The Renal Proximal Tubule

Silverman, Melvin; Turner, R. Jay

doi:10.1007/978-1-4615-6564-2_1

Cited by 10 publications

(6 citation statements)

References 114 publications

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“…In cultured rat astrocytes, the efflux of the MeHg-glutathione complex was found to be mediated by a probenecidsensitive organic acid transport system (Fujiyama et al, 1994). Since this would be an active transport system (Silverman and Turner, 1979;Steinberg et al, 1987), DNP should be able to block the MeHg efflux, thereby resulting in an increased MeHg accumulation. In contrast to this, Kerper et al (1996) found that the export of MeHg-glutathione complexes in brain capillary endothelial cells did not require ATP.…”

Section: Discussionmentioning

confidence: 99%

Uptake of HgCl2and MeHgCl in an Insect Cell Line (Aedes albopictusC6/36)

Braeckman

Cornelis

Rzeznik

et al. 1998

Environmental Research

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Section: Discussionmentioning

confidence: 99%

Uptake of HgCl2and MeHgCl in an Insect Cell Line (Aedes albopictusC6/36)

Braeckman

Cornelis

Rzeznik

et al. 1998

Environmental Research

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“…A glycoprotein interacting with the cytoskeleton of microvilli has been isolated from the intestinal brush border (12), and a polar localization of glycoproteins has been observed in various organs (13)(14)(15)(16)(17). Especially relevant to this point ai studies on the budding of enveloped viruses from various surfaces of polarized epithelial cells in culture.…”

Section: Resultsmentioning

confidence: 99%

Lumen formation and redistribution of inframembranous proteins during differentiation of ducts in the rat mammary gland.

Dulbecco¹,

Allen²,

Bowman³

1984

Proc. Natl. Acad. Sci. U.S.A.

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During the development of the rat mammary gland, ducts are formed from end-buds, which contain the stem cells. In this process a lumen is formed in the semisolid mass of the end-bud, and the cells acquire polarity. We have studied this process by following the localization of three inframembranous proteins present in the cells'of both end-buds and ducts; microvillin, the microvillar protein p80, and the desmosomal plaque protein p205. We find that the development of ducts is accompanied by a redistribution of these proteins, which in immature parts of the end-buds are found together in the cell. Microvillin and p80 go together to the apical pole of the cells, in contact with the lumen, whereas p205 goes to the basal surface, in contact with cells of the myoepithelial lineage. The acquisition of polarity occurs at the same time as a lumen begins to form by local'gaps between clls. It seems likely that the redistribution of the inframembraneous proteins is the consequence of the localization of surface glycoproteins that affect in opposite ways the adhesion between the cells. characterized. In these cells microvillin is an inframembranous protein present, in association with other proteins, in microvilli. We show here that the distribution of microvillin in cells of end-buds and ducts of the rat mammary gland undergoes changes correlated with the formation of the lumen. The changes concern not only the cell types in which the protein is expressed but also the part of the cell in which it is localized. 13y using the monoclonal antibody it is possiThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. 5763 ble to follow the redistribution of components of the outer layer of the cytoplasm that accompanies the development of cell polarity and lumen formation.In this study we have also used antibodies to two other proteins with inframembranous localization: a protein of the brush border, which is also present in the microvilli of mammary cells (p80) (4), and a component of demosomal plaques (p205) (5). We show that they also change localization within cells as they differentiate. The pattern of redistribution of p80 is exactly the same as that of microvillin, whereas that of p205 differs, taking a complementary path.MATERIALS AND METHODS Animals. Sprague-Dawley rats, either 3 or 7 weeks old, were used. Cryostat sections were cut at 5 ,tm thickness from whole fourth and fifth glands frozen at -30'C.Reagents. Rabbit antisera to the microvillar 80-kDa protein was generously provided by A. Bretscher (4), to the demosomal plaque 205-kDa protein by D. R. Garrod (6), and to collagen IV by L. A. Liotta. Monoclonal antibody 9B16-12 specific for microvillin has been described (3). Immunofluorescence. Immunofluorescence was carried out by the sandwich technique as described (1). RESULTSIn normal mammary glands of 3-or 7-week-old rats microvillin is restricted to the epi...

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“…The human kidney has around 1.3 x 10 6 functioning units [nephrons]. Each day 160-170L of plasma ultrafiltrate passes across the glomerulus into the kidney at a rate of approximately 1.5 nL /sec per nephron [14]. The ratio of filtered phosphate to plasma phosphate concentration is between 0.89-0.96 [1].…”

Section: Introductionmentioning

confidence: 99%

“…If it is, do PKA and Epacoperate an activation/inhibitory partnership to regulate PTH activity?(12) The dysfunctional protein activity which is being highlighted in GWAS studies of calcium stone formers. (13) Whether MEPE has a physiological role in the kidneys (14). The on-going research into the molecular mechanisms of renal phosphate absorption is revealing an amazingly intricate system which has evolved over millions of years.…”

mentioning

confidence: 99%

The Intricacies of Renal Phosphate Reabsorption—An Overview

Walker

2024

Preprint

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To maintain an optimal body content of phosphorus throughout postnatal life, variable phosphate absorption from food must be finely matched with urinary excretion. This amazing feat is accomplished through synchronised phosphate transport by myriads of ciliated cells lining the renal proximal tubules. These respond in real time to changes in the phosphate and composition of the renal filtrate, and to hormonal instructions. How they do this has stimulated decades of research. New analytical techniques, coupled with incredible advances in computer technology, have opened new avenues for investigation at a sub-cellular level. There has been a surge of research into different aspects of the process. These have verified long held beliefs and are also dramatically extending our vision of the intense, integrated, intracellular activity which mediates phosphate absorption. Already, some have indicated new approaches for pharmacological intervention to regulate phosphate in common conditions, including chronic renal failure and osteoporosis, as well as rare inherited biochemical disorders. It is a rapidly evolving field. The aim here is to provide an overview of our current knowledge, to show where it is leading, and where there are uncertainties. Hopefully this will raise questions and stimulate new ideas for further research.

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The Renal Proximal Tubule

Cited by 10 publications

References 114 publications

Uptake of HgCl2and MeHgCl in an Insect Cell Line (Aedes albopictusC6/36)

Uptake of HgCl2and MeHgCl in an Insect Cell Line (Aedes albopictusC6/36)

Lumen formation and redistribution of inframembranous proteins during differentiation of ducts in the rat mammary gland.

The Intricacies of Renal Phosphate Reabsorption—An Overview

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