The Remineralization of EDTA–Treated Human Dentine

Kawasaki, Koji; Ruben, J.; Stokroos, I.; Takagi, Okiuji; Arends, J

doi:10.1159/000016529

Cited by 69 publications

(62 citation statements)

References 15 publications

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“…There was a concern that no direct comparison was performed between 30-s immersion and 24-h immersion in the Control group. It should be noted that the immersion of EDTA-demineralized dentin in artificial saliva for up to several days or weeks did not induce remineralization [31][32][33] . On the other hand, there is a study revealed that demineralized dentin immersed in artificial saliva for up to 21 days showed constant (slight) activity of endogenous collagenases, comparing to 1-day immersion 34) .…”

Section: Discussionmentioning

confidence: 95%

Effects of zinc fluoride on inhibiting dentin demineralization and collagen degradation <i>in vitro</i>: A comparison of various topical fluoride agents

et al. 2016

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Root caries is developed because of demineralization followed by enzymatic collagen degradation. This in vitro study aimed to examine the inhibitory efficacy of ZnF2 on dentin demineralization and collagen degradation. Bovine dentin specimens were treated either with ZnF2 or HCl-acidified ZnF2 (ZnF2/HCl) and then demineralized. Anti-demineralization efficacy was assessed by TMR as mineral loss (ΔZ). The efficacy was compared with silver diammine fluoride (SDF), KF, and acidulated phosphate fluoride (APF). For evaluating anti-collagen degradation, EDTA-demineralized dentin specimens were treated by one of four fluoride agents [SDF, APF, ZnF2/HCl, NaF] followed by collagenase challenge. The eroded depth of collagen layer in the lesion was assessed using optical microscope. ΔZ of SDF, KF, ZnF2/HCl, and APF were significantly lower compared with ZnF2 and Control (no treatment). Regarding anti-collagen degradation, SDF and ZnF2/HCl demonstrated a significant difference in the eroded depth compared with Control. Although SDF possessed higher efficacy, ZnF2/HCl might be beneficial as a staining-free agent.

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Section: Discussionmentioning

confidence: 95%

Effects of zinc fluoride on inhibiting dentin demineralization and collagen degradation <i>in vitro</i>: A comparison of various topical fluoride agents

et al. 2016

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“…The prolonged application of EDTA resulted in a similar decrease of the CS, confirming previous data on the ability of EDTA to remove dentin noncollagenous proteins. 36 It has been demonstrated previously that because of its high affinity with water, CS morphologic status depends on the tissue hydration level. 16 Thus we can hypothesize that phosphoric acid, which draws water from the tissue because of its osmolarity, 18 greatly affects the three-dimensional status of CS.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical analysis of dentin: A double‐labeling technique

Breschi

Gobbi

Lopes

et al. 2003

J Biomedical Materials Res

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Immunocytochemical analysis is a fundamental and selective technique for identifying different molecular components of human dental structure. The hypothesis tested here is that the application of different etching solutions on dentin does not hinder collagen fibrils and proteoglycans from maintaining their immunochemical antigenicity. Human dentin disks were treated with 0.5M of EDTA, citric acid, maleic acid, or phosphoric acid (for 15 or 30 s). A double-immunolabeling technique was performed to identify, simultaneously, collagen fibrils and chondroitin sulfate. The use of different acids resulted in different degrees of labeling. Maleic and citric acids revealed a diffuse and intense labeling for both collagen fibrils and proteoglycans. The use of phosphoric acid on dentin showed a massive coagulation of the proteoglycans (15 s) or very low labeling (30 s). These data clarify that the use of acids on dentin components is able to modify their antigenicity. Moreover, the double-labeling immunocytochemical technique allows understanding of the spatial relationships between the collagen fibrils and proteoglycans of the dentin matrix.

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“…Dentin remineralization by STMP associated with a calcium solution by the formation of calcium phosphate crystals was previously reported [Zhang et al, 2012] and is also supported by our results. The combination of STMP with calcium hydroxide (Ca(OH) 2 ) might also favor dentin remineralization by the formation of a calcium phosphate precursor that promotes conditions for mineral crystal growth required for remineralization [Kawasaki et al, 1999;Featherstone, 2000]. The phosphorylation of proteins present on the surface of an organic template by STMP could trap calcium ions by electrostatic force resulting in prenucleation clusters [Wang et al, 2001;George and Veis, 2008].…”

Section: Discussionmentioning

confidence: 99%

Sodium Trimetaphosphate as a Novel Strategy for Matrix Metalloproteinase Inhibition and Dentin Remineralization

Gonçalves

Scaffa²,

Giacomini³

et al. 2018

Caries Res

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The effect of sodium trimetaphosphate (STMP) as an antiproteolytic and remineralizing agent on demineralized dentin was evaluated in vitro. The inhibitory potential of STMP at 0.5, 1.5, 3.5, and 5% against recombinant matrix metalloproteinases (MMPs) MMPs-2 and -9 was assessed by zymography. To investigate its remineralization potential, 40 bovine root specimens were obtained and subjected to a demineralization protocol to produce caries-like dentin lesions. After that, dentin surfaces were divided into 3 areas: (1) mineralized (no treatment); (2) demineralized; and (3) demineralized/treated with STMP and submitted to a pH-cycling associated or not with STMP (1.5, 3.5, or 5% STMP, 10 min of treatment). After that, superficial hardness (SH) and cross-sectional hardness (CSH) were determined. Polarized light microscopy (PLM) was used to qualitatively evaluate mineralization within the caries-like lesions. The zymographic analysis showed that STMP solution is a potent inhibitor of the gelatinolytic activity of MMPs-2 and -9 depending on the dose, since the lowest concentration (0.5%) partially inhibited the enzyme activity, while the higher concentrations completely inhibited enzyme activity. Regarding remineralization effect, only 1.5% STMP solution enhanced both the SH and CSH. PLM showed that the area treated with 1.5% STMP presented similar birefringence as mineralized sound dentin. In conclusion, 1.5% STMP solution is effective as an antiproteolytic agent against MMPs and promotes dentin remineralization.

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The Remineralization of EDTA–Treated Human Dentine

Cited by 69 publications

References 15 publications

Effects of zinc fluoride on inhibiting dentin demineralization and collagen degradation <i>in vitro</i>: A comparison of various topical fluoride agents

Effects of zinc fluoride on inhibiting dentin demineralization and collagen degradation <i>in vitro</i>: A comparison of various topical fluoride agents

Immunocytochemical analysis of dentin: A double‐labeling technique

Sodium Trimetaphosphate as a Novel Strategy for Matrix Metalloproteinase Inhibition and Dentin Remineralization

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