The Relevance of Angiogenesis in Benign and Malignant Epithelial Tumors of the Ovary: A Quantitative Histologic Study

Brustmann, Hermann; Riss, P.; Naudé, S. M.

doi:10.1006/gyno.1997.4815

Cited by 45 publications

(25 citation statements)

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“…AQP1 was not expressed in the tumor cell cytoplasm. These findings are consistent with previous reports and support the theory that AQP1 has a role in transvascular water flow and fluid transport of tumor cells in EOC patients (22,29). Our findings also suggest that malignant ovarian tumors have increased vascular permeability, like other tumor types (30).…”

Section: Discussionsupporting

confidence: 93%

“…AQP1 was localized in the microvessel epithelium in these cases. Additionally, the expression of AQP1 was demonstrated on the membranes of interstitial cells of ovarian cancer tissue, and rarely on tumor cell membranes (22,29). In our study, strong AQP1 protein expression was found in all primary serous EOC microvessels and small vessels.…”

Section: Discussionsupporting

confidence: 45%

“…In another study, increased expression of AQP1 was demonstrated in all stages of colon cancer (28). Previous studies have demonstrated that AQP1 protein is strongly expressed in small vessels, but not in the cytoplasm, in nearly all EOC types (22,29). AQP1 was localized in the microvessel epithelium in these cases.…”

Section: Discussionmentioning

confidence: 67%

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Does Aquaporin-1 expression have clinical significance in serous epithelial ovarian cancer?

Takal¹,

Baykal²,

Başer³

et al. 2013

J Turkish German Gynecol Assoc

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Objective: To assess the relationship between Aquaporin-1 (AQP1) expression and clinicopathological variables in serous epithelial ovarian cancer (EOC). Material and Methods:Serous EOC cases treated in our institution between January 2007 and December 2009 were included in the study. A semi-quantitative immunohistochemical method was used to determine AQP1 expression levels, intratumoral microvessel density (IMD) and AQP1/IMD ratios. The relationship between these parameters and clinicopathological variables were assessed. P values less than 0.05 was considered statistically significant. Results:A total of 55 cases of serous EOC were included in the study.AQP1 was strongly expressed in the membranes of microvessels and small vessels within all tumor tissues. In a few cases, AQP1 expression was also observed in the membrane of interstitial cells and in individual tumor cells. A positive correlation was detected between preoperative CA125 levels and the expression of AQP1 (R: 0.277, p<0.05). AQP1 expression was similar between FIGO stage I-II and FIGO stage III-IV cases (p > 0.05). A significant relationship did not exist between AQP1 expression and FIGO stage, lymph node metastasis or ascites volume (p>0.05). Conclusion:In this study, AQP1 expression did not have a significant association with important clinicopathological variables in serous EOC. Future studies are needed to determine AQP1 expression in other histological types of EOC. (J Turkish-German Gynecol Assoc 2013; 14: 130-5) Key words: Aquaporin-1, intratumoral microvessel density, serous epithelial ovarian cancer Received: 15 April, 2013 Accepted: 16 May, 2013 Amaç: Seröz epitelyal over kanserlerinde (EOK) Aquaporin-1 (AQP1) ekspresyonunun klinik ve patolojik değişkenlerle ilişkisini araştırmaktır. Gereç ve Original Investigation 130 IntroductionEpithelial ovarian cancer (EOC) is the most lethal gynecologic cancer, with a five-year overall survival of approximately 31 to 53%, considering all stages (1-3). Generally accepted clinicopathological variables that may have an impact on survival are tumor histology, disease stage, patient age, performance status of the patient, presence of ascites, residual tumor burden after surgery and preoperative CA-125 level (4-16).Aquaporins are a group of homologous water channel proteins, which are expressed in various tissues (17-19). Aquaporin-1 (AQP1) has important functions in various organs such as the kidney, central and peripheral nervous system, eye, lacrimal glands, salivary glands, lungs, pleura, gastrointestinal system, female and male reproductive system, inner ear and skin. Considering its function in angiogenesis, the possible role of AQP1 in carcinogenesis, tumor progression and metastasis ability has been an area of interest (20,21 A previous study has demonstrated that there is a strong positive correlation between AQP1 expression and intratumoral microvessel density (IMD) in EOC (22). In the present study, we evaluated AQP1 expression in serous EOC, in order to determine its association with...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 45%

See 1 more Smart Citation

Does Aquaporin-1 expression have clinical significance in serous epithelial ovarian cancer?

Takal¹,

Baykal²,

Başer³

et al. 2013

J Turkish German Gynecol Assoc

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“…They lack smooth muscle in their walls, demonstrate an irregular course and arteriovenous shunt formation. [13][14][15][16] In addition, malignant tumor vessels generally have low impedance, which causes high diastolic flow and low systolic-diastolic variation. Some differentiation between benign and malignant masses is achieved by quantifying these differences.…”

Section: Introductionmentioning

confidence: 99%

Adnexal Masses in Perimenopausal Women: How Effective is Color Flow Mapping and Pulse Doppler Waveform Studies in detecting Malignancy Preoperatively?

Gupta¹

2013

Journal of SAFOMS

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Objective: This prospective study was undertaken to assess the sensitivity and specificity of color flow mapping and pulse Doppler waveform in identifying malignancy preoperatively in adnexal masses in perimenopausal women. Materials and methods:A total of 100 cases with adnexal masses in perimenopausal women were scanned, underwent color flow mapping and pulsed Doppler waveform studies. These cysts were divided into three groups according to their ultrasound appearance -cystic, mixed cystic and solid. All the adnexal mases were removed surgically and sent for histopathological study. Results:The sensitivity and specificity of color flow mapping in identifying malignant ovarian tumors was 63.6 and 87.0% respectively. In the case of solid cysts, color flow mapping identified all malignant tumors. But, color flow mapping missed malignancies in cystic and mixed cystic masses. To improve detection rates, we used Doppler waveform studies and used resistance index less than 0.4 as the indicator of malignancy and found overall sensitivity and specificity of 90.9 and 92.3%, which was higher than that of color flow mapping. Conclusion:To conclude ultrasonic color flow mapping and pulse Doppler waveform studies used preoperatively on adnexal masses in perimenopausal women are widely available, performed rapidly and relatively easily, cost-effective and a reliable noninvasive method to predict malignancy preoperatively.

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“…This process, called angiogenesis, is critical to tumorigenicity and metastasis (10). Similarly to carcinogenesis, angiogenesis is a multistep process, regulated by a balance between stimulatory and inhibitory factors released by the tumor and its microenvironment (7,(11)(12)(13)(14)(15)(16)(17).Angiogenesis facilitates tumor growth through a series of steps including dissociation of endothelial cells (EC) from adjacent perycites, remodelling of extracellular matrix, proliferation and migration of EC and capillary differentiation.Nitric oxide (NO) is a signalling molecule produced by three isoforms of nitric oxide synthases (neuronal NOS, endothelial NOS and inducible NOS); it mediates a variety of actions such as vasodilatation, neurotransmission, host defence against bacteria and tumor cells (18,19).Strong evidence suggests that NO is a regulator of angiogenesis (20,21), which enhances vascular permeability, induces extracellular matrix degradation, endothelial cell proliferation and migration (22)(23)(24) and stimulates the expression of vascular growth factor (VEGF) (25,26). Increased iNOS expression and NO production in BPH and in high grade PC occur when compared to normal tissue (27).…”

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confidence: 99%

The DDAH/NOS pathway in human prostatic cancer cell lines: Antiangiogenic effect of L-NAME

Sorrenti¹

2011

Int J Oncol

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Abstract. Benign prostate hypertrophy (BPH) and prostate cancer (PC) are prostate chronic diseases that require a long period for development from a small lesion to clinical manifestation. PC is the most common cancer in men in Europe and the Americas. Tumor growth and metastasis depend upon the development of neovasculature around the tumor. This process, called angiogenesis, may be regulated by NO, and thus modulation of NO production could play an important role in tumor progression. Recent studies report the involvement of DDAH, an enzyme which metabolizes the endogenous NOS inhibitor ADMA, in the development of tumor vasculature. The aim of the present study was to verify the involvement of the DDAH/ NOS pathway in the progression of prostate cancer. The effect of the NOS inhibitor L-NAME was evaluated in the human prostate cancer cell line LnCap and in BPH-1 cells which represent benign prostatic hypertrophy. Higher DDAH-2, eNOS, iNOS and VEGF expression was found in LnCap cells compared to BPH-1 cells. L-NAME treatment of LnCap cells resulted in a reduction in VEGF, iNOS and eNOS expression. VEGF, iNOS and eNOS inhibition is a promising approach for targeting tumor vasculature and certain NOS inhibitors could potentially serve as experimental agents for treatment of certain chemoresistant tumors, including prostate tumors. Moreover, since in our experimental conditions L-NAME was unable to reduce DDAH activity and expression, it is plausible to hypothesize the development of a targeted polypharmacological approach by developing dual and specific inhibitors of DDAH and NOS to better control NO biosynthesis. IntroductionBenign prostate hypertrophy (BPH) and prostate cancer (PC) are prostate chronic diseases that require a long period for development from a small lesion to clinical manifestation. PC is the second leading cause of death in men of Western world (1) Despite significant improvements in local and systemic therapies, most deaths from prostate cancer are due to metastasis which resist conventional therapies (2-4). Therefore, novel therapeutic strategies targeting specific molecular markers are being pursued to allow early detection and cure.Tumor growth and metastasis depend upon the development of a neovasculature around the tumor (5-9). This process, called angiogenesis, is critical to tumorigenicity and metastasis (10). Similarly to carcinogenesis, angiogenesis is a multistep process, regulated by a balance between stimulatory and inhibitory factors released by the tumor and its microenvironment (7,(11)(12)(13)(14)(15)(16)(17).Angiogenesis facilitates tumor growth through a series of steps including dissociation of endothelial cells (EC) from adjacent perycites, remodelling of extracellular matrix, proliferation and migration of EC and capillary differentiation.Nitric oxide (NO) is a signalling molecule produced by three isoforms of nitric oxide synthases (neuronal NOS, endothelial NOS and inducible NOS); it mediates a variety of actions such as vasodilatation, neurotransmission, host defence a...

show abstract

The Relevance of Angiogenesis in Benign and Malignant Epithelial Tumors of the Ovary: A Quantitative Histologic Study

Cited by 45 publications

References 0 publications

Does Aquaporin-1 expression have clinical significance in serous epithelial ovarian cancer?

Does Aquaporin-1 expression have clinical significance in serous epithelial ovarian cancer?

Adnexal Masses in Perimenopausal Women: How Effective is Color Flow Mapping and Pulse Doppler Waveform Studies in detecting Malignancy Preoperatively?

The DDAH/NOS pathway in human prostatic cancer cell lines: Antiangiogenic effect of L-NAME

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