophotometric methods. Moreover, kinetic experiments allowed us to determine the uptake and release mechanisms. The formation mechanism of the 1:1 complex reveals a key role of the terminal carboxylic group of chrysobactin in the binding of either FeOH(2+) or Fe2(OH)2(4+). The proton-driven dissociation of the ferric tris-, bis-, and mono(chrysobactin) complexes was also studied. For these three ferric complexes, a single protonation triggers the release of the bound chrysobactin molecule. Interestingly, the dissociation of the last ligand proceeded via the formation of an intermediate for which a salicylate-type mode of bonding was proposed.

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“…Enterobactin is not synthesized by E. chrysanthemi cells, but promotes growth of a chrysobactindeficient mutant if supplied exogenously. An iron-regulated outer membrane protein with an apparent molecular mass of 88,000 Da and immunologically related to the E. coli ferric enterobactin receptor FepA is thought to play a similar function in E. chrysanthemi (21). In addition, E. chrysanthemi strain 3937 produces another high-affinity iron uptake system mediated by a citrate siderophore called achromobactin (22).…”

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Chrysobactin-dependent Iron Acquisition inErwinia chrysanthemi

Rauscher

Expert

Matzanke

et al. 2002

Journal of Biological Chemistry

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Under iron limitation, the plant pathogen Erwinia chrysanthemi produces the catechol-type siderophore chrysobactin, which acts as a virulence factor. It can also use enterobactin as a xenosiderophore. We began this work by sequencing the 5-upstream region of the fct-cbsCEBA operon, which encodes the ferric chrysobactin receptor and proteins involved in synthesis of the catechol moiety. We identified a new iron-regulated gene (cbsH) transcribed divergently relative to the fct gene, the translated sequence of which is 45.6% identical to that of Escherichia coli ferric enterobactin esterase. Insertions within this gene interrupt the chrysobactin biosynthetic pathway by exerting a polar effect on a downstream gene with some sequence identity to the E. coli enterobactin synthase gene. These mutations had no effect on the ability of the bacterium to obtain iron from enterobactin, showing that a functional cbsH gene is not required for iron removal from ferric enterobactin in E. chrysanthemi. The cbsH-negative mutants were less able to utilize ferric chrysobactin, and this effect was not caused by a defect in transport per se. In a nonpolar cbsH-negative mutant, chrysobactin accumulated intracellularly. These defects were rescued by the cbsH gene supplied on a plasmid. The amino acid sequence of the CbsH protein revealed characteristics of the S9 prolyl oligopeptidase family. Ferric chrysobactin hydrolysis was detected in cell extracts from a cbsHpositive strain that was inhibited by diisopropyl fluorophosphate. These data are consistent with the fact that chrysobactin is a D-lysyl-L-serine derivative. Mö ssbauer spectroscopy of whole cells at various states of 57 Felabeled chrysobactin uptake showed that this enzyme is not required for iron removal from chrysobactin in vivo. The CbsH protein may therefore be regarded as a peptidase that prevents the bacterial cells from being intracellularly iron-depleted by chrysobactin.

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The requirement of chrysobactin dependent iron transport for virulence incited by Erwinia chrysanthemi on Saintpaulia ionantha

Cited by 17 publications

References 37 publications

Siderophore accumulation and phytopathogenicity in Microbotryum violaceum

Siderophore accumulation and phytopathogenicity in Microbotryum violaceum

Iron(III) Uptake and Release by Chrysobactin, a Siderophore of the Phytophatogenic Bacterium Erwinia chrysanthemi

Chrysobactin-dependent Iron Acquisition inErwinia chrysanthemi

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